Construction of recombinant Escherichia coli for enhanced bioconversion of colchicine into 3-demethylated colchicine at 70l bioreactor level

Process Biochemistry

Volumn 45, Issue 7, 2010, Pages 1036-1042

  Dubey, Kashyap Kumar ^a   Haque, Shafiul ^b   Jawed, Arshad ^c   Singh, Bhanu P ^a   Behera, B K ^a  

^a MAHARSHI DAYANAND UNIVERSITY   (India)

^b JAMIA MILLIA ISLAMIA CENTRAL UNIVERSITY   (India)

^c INSTITUTE OF MICROBIAL TECHNOLOGY   (India)

Author keywords

Biocatalyst; Bioreactor; Colchicine derivatives; CYP3A4 enzyme; Dissolved oxygen (DO); Scale up

Indexed keywords

BACILLUS MEGATERIUM; COLCHICINE DERIVATIVES; CYP3A4 ENZYME; DEMETHYLATION; E. COLI; EXPRESSION VECTORS; IMPELLER TIP VELOCITY; OPTIMIZED CONDITIONS; PRODUCT TOXICITY; PROTEIN EXPRESSIONS; RECOMBINANT E. COLI; RECOMBINANT ESCHERICHIA COLI; RECOMBINANT STRAINS; SCALE-UP; SCALE-UP FACTOR; SHAKE FLASKS; SUBSTRATE TOXICITY; VOLUMETRIC OXYGEN TRANSFER COEFFICIENT; VOLUMETRIC PRODUCTIVITY; WORKING VOLUME;

BACTERIOLOGY; BIOCONVERSION; BIOREACTORS; BOTTLES; CELL CULTURE; DISSOLUTION; ENZYME ACTIVITY; ENZYMES; ESCHERICHIA COLI; GENE EXPRESSION; OPTIMIZATION; SUBSTRATES; TOXICITY;

DISSOLVED OXYGEN;

EID: 77954815822     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2010.03.014     Document Type: Article

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