Scale-up from shake flasks to fermenters in batch and continuous mode with Corynebacterium glutamicum on lactic acid based on oxygen transfer and pH

Biotechnology and Bioengineering

Volumn 98, Issue 4, 2007, Pages 800-811

  Seletzky, Juri M ^a   Noak, Ute ^a   Fricke, Fens ^a   Welk, Eike ^a   Eberhard, Werner ^a   Knocke, Christof ^a   Büchs, Jochen ^a  

^a RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

Corynebacterium glutamicum; Gas liquid mass transfer; pH modeling; Scale up; Shake flask; Volumetric mass transfer coefficient

Indexed keywords

BIOMASS; LACTIC ACID; MASS TRANSFER; MATHEMATICAL MODELS; OXYGEN; PH EFFECTS;

CORYNEBACTERIUM GLUTAMICUM; GAS LIQUID MASS TRANSFER; PH MODELING; SHAKE FLASK; VOLUMETRIC MASS TRANSFER COEFFICIENT;

FERMENTERS;

LACTIC ACID;

AEROBIC CELL CULTURE; ARTICLE; BIOMASS; CONTROLLED STUDY; CORYNEBACTERIUM GLUTAMICUM; FERMENTATION TECHNIQUE; NONHUMAN; OXYGEN CONSUMPTION; OXYGEN TRANSPORT; PH MEASUREMENT; TITRIMETRY; VOLUMETRY;

BIOREACTORS; CELL CULTURE TECHNIQUES; COMPUTER SIMULATION; CORYNEBACTERIUM GLUTAMICUM; FERMENTATION; HYDROGEN-ION CONCENTRATION; INDUSTRIAL MICROBIOLOGY; LACTIC ACID; MODELS, BIOLOGICAL; OXYGEN; PILOT PROJECTS;

CORYNEBACTERIUM GLUTAMICUM;

EID: 35648998052     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21359     Document Type: Article

References (49)

1. Akgün A, Maier B, Preis D, Roth B, Klingelhöfer R, Büchs J. 2004. A novel parallel shaken bioreactor system for continuous operation. Biotechnol Prog 20:1718-1724.
2. Akita K. 1981. Diffusivities of gases in aqueous solutions. Ind Eng Chem Fundam 20:89-94.
3. Alves SS, Maia CI, Vasconcelos JMT. 2004: Gas-liquid mass transfer coefficient in stirred tanks interpreted through contamination kinetics. Chem Eng Process 43:823-830.
4. Alves SS, Orvalho SP, Vasconcelos JMT. 2005. Effect of bubble contamination on rise velocity and mass transfer. Chem Eng Sci 60:1-9.
5. Amanullah A, Blair R, Nienow AW, Thomas CR. 1999. Effects of agitation intensity on mycelial morphology and protein production in chemostat cultures of recombinant Aspergillus oryzae. Biotechnol Bioeng 62:434-446.
6. Anderlei T, Büchs J. 2001. Device for sterile online measurement of the oxygen transfer rate in shaking flasks. Biochem Eng J 7:157-162.
7. Anderlei T, Zang W, Papaspyrou M, Büchs J. 2004. Online respiration activity measurement (OTR, CTR, RQ) in shake flasks. Biochem Eng J 17:187-194.
8. Bröer S, Krämer R. 1991. Lysine excretion by Corynebacterium glutamicum. 1. Identification of a specific secretion carrier system. Eur J Biochem 202:131-135.
9. Büchs J. 2001. Introduction to advantages and problems of shaken cultures. Biochem Eng J 7:91-98.
10. Büchs J, Maier U, Milbradt C, Zoels B. 2000a. Power consumption in shaking flasks on rotary shaking machines. Part I. Power consumption measurement in unbaffled flasks at low liquid viscosity. Biotechnol Bioeng 68:589-593.
11. Büchs J, Maier U, Milbradt C, Zoels B. 2000b. Power consumption in shaking flasks on rotary shaking machines. Part II. Nondimensional description of specific power consumption and flow regimes in unbaffled flasks at elevated liquid viscosity. Biotechnol Bioeng 68:594-601.
12. Chamsartra S, Hewitt CJ, Nienow AW. 2005. The impact of fluid mechanical stress on Corynebacterium glutamicum during continuous cultivation in an agitated bioreactor. Biotechnol Lett 27:693-700.
13. Clark GJ, Langley D, Bushell ME. 1995. Oxygen limitation can induce microbial secondary metabolite formation: Investigations with miniature electrodes in shaker and bioreactor culture. Microbiology 141:663-669.
14. Cocaign M, Monnet C, Lindley ND. 1993. Batch kinetics of Corynebacterium glutamicum during growth on various carbon substrates: Use of mixtures to localize metabolic bottlenecks. Appl Microbiol Biotechnol 40:526-530.
15. Cocaign-Bousquet M, Lindley ND. 1995. Pyruvate overflow and carbon flux within the central metabolic pathways of Corynebacterium glutamicum during growth on lactate. Enzyme Microb Technol 17:260-267.
16. Cui YQ, van der Lans RGJM, Luyben KChAM. 1998. Effects of dissolved oxygen tension and mechanical forces on fungal morphology in submerged fermentation. Biotechnol Bioeng 57:409-419.
17. Dawson RMC. 1986. Data for Biochemical Research. Oxford: Clarendon Press.
18. Gupta A, Rao G. 2003. A study of oxygen transfer in shake flasks using a non-invasive oxygen sensor. Biotechnol Bioeng 84:351-358.
19. Gustafsson TK, Waller KV. 1992. Nonlinear and adaptive control of pH. Ind Eng Chem Res 31:2681-2698.
20. Gustafsson TK, Skrifvars BO, Sandström KV, Waller KV. 1995. Modelling pH for control. Ind Eng Chem Res 34:820-827.
21. Henzler HJ, Schedel M. 1991. Suitability of shaking flask for oxygen supply to microbiological cultures. Bioprocess Eng 7:123-131.
22. Katzer W, Blackburn M, Charman K, Martin S, Penn J, Wrigley S. 2001. Scale-up of filamentous organisms from tubes and shake-flasks into stirred vessels. Biochem Eng J 7:127-134.
23. Kawase Y, Harlard B, Moo-Young M. 1992. Liquid-phase mass transfer coefficients in bioreactors. Biotechnol Bioeng 39:1133-1140.
24. Kennedy MJ, Reader SL, Davies J, Rhoades DA, Silby HW. 1994. The scale up of myceleal shake flask fermentations: A case study of gamma linolenic acid production by Mucor hiemalis IRL 51. J Indust Microbiol 13:212-216.
25. Kim CH, Rao KJ, Youn DJ, Rhee SK. 2003. Scale-up of recombinant hirudin production from Saccharomyces cerevisiae. Biotechnol Bioprocess Eng 8:303-305.
26. Kinoshita S, Udaka S, Shimono M. 1957. Amino acid fermentation. I. Production of L-glutamic acid by various microorganisms. J Gen Appl Microbiol 3:193-205.
27. Lide. 1999. Handbook of Chemistry and Physics. Boca Raton: CRC Press.
28. Maier U. 2002. Gas-Flüssigkeits-Stofftransfer im Schüttelkolben. PhD thesis RWTH Aachen University. Aachen: Shaker Verlag, . p.
29. Maier U, Büchs J. 2001. Characterization of the gas-liquid mass transfer in shaking bioreactors. Biochem Eng J 7:99-106.
30. Maier U, Losen M, Büchs J. 2004. Advances in understanding and modeling the gas-liquid mass transfer in shake flasks. Biochem Eng J 17:155-167.
31. Mrotzek C, Anderlei T, Henzler HJ, Büchs J. 2001. Mass transfer resistance of sterile plugs in shaking bioreactors. Biochem Eng J 7:107-112.
32. Nicolas-Simonnot MO, Sardin M, Grévillot G. 1995. Consequences of ion-exchange and sorption effects on pH waves propagating in a strong-base anion-exchange column. J Liquid Chromatogr 18:463-487.
33. Owens JD, Legan JD. 1987. Determination of the Monod substrate saturation constant for microbial growth. FEMS Microbiol Rev 46:419-432.
34. Peter CP, Lotter S, Maier U, Büchs J. 2004. Impact of out-of-phase conditions on screening results in shaking flasks experiments. Biochem Eng J 17:205-215.
35. Peter CP, Suzuki Y, Büchs J. 2006. Hydromechanical stress in shake flasks: Correlation for the maximum local energy dissipation rate. Biotechnol Bioeng 93:1164-1176.
36. Rischbieter E, Schumpe A. 1996. Gas solubilities in aqueous solutions of organic substances. J Chem Eng Data 41:809-812.
37. Sahoo S, Verma RK, Suresh AK, Rao KK, Bellare J, Suraishkumar GK. 2003. Macro-level and genetic-level responses of Bacillus subtilis to shear stress. Biotechnol Prog 19:1689-1696.
38. 2 solubilities in fermentation media. Biotechnol Bioeng 21:1075-1078.
39. Schumpe A, Quicker G, Deckwer WD. 1982. Gas solubilities in microbial culture media. Adv Biochem Eng 48:1-38.
40. Schwarz A, Mösche M, Wittenberg A, Jördening H-J, Buchholz K, Reuss M. 1997. Mathematical modelling and simulation of an industrial scale fluidized bed reactor for anaerobic wastewater treatment scale-up effect on pH-gradients. Wat Sci Tech 36:219-227.
41. Seletzky JM, Noack U, Fricke J, Hahn S, Büchs J. 2006. Metabolic activity of Corynebacterium glutamicum grown on L-lactic acid under stress. Appl Biotechnol Bioeng 72:1297-1307.
42. Shah AH, Hameed A, Ahmad S, Khan GM. 2002. Optimization of culture conditions for L-lysine fermentation by Corynebacterium glutamicum. OnLine J Biol Sci 2:151-156.
43. Sipkema EM, De Koning W, Ganzeveld KJ, Janssen DB, Beenackers A. 1998. Experimental pulse technique for microbial kinetics in continuous culture. J Biotechnol 64:159-176.
44. Stöckmann C, Maier U, Anderlei T, Knocke C, Gellissen G, Büchs J. 2003. The oxygen transfer rate as key parameter for the characterization of Hansenula polymorpha screening cultures. J Ind Microbiol Biotechnol 30:613-622.
45. Støle-Hansen K, Foss BA. 1997. Controlling pH in a precipitation process. Model Identification Control 18:261-272.
46. Sumino Y, Sonoi K, Doi M. 1993. Scale-up of purine nucleoside fermentation from shaking flask to a stirred-tank fermentor. Appl Microbiol Biotechnol 38:581-585.
47. Van't Riet K. 1979. Review of Measuring methods and results in nonviscous gas-liquid mass transfer in stirred vessels. Ind Eng Chem Process Des Dev 18:357-364.
48. Vasconcelos JM, Orvalho SP, Alves SS. 2002. Gas-liquid mass transfer to single bubbles: Effect of surface contamination. AIChE J 48:1145-1154.
49. Westerlund T, Skrifavars B, Karilla S. 1985. On the uniqueness in pH calculations. Chem Eng Sci 40:973-976.