Knockdown of the Dickkopf 3 gene induces apoptosis in a lung adenocarcinoma

International Journal of Molecular Medicine

Volumn 26, Issue 1, 2010, Pages 33-38

  Il, Lae Jung ^a,b   Hyo, Jin Kang ^a   Kug, Chan Kim ^a   In, Gyu Kim ^a  

^a Korea Atomic Energy Research Institute   (South Korea)

^b Chonbuk National University   (South Korea)

Author keywords

Apoptosis; Dickkopf 3 gene; Lung adenocarcinoma; Methylation; p53; Reactive oxygen species

Indexed keywords

BISULFITE; CYCLIN D1; CYCLIN E; DICKKOPF 1 PROTEIN; DICKKOPF 2 PROTEIN; DICKKOPF 3 PROTEIN; DICKKOPF 4 PROTEIN; GLYCOPROTEIN; PROTEIN BAX; PROTEIN P21; PROTEIN P53; REACTIVE OXYGEN METABOLITE; SMALL INTERFERING RNA; UNCLASSIFIED DRUG; WNT PROTEIN;

APOPTOSIS; ARTICLE; CELL GROWTH; CELL LEVEL; CELL PROLIFERATION; CELL SURVIVAL; CLONOGENIC ASSAY; CONTROLLED STUDY; DNA MODIFICATION; DNA SYNTHESIS; FLOW CYTOMETRY; GENE EXPRESSION; GENE FUNCTION; GENE SILENCING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HUMAN TISSUE; LUNG ADENOCARCINOMA; LUNG NON SMALL CELL CANCER; LUNG SMALL CELL CANCER; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; RECEPTOR DOWN REGULATION; TUMOR CELL LINE; WESTERN BLOTTING;

APOPTOSIS; BCL-2-ASSOCIATED X PROTEIN; BLOTTING, WESTERN; CELL LINE, TUMOR; CELL PROLIFERATION; CELL SURVIVAL; CYCLIN-DEPENDENT KINASE INHIBITOR P21; DNA METHYLATION; FLOW CYTOMETRY; HUMANS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; LUNG NEOPLASMS; REACTIVE OXYGEN SPECIES; RNA INTERFERENCE; TIME FACTORS; TUMOR SUPPRESSOR PROTEIN P53;

EID: 77954715979     PISSN: 11073756     EISSN: 1791244X     Source Type: Journal    
DOI: 10.3892/ijmm-00000431     Document Type: Article

References (39)

1. Glinka A, Wu W, Delius H, Monaghan AP, Blumenstock C and Niehrs C: Dickkopf-1 is a member of a new family of secreted proteins and functions in head induction. Nature 391: 357-362, 1998.
2. Krupnik VE, Sharp JD, Jiang C, Robison K, Chickering TW, Amaravadi L, Brown DE, Guyot D, Mays G, Leiby K, Chang B, Duong T, Goodearl AD, Gearing DP, Sokol SY and McCarthy SA: Functional and structural diversity of the human Dickkopf gene family. Gene 238: 301-313, 1999.
3. He X, Semenov M, Tamai K and Zeng X: LDL receptor-related proteins 5 and 6 in Wnt/beta-catenin signaling: arrows point the way. Development 131: 1663-1677, 2004.
4. Mao B, Wu W, Li Y, Hoppe D, Stannek P, Glinka A and Niehrs C: LDL-receptor-related protein 6 is a receptor for Dickkopf proteins. Nature 411: 321-325, 2001.
5. Mao B and Niehrs C: Kremen2 modulates Dickkopf2 activity during Wnt/LRP6 signaling. Gene 302: 179-183, 2003.
6. Lee EJ, Jo M, Rho SB, Park K, Yoo YN, Park J, Chae M, Zhang W and Lee JH: DKK3, down-regulated in cervical cancer, functions as a negative regulator of beta-catenin. Int J Cancer 124: 287-297, 2009.
7. Nozaki I, Tsuji T, Iijima O, Ohmura Y, Andou A, Miyazaki M, Shimizu N and Namba M: Reduced expression of REIC/Dkk-3 gene in non-small cell lung cancer. Int J Oncol 19: 117-121, 2001.
8. Kobayashi K, Ouchida M, Tsuji T, Hanafusa H, Miyazaki M, Namba M, Shimizu N and Shimizu K: Reduced expression of the REIC/Dkk-3 gene by promoter-hypermethylation in human tumor cells. Gene 282: 151-158, 2002.
9. Tsuji T, Nozaki I, Miyazaki M, Sakaguchi M, Pu H, Hamazaki Y, Iijima O and Namba M: Antiproliferative activity of REIC/Dkk-3 and its significant down-regulation in non-small-cell lung carcinomas. Biochem Biophys Res Commun 289: 257-263, 2001.
10. Kurose K, Sakaguchi M, Nasu Y, Ebara S, Kaku H, Kariyama R, Arao Y, Miyazaki M, Tsushima T, Namba M, Kumon H and Huh NH: Decreased expression of REIC/Dkk-3 in human renal clear cell carcinoma. J Urol 171: 1314-1318, 2004.
11. Hsieh SY, Hsieh PS, Chiu CT and Chen WY: Dickkopf-3/REIC functions as a suppressor gene of tumor growth. Oncogene 23: 9183-9189, 2004. (Pubitemid 40069688)
12. Roman-Gomez J, Jimenez-Velasco A, Agirre X, Castillejo JA, Navarro G, Barrios M, Andreu EJ, Prosper F, Heiniger A and Torres A: Transcriptional silencing of the Dickkopfs-3 (Dkk-3) gene by CpG hypermethylation in acute lymphoblastic leukaemia. Br J Cancer 91: 707-713, 2004.
13. Kawano Y, Kitaoka M, Hamada Y, Walker MM, Waxman J and Kypta RM: Regulation of prostate cell growth and morphogenesis by Dickkopf-3. Oncogene 25: 6528-6537, 2006.
14. Lodygin D, Epanchintsev A, Menssen A, Diebold J and Hermeking H: Functional epigenomics identifies genes frequently silenced in prostate cancer. Cancer Res 65: 4218-4227, 2005.
15. Urakami S, Shiina H, Enokida H, Kawakami T, Kawamoto K, Hirata H, Tanaka Y, Kikuno N, Nakagawa M, Igawa M and Dahiya R: Combination analysis of hypermethylated Wnt-antagonist family genes as a novel epigenetic biomarker panel for bladder cancer detection. Clin Cancer Res 12: 2109-2116, 2006.
16. Kuphal S, Lodermeyer S, Bataille F, Schuierer M, Hoang BH and Bosserhoff AK: Expression of Dickkopf genes is strongly reduced in malignant melanoma. Oncogene 25: 5027-5036, 2006.
17. Sato H, Suzuki H, Toyota M, Nojima M, Maruyama R, Sasaki S, Takagi H, Sogabe Y, Sasaki Y, Idogawa M, Sonoda T, Mori M, Imai K, Tokino T and Shinomura Y: Frequent epigenetic inactivation of DICKKOPF family genes in human gastrointestinal tumors. Carcinogenesis 28: 2459-2466, 2007.
18. Hoang BH, Kubo T, Healey JH, Yang R, Nathan SS, Kolb EA, Mazza B, Meyers PA and Gorlick R: Dickkopf 3 inhibits invasion and motility of Saos-2 osteosarcoma cells by modulating the Wnt-beta-catenin pathway. Cancer Res 64: 2734-2739, 2004.
19. Abarzua F, Sakaguchi M, Takaishi M, Nasu Y, Kurose K, Ebara S, Miyazaki M, Namba M, Kumon H and Huh NH: Adenovirus-mediated overexpression of REIC/Dkk-3 selectively induces apoptosis in human prostate cancer cells through activation of c-Jun-NH2-kinase. Cancer Res 65: 9617-9622, 2005.
20. Edamura K, Nasu Y, Takaishi M, Kobayashi T, Abarzua F, Sakaguchi M, Kashiwakura Y, Ebara S, Saika T, Watanabe M, Huh NH and Kumon H: Adenovirus-mediated REIC/Dkk-3 gene transfer inhibits tumor growth and metastasis in an orthotopic prostate cancer model. Cancer Gene Ther 14: 765-772, 2007.
21. Veeck J, Bektas N, Hartmann A, Kristiansen G, Heindrichs U, Knüchel R and Dahl E: Wnt signalling in human breast cancer: expression of the putative Wnt inhibitor Dickkopf-3 (DKK3) is frequently suppressed by promoter hypermethylation in mammary tumours. Breast Cancer Res 10: R82, 2008.
22. Licchesi JD, Westra WH, Hooker CM, Machida EO, Baylin SB and Herman JG: Epigenetic alteration of Wnt pathway antagonists in progressive glandular neoplasia of the lung. Carcinogenesis 29: 895-904, 2008.
23. Kawasaki K, Watanabe M, Sakaguchi M, Ogasawara Y, Ochiai K, Nasu Y, Doihara H, Kashiwakura Y, Huh NH, Kumon H and Date H: REIC/Dkk-3 overexpression downregulates P-glycoprotein in multidrug-resistant MCF7/ADR cells and induces apoptosis in breast cancer. Cancer Gene Ther 16: 65-72, 2009.
24. Tsuji T, Miyazaki M, Sakaguchi M, Inoue Y and Namba M: A REIC gene shows down-regulation in human immortalized cells and human tumor-derived cell lines. Biochem Biophys Res Commun 268: 20-24, 2000. (Pubitemid 30116603)
25. Namba M and Tsuji T: Early events during neoplastic transformation of human cells in vitro: Genetic and biological aspects of immortalization. In: Molecular Pathology of Early Cancer, Srivastava S, Henson DE, Gazdar A (eds). IOS Press, Amsterdam, pp27-38, 1999.
26. Monaghan AP, Kioschis P, Wu W, Zuniga A, Bock D, Poustka A, Delius H and Niehrs C: Dickkopf genes are co-ordinately expressed in mesodermal lineages. Mech Dev 87: 45-56, 1999. (Pubitemid 29428085)
27. Bass DA, Parce JW, Dechatelet LR, Szedja P, Seeds MC and Thomas M: Flow cytometric studies of oxidative product formation by neutrophils. A graded response to membrane stimulation. J Immunol 130: 1910-1917, 1983.
28. Tavassoli FA and Devilee P: World Health Organization Classification of Tumours. Pathology and Genetics of Tumours of the Breast and Female Genital Organs. IARC Press, Lyon, 2003.
29. Martindale JL and Holbrook NJ: Cellular response to oxidative stress: signaling for suicide and survival. J Cell Physiol 192: 1-15, 2002.
30. Balaban RS, Nemoto S and Finkel T: Mitochondria, oxidants, and aging. Cell 120: 483-495, 2005.
31. Haupt S, Berger M, Goldberg Z and Haupt Y: Apoptosis - the p53 network. J Cell Sci 116: 4077-4085, 2003.
32. Gupta S: Molecular signaling in death receptor and mitochondrial pathways of apoptosis (Review). Int J Oncol 22: 15-20, 2003.
33. Su TT: Cellular responses to DNA damage: One signal, multiple choices. Ann Rev Genet 40: 187-208, 2006. (Pubitemid 44956784)
34. Thornborrow EC, Patel S, Mastropietro AE, Schwartzfarb EM and Manfredi JJ: A conserved intronic response element mediates direct p53-dependent transcriptional activation of both the human and murine bax genes. Oncogene 21: 990-999, 2002.
35. Skulachev VP: Cytochrome c in the apoptotic and antioxidant cascades. FEBS Lett 423: 275-280, 1998.
36. Adams JM and Cory S: The Bcl-2 protein family: arbiters of cell survival. Science 281: 1322-1326, 1998.
37. Vogt Sionov RV and Haupt Y: The cellular response to p53: the decision between life and death. Oncogene 18: 6145-6157, 1999.
38. Vogelstein B, Lane D and Levine AJ: Surfing the p53 network. Nature 408: 307-310, 2000.
39. Vogt Sionov R, Hayon LI and Haupt Y: The regulation of p53 growth suppression. In: Cell Cycle Checkpoints and Cancer, Blagosklonny MD (ed). Landes Bioscience, Georgetown, TX, pp125, 2001.