A rapid screening method for cell lines producing singly-tagged recombinant proteins using the "TARGET tag" system

Journal of Proteomics

Volumn 73, Issue 9, 2010, Pages 1777-1785

  Tabata, Sanae ^a   Nampo, Maiko ^a   Mihara, Emiko ^a   Tamura Kawakami, Keiko ^a   Fujii, Ikuo ^b   Takagi, Junichi ^a  

^a OSAKA UNIVERSITY   (Japan)

^b Osaka Prefecture University   (Japan)

Author keywords

Affinity tag; Mammalian cell expression; Monoclonal antibody; Phage display; Stable cell line; Surface plasmon resonance

Indexed keywords

RECOMBINANT PROTEIN; HYBRID PROTEIN; MONOCLONAL ANTIBODY; PEPTIDE LIBRARY;

ARTICLE; CELL CLONE; CELL CULTURE; CELL LINE; CONTROLLED STUDY; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN MOTIF; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; SURFACE PLASMON RESONANCE; AFFINITY CHROMATOGRAPHY; BIOSYNTHESIS; CULTURE TECHNIQUE; GENETIC TRANSFECTION; ISOLATION AND PURIFICATION; PEPTIDE LIBRARY; PROCEDURES;

ANTIBODIES, MONOCLONAL; CELL CULTURE TECHNIQUES; CELL LINE; CHROMATOGRAPHY, AFFINITY; HUMANS; PEPTIDE LIBRARY; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; SURFACE PLASMON RESONANCE; TRANSFECTION;

MAMMALIA;

EID: 77954657682     PISSN: 18743919     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jprot.2010.05.012     Document Type: Article

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