Development of an epitope tag for the gentle purification of proteins by immunoaffinity chromatography: Application to epitope-tagged green fluorescent protein

Analytical Biochemistry

Volumn 323, Issue 2, 2003, Pages 171-179

  Thompson, Nancy E ^a   Arthur, Terrance M ^a,b   Burgess, Richard R ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b U S MEAT ANIMAL RESEARCH CENTER   (United States)

Author keywords

Epitope tag; Green fluorescent protein (GFP); Immunoaffinity chromatography; Polyol responsive; Softag1

Indexed keywords

AFFINITY CHROMATOGRAPHY; ALCOHOLS; ANTIGEN-ANTIBODY REACTIONS; ESCHERICHIA COLI; FLUORESCENCE; MONOCLONAL ANTIBODIES; PURIFICATION; RECOMBINANT PROTEINS;

GREEN FLUORESCENT PROTEIN; IMMUNOAFFINITY CHROMATOGRAPHY; LOW MOLECULAR WEIGHT; MONOCLONAL ANTIBODIES (MABS); POLYHYDROXYLATED; POLYOL-RESPONSIVE; PROTEIN COMPLEXES; SOFTAG1;

EPITOPES;

BACTERIAL RNA; EPITOPE; GREEN FLUORESCENT PROTEIN; MONOCLONAL ANTIBODY; POLYOL; RECOMBINANT DNA; RNA POLYMERASE; SOFTAG 1 EPITOPE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANTIGEN ANTIBODY REACTION; ARTICLE; BETA CHAIN; CARBOXY TERMINAL SEQUENCE; COMPLEX FORMATION; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSED SEQUENCE TAG; IMMUNOAFFINITY CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

ESCHERICHIA COLI;

EID: 0345356399     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2003.08.003     Document Type: Article

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