Novel affinity tag system using structurally defined antibody-tag interaction: Application to single-step protein purification

Protein Science

Volumn 17, Issue 12, 2008, Pages 2120-2126

  Nogi, Terukazu ^a   Sangawa, Takeshi ^a   Tabata, Sanae ^a   Nagae, Masamichi ^a   Tamura Kawakami, Keiko ^a   Beppu, Ayako ^a   Hattori, Mitsuharu ^b   Yasui, Norihisa ^a   Takagi, Junichi ^a  

^a OSAKA UNIVERSITY   (Japan)

^b NAGOYA CITY UNIVERSITY   (Japan)

Author keywords

Affinity tag; F spondin; Fab fragment; Monoclonal antibody; Purification; Reelin; ScFv fragment; X ray crystallography

Indexed keywords

MONOCLONAL ANTIBODY;

ARTICLE; BINDING KINETICS; CRYSTAL STRUCTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; SURFACE PLASMON RESONANCE;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; CELL ADHESION MOLECULES, NEURONAL; CELL LINE, TUMOR; CHROMATOGRAPHY, AFFINITY; CRYSTALLOGRAPHY, X-RAY; EPITOPES; EXTRACELLULAR MATRIX PROTEINS; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; KINETICS; MICE; NERVE TISSUE PROTEINS; PEPTIDES; PROTEIN BINDING; PROTEINS; RECOMBINANT FUSION PROTEINS; SERINE ENDOPEPTIDASES; STRUCTURE-ACTIVITY RELATIONSHIP;

MAMMALIA;

EID: 56749168901     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.038299.108     Document Type: Article

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