Overexpression of connexin26 in the basal keratinocytes reduces sensitivity to tumor promoter TPA

Experimental Dermatology

Volumn 19, Issue 7, 2010, Pages 633-640

  Wang, Xiao ^a   Ramirez, Angel ^b   Budunova, Irina ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

^b CIEMAT   (Spain)

Author keywords

Connexin26; Keratinocyte; Proliferation; Protein kinase C; TPA; Transgenic mice

Indexed keywords

CONNEXIN 26; CONNEXIN 43; CYTOKERATIN 5; PHORBOL 13 ACETATE 12 MYRISTATE; PROTEIN KINASE C; TUMOR PROMOTER;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL DIFFERENTIATION; CELL PROLIFERATION; CONTROLLED STUDY; DOWN REGULATION; ENZYME ACTIVITY; ENZYME REGULATION; GENE OVEREXPRESSION; KERATINOCYTE; MOUSE; NONHUMAN; PROTEIN EXPRESSION;

ANIMALS; CARCINOGENS; CATTLE; CELL DIFFERENTIATION; CELL PROLIFERATION; CONNEXINS; GENE EXPRESSION; KERATIN-5; KERATINOCYTES; MICE; MICE, TRANSGENIC; PROMOTER REGIONS, GENETIC; PROTEIN KINASE C; RATS; RECOMBINANT PROTEINS; TETRADECANOYLPHORBOL ACETATE;

EID: 77954655905     PISSN: 09066705     EISSN: 16000625     Source Type: Journal    
DOI: 10.1111/j.1600-0625.2009.01013.x     Document Type: Article

References (41)

1. Mese G, Richard G, White T W. Gap junctions: basic structure and function. J Invest Dermatol 2007, 127:2516-2524.
2. Sohl G, Willecke K. An update on connexin genes and their nomenclature in mouse and man. Cell Commun Adhes 2003, 10:173-80.
3. Gemel J, Lin X, Veenstra R D. N-terminal residues in Cx43 and Cx40 determine physiological properties of gap junction channels, but do not influence heteromeric assembly with each other or with Cx26. J Cell Sci 2006, 119:2258-2268.
4. Gemel J, Valiunas V, Brink P R. Connexin43 and connexin26 form gap junctions, but not heteromeric channels in co-expressing cells. J Cell Sci 2004, 117:2469-2480.
5. Tomasetto C, Neveu M J, Daley J. Specificity of gap junction communication among human mammary cells and connexin transfectants in culture. J Cell Biol 1993, 122:157-167.
6. White T W, Paul D L, Goodenough D A. Functional analysis of selective interactions among rodent connexins. Mol Biol Cell 1995, 6:459-470.
7. Budunova I V, Carbajal S, Slaga T J. The expression of gap junctional proteins during different stages of mouse skin carcinogenesis. Carcinogenesis 1995, 16:2717-2724.
8. Butterweck A, Elfgang C, Willecke K. Differential expression of the gap junction proteins connexin45, -43, -40, -31, and -26 in mouse skin. Eur J Cell Biol 1994, 65:152-163.
9. Kretz M, Maass K, Willecke K. Expression and function of connexins in the epidermis, analyzed with transgenic mouse mutants. Eur J Cell Biol 2004, 83:647-654.
10. Mesnil M, Krutovskikh V, Piccoli C. Negative growth control of HeLa cells by connexin genes: connexin species specificity. Cancer Res 1995, 55:629-639.
11. Budunova I V, Carbajal S, Slaga T J. Effect of diverse tumor promoters on the expression of gap-junctional proteins connexin (Cx)26, Cx31.1, and Cx43 in SENCAR mouse epidermis. Mol Carcinog 1996, 15:202-214.
12. Goliger J A, Paul D L. Wounding alters epidermal connexin expression and gap junction-mediated intercellular communication. Mol Biol Cell 1995, 6:1491-1501.
13. Kamibayashi Y, Oyamada Y, Mori M. Aberrant expression of gap junction proteins (connexins) is associated with tumor progression during multistage mouse skin carcinogenesis in vivo. Carcinogenesis 1995, 16:1287-1297.
14. Labarthe M P, Bosco D, Saurat J H. Upregulation of connexin 26 between keratinocytes of psoriatic lesions. J Invest Dermatol 1998, 111:72-76.
15. Lucke T, Choudhry R, Thom R. Upregulation of connexin 26 is a feature of keratinocyte differentiation in hyperproliferative epidermis, vaginal epithelium, and buccal epithelium. J Invest Dermatol 1999, 112:354-361.
16. Djalilian A R, McGaughey D, Patel S. Connexin 26 regulates epidermal barrier and wound remodeling and promotes psoriasiform response. J Clin Invest 2006, 116:1243-1253.
17. Pointis G, Fiorini C, Gilleron J. Connexins as precocious markers and molecular targets for chemical and pharmacological agents in carcinogenesis. Curr Med Chem 2007, 14:2288-2303.
18. Yamasaki H, Omori Y, Zaidan-Dagli M L. Genetic and epigenetic changes of intercellular communication genes during multistage carcinogenesis. Cancer Detect Prev 1999, 23:273-279.
19. Murillas R, Larcher F, Conti C J. Expression of a dominant negative mutant of epidermal growth factor receptor in the epidermis of transgenic mice elicits striking alterations in hair follicle development and skin structure. Embo J 1995, 14:5216-5223.
20. Budunova I V, Carbajal S, Kang H. Altered glucocorticoid receptor expression and function during mouse skin carcinogenesis. Mol Carcinog 1997, 18:177-185.
21. Kloepper J E, Tiede S, Brinckmann J. Immunophenotyping of the human bulge region: the quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche. Exp Dermatol 2008, 17:592-609.
22. Perez P, Page A, Bravo A. Altered skin development and impaired proliferative and inflammatory responses in transgenic mice overexpressing the glucocorticoid receptor. FASEB J 2001, 15:2030-2032.
23. Kamibayashi Y, Oyamada M, Oyamada Y. Expression of gap junction proteins connexin 26 and 43 is modulated during differentiation of keratinocytes in newborn mouse epidermis. J Invest Dermatol 1993, 101:773-778.
24. Muller-Decker K, Berger I, Ackermann K. Cystic duct dilatations and proliferative epithelial lesions in mouse mammary glands upon keratin 5 promoter-driven overexpression of cyclooxygenase-2. Am J Pathol 2005, 166:575-584.
25. Aldaz C M, Conti C J, Gimenez I B. Cutaneous changes during prolonged application of 12-O-tetradecanoylphorbol-13-acetate on mouse skin and residual effects after cessation of treatment. Cancer Res 1985, 45:2753-2759.
26. Molloy C J, Laskin J D. Specific alterations in keratin biosynthesis in mouse epidermis in vivo and in explant culture following a single exposure to the tumor promoter 12-O-tetradecanoylphorbol-13-acetate. Cancer Res 1987, 47:4674-4680.
27. Slaga T J, DiGiovanni J, Winberg L D. Skin carcinogenesis: characteristics, mechanisms, and prevention. Prog Clin Biol Res 1995, 391:1-20.
28. Hirschi K K, Xu C E, Tsukamoto T. Gap junction genes Cx26 and Cx43 individually suppress the cancer phenotype of human mammary carcinoma cells and restore differentiation potential. Cell Growth Differ 1996, 7:861-870.
29. Momiyama M, Omori Y, Ishizaki Y. Connexin26-mediated gap junctional communication reverses the malignant phenotype of MCF-7 breast cancer cells. Cancer Sci 2003, 94:501-507.
30. Tanaka M, Grossman H B. Connexin 26 gene therapy of human bladder cancer: induction of growth suppression, apoptosis, and synergy with Cisplatin. Hum Gene Ther 2001, 12:2225-2236.
31. Tanaka M, Grossman H B. Connexin 26 induces growth suppression, apoptosis and increased efficacy of doxorubicin in prostate cancer cells. Oncol Rep 2004, 11:537-41.
32. Yano T, Hernandez-Blazquez F J, Omori Y. Reduction of malignant phenotype of HEPG2 cell is associated with the expression of connexin 26 but not connexin 32. Carcinogenesis 2001, 22:1593-1600.
33. Sawey M J, Goldschmidt M H, Risek B. Perturbation in connexin 43 and connexin 26 gap-junction expression in mouse skin hyperplasia and neoplasia. Mol Carcinog 1996, 17:49-61.
34. Garg R, Ramchandani A G, Maru G B. Curcumin decreases 12-O-tetradecanoylphorbol-13-acetate-induced protein kinase C translocation to modulate downstream targets in mouse skin. Carcinogenesis 2008, 29:1249-1257.
35. Cataisson C, Joseloff E, Murillas R. Activation of cutaneous protein kinase C alpha induces keratinocyte apoptosis and intraepidermal inflammation by independent signaling pathways. J Immunol 2003, 171:2703-2713.
36. Cerda S R, Mustafi R, Little H. Protein kinase C delta inhibits Caco-2 cell proliferation by selective changes in cell cycle and cell death regulators. Oncogene 2006, 25:3123-3138.
37. Kazi J U, Soh J W. Isoform-specific translocation of PKC isoforms in NIH3T3 cells by TPA. Biochem Biophys Res Commun 2007, 364:231-237.
38. Bakirtzis G, Choudhry R, Aasen T. Targeted epidermal expression of mutant Connexin 26(D66H) mimics true Vohwinkel syndrome and provides a model for the pathogenesis of dominant connexin disorders. Hum Mol Genet 2003, 12:1737-1744.
39. Yuspa S H, Hennings H, Tucker R W. Signal transduction for proliferation and differentiation in keratinocytes. Ann N Y Acad Sci 1988, 548:191-196.
40. Solan J L, Lampe P D. Connexin phosphorylation as a regulatory event linked to gap juncion channel assembly. Biochim Biophys Acta 2005, 171:154-163.
41. Lima F, Niger C, Hebert C, Stains J P. Connexin43 potentiates osteoblast responsiveness to fibroblast growth factor 2 via a protein kinase C-delta/Runx2-dependent mechanism. Mol Biol Cell 2009, 20:2697-2708. Epub 2009 Apr 1