Gap junction genes Cx26 and Cx43 individually suppress the cancer phenotype of human mammary carcinoma cells and restore differentiation potential

Cell Growth and Differentiation

Volumn 7, Issue 7, 1996, Pages 861-870

  Hirschi, Karen K ^a,b   Xu, Cheng En ^a   Tsukamoto, Tetsuya ^a,c   Sager, Ruth ^a  

^a DANA FARBER CANCER INSTITUTE   (United States)

^b BOSTON CHILDREN S HOSPITAL   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GAP JUNCTION PROTEIN; MATRIGEL;

ARTICLE; BREAST CARCINOMA; CARCINOMA CELL; CELL COMMUNICATION; CELL DIFFERENTIATION; EPITHELIUM CELL; EXTRACELLULAR MATRIX; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MORPHOGENESIS; PHENOTYPE; PRIORITY JOURNAL; RECEPTOR DOWN REGULATION; TRANSCRIPTION REGULATION; TUMOR GROWTH;

BREAST NEOPLASMS; CARCINOMA; CELL DIFFERENTIATION; CONNEXIN 43; CONNEXINS; FEMALE; GAP JUNCTIONS; GENE EXPRESSION REGULATION, NEOPLASTIC; GENE TRANSFER TECHNIQUES; GENES, TUMOR SUPPRESSOR; HUMANS; TUMOR CELLS, CULTURED;

ANIMALIA;

EID: 0029827827     PISSN: 10449523     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (45)

1. Loewenstein, W. R. Junctional intercellular communication and the control of growth. Biochim. Biophys. Acta, 560: 1-65, 1979.
2. Loewenstein, W. R., and Kanno, Y. Intercellular communication and tissue growth: lack of communication between cancer cells. Nature (Lond.), 209: 1248-1249, 1966.
3. Loewenstein, W. R., and Kanno, Y. Intercellular communication and tissue growth: I. Cancerous growth. J. Cell Biol., 33: 225-234, 1967.
4. Stoker, M. G. P. Transfer of growth inhibition between normal and virus-transformed cells: autoradiographic studies using marked cells. J. Cell Sci., 2: 293-304, 1967.
5. Mehta. P. P., Bertram, J. S., and Loewenstein, W. R. Growth inhibition of transformed cells correlates with their junctional communication with normal cells. Cell, 44: 187-196, 1986.
6. Zhu, D., Caveny, S., Kidder, G. M., and Naus, C. G. Transfection of C6 glioma cells with connexin43 cDNA: analysis of expression, intercellular coupling, and cell proliferation. Proc. Natl. Acad. Sci. USA, 88: 1883-1887, 1991.
7. Mehta, P., Holtz-Wagenblatt, A., Rose, B., Shalloway, D., and Loewenstein, W. Incorporation of the gene for a cell-cell channel protein into transformed cells leads to normalization of growth. J. Membr. Biol., 124: 207-225, 1991.
8. Naus, C. C. G., Elisevich, K., Zhu, D., Belliveau, D. J., and Del Maestro, R. F. In vivo growth of C6 glioma cells transfected with connexin43 cDNA. Cancer Res., 52: 4208-4213, 1992.
9. Eghbali, B., Kessler, J. A., Reid, L. M., Roy, C., and Spray, D. C. Involvement of gap junctions in tumorigenesis: transfection of tumor cells with connexin32 cDNA retards growth in vivo. Proc. Natl. Acad. Sci. USA, 88: 10701-10705, 1991.
10. Chen, S., Pelletier, D. B., Ao, P., and Boynton, A. L. Connexin43 reverses the phenotype of transformed cells and alters their expression of cyclin/cyclin-dependent kinases. Cell Growth & Differ., 6: 681-690, 1995.
11. Lee, S. W., Paul, D. L., Keyomarsi, K., and Sager, R. Transcriptional down-regulation of gap junction proteins blocks junctional communication in human mammary tumor cell lines. J. Cell Biol., 118: 1213-1221, 1992.
12. Tomasetto, C., Neveu, M. J., Daley, J., Horan, P. K., and Sager, R. Specificity of gap junction communication among human mammary cells and connexin transfectants in culture. J. Cell Biol., 122: 157-167, 1993.
13. Wilgenbus, K. K., Kirkpatrick, C. J., Knuechel, R., Willecke, K., and Traub, O. Expression of Cx26, Cx32, and Cx43 gap junction proteins in normal and neoplastic human tissues. Int. J. Cancer, 57: 522-529, 1992.
14. Krutovskikh, V., Mazzoleni, G., Mironov, N., Omori, Y., Agueloni, A-M., Mesnil, M., Berger, F., Partensky, C., and Yamasaki, H. Altered homologous and heterologous gap-junctional intercellular communication in primary human liver tumors associated with aberrant protein localization but not gene mutation of connexin32. Int. J. Cancer, 56: 87-94, 1994.
15. Oyamada, Y., Oyamada, M., Fusco, A., and Yamasaki, H. Aberrant expression, function, and localization of connexins in human esophageal carcinoma cell lines with different degrees of tumorigenicity. Can. Res. Clin. Oncol., 120: 445-453, 1994.
16. Price, J. E., Polyzos, A., Zhang, R. D., and Daniels, L. M. Tumorigenecity and metastases of human breast carcinoma cell lines in nude mice. Cancer Res., 50: 717-721, 1990.
17. Streuli, C. H., Bailey, N., and Bissell, M. J. Control of mammary epithelial differentiation: basement membrane induces tissue-specific gene expression in the absence of cell-cell interaction and morphological polarity. J. Cell Biol., 115: 1383-1395, 1991.
18. Petersen, O. W., Rønnov-Jessen, L., Howlett, A. R., and Bissell, M. J. Interaction with basement membrane serves to rapidly distinguish growth and differentiation pattern of normal and malignant human breast epithelial cells. Prac. Natl. Acad. Sci. USA, 89: 9064-9068, 1992.
19. Sager, R., Sheng, S., Anisowicz, A., Sotiropoulou, G., Zou, Z., Stenman, G., Swisshelm, K., Chen, Z., Hendrix, M. J. C., Pemberton, P., Rafidi, K., and Ryan, K. RNA genetics of breast cancer: maspin as a paradigm. Cold Spring Harbor Symp. Quant. Biol., 24: 537-546.
20. Musil, L. S., and Goodenough, D. A. Gap junctional intercellular communication and the regulation of connexin expression and function. Curr. Opin. Cell Biol., 2: 875-880, 1990.
21. Musil, L. S., and Goodenough, D. A. Biochemical analysis of connexin43 intracellular transport, phosphorylation, and assembly into gapjunctional plaques. J. Cell Biol., 115: 1357-1374, 1991.
22. 2+. Eur. J. Cell Biol., 57: 40-50, 1992.
23. Moreno, A. P., Sáez, J. C., Fishman, G. I., and Spray, D. C. Human Cx43 gap junction channels: regulation of unitary conductances by phospriorylation. Circ. Res., 74: 1050-1057, 1994.
24. Paludan, K., Duch, M., Jørgensen, P., Kjeldgaard, N. O., and Pedersen, F. S. Graduated resistance to G418 leads to differential selection of cultured mammalian cells expressing the neo gene. Gene (Amst.), 85: 421-426, 1989.
25. Shimohama, S., Rosenberg, M. B., Fagan, A. M., Wolff, J. A., Short, M. P., Breakefield, X. O., Friedmann, T., and Gage, F. H. Grafting genetically modified cells into the rat brain: characteristics of E. coli β-galactosidase as a reporter gene. Mol. Brain Res., 5: 271-278, 1989.
26. Spray, D. C., and Burt, J. M. Structure-activity relations of the cardiac gap junction channel. Am. J. Physiol., 258: C195-C205, 1990.
27. Willecke, K., Hennemann, H., Dahl, E., Jungbluth, S., and Heynkes, R. The diversity of connexin genes encoding gap junctional proteins. Eur. J. Cell Biol., 56: 1-7, 1991.
28. 2+/calmodulin-dependent protein kinase II. Eur. J. Biochem., 192: 263-273, 1990.
29. Bradshaw, S. L., Naus, C. C. G., Zhu, D., Kidder, G. M., D'Ercole, A. J., and Han, V. K. M. Alterations in the synthesis of insulin-like growth factor binding proteins and insulin-like growth factors in rat C6 glioma cells transfected with a gap junction connexin43 cDNA. Regul. Pept., 48: 99-112, 1993.
30. Guthrie, S. C., and Gilula, N. B. Gap junctional communication and development. Trends Neurosci., 12: 12-16, 1989.
31. Reaume, A. G., de Sousa, P. A., Kulkarni, S., Langille, B. L., Zhu, D., Davies, T. C., Juneja, S. C., Kidder, G. M., and Rossant, J. Cardiac malformation in neonatal mice lacking connexin43. Science (Washington DC), 267: 1831-1834, 1995.
32. Veenstra, R. D., Wang, H-Z., Beyer, E. C., Ramanan, S. V., and Brink, P. R. Connexin37 forms high conductance gap junction channels with subconductance state activity and selective dye and ionic permeabilities. Biophys J., 66: 1915-1928, 1994.
33. Veenstra, R. D., Wang, H-Z., Beyer, E. C., and Brink, P. R. Selective dye and ionic permeability of gap junction channels formed by connexin45. Circ. Res., 75: 483-490, 1994.
34. Elfgang, C., Eckert, R., Fraté-Lichtenberg, H., Butterweck, A., Traub, O., Klein, R. A., HÜlser, D. F., and Willecke, K. Specific permeability and selective formation of gap junction channels in connexin-transfected HeLa cells. J. Cell Biol., 129: 805-817, 1995.
35. Little, T. L., Xia, J., and Duling, B. R. Dye tracers define differential endothelial and smooth muscle coupling patterns within the arteriolar wall. Cir. Res., 76: 498-504, 1995.
36. Sachs, L. Cell differentiation and bypassing of genetic defects in the suppression of malignancy. Cancer Res., 47: 1981-1986, 1987.
37. Band, V., and Sager, R. Distinctive traits of normal and tumor-derived human mammary epithelial cells expressed in a medium that supports long-term growth of both cell types. Proc. Natl. Acad. Sci. USA, 86; 1249-1253, 1989.
38. Band, V., Zajchowski, D., Stenman, G., Morton, C. C., Kulesa, V., Connolly, J., and Sager, R. A newly established metastatic breast tumor cell line with integrated amplified copies of erbB2 and double minute chromosomes. Genes Chromosomes & Cancer, 1: 48-58, 1990.
39. Fishman, G. I., Spray, D. C., and Leinwand, L. A. Molecular characterization and functional expression of the human cardiac gap junction channel. J. Cell Biol., 111: 589-598, 1990.
40. Li, C. J., Wang, C., and Pardee, A. B. Camptothecin inhibits Tatmediated transactivation of type 1 human immunodeficiency virus. J. Biol. Chem., 269: 7051-7054, 1994.
41. Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J., and Rutler, W. J. Isolation of biologically active RNA from sources enriched in ribonuclease. Biochemistry, 18: 5294-5299, 1979.
42. Feinberg, A. P., and Vogelstein, B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem., 137: 266-267, 1983.
43. Masiakowski, P., Breathnach, R., Bloch, J., Gannon, F., Krust, A., and Chambon, P. Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line. Nucleic Acids Res., 10: 7895-7903, 1982.
44. Ruch, R. J., Bonney, W. J., Sigler, K., Guan, X., Matesic, D., Schafer, L. D., Dupont, E., and Trosko, J. E. Loss of gap junctions from DDT-treated rat liver epithelial cells. Carcinogenesis (Lond.), 15: 301-306, 1994.
45. El-Fouly, M. H., Trosko, J. E., and Chang, C. C. Scrape loading and dye transfer: a rapid and simple technique to study gap junctional intercellular communication. Exp. Cell Res., 168: 422-430, 1987.