Early detection of Aspergillus carbonarius and A. niger on table grapes: A tool for quality improvement

Food Additives and Contaminants - Part A

Volumn 27, Issue 9, 2010, Pages 1285-1293

  Ayoub, F ^a   Reverberi, M ^b   Ricelli, A ^c   D'Onghia, A M ^a   Yaseen, T ^a  

^a ISTITUTO AGRONOMICO MEDITERRANEO   (Italy)

^b SAPIENZA UNIVERSITY OF ROME   (Italy)

^c CNR Consiglio Nazionale delle Ricerche   (Italy)

Author keywords

Fruit; Fungi; High performance liquid chromatography (HPLC); Molecular biology; Mycotoxins; Ochratoxin a; Polymerase chain reaction (PCR)

Indexed keywords

OCHRATOXIN; POLYKETIDE SYNTHASE; PRIMER DNA;

ARTICLE; ASPERGILLUS CARBONARIUS; ASPERGILLUS NIGER; FOOD CONTAMINATION; FUNGAL DETECTION; FUNGAL STRAIN; GRAPE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; AGAR GEL ELECTROPHORESIS; ASPERGILLUS; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; MICROBIOLOGY; NUCLEOTIDE SEQUENCE; QUALITY CONTROL; VITIS;

ASPERGILLUS CARBONARIUS; FUNGI; VITACEAE;

ASPERGILLUS; BASE SEQUENCE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA PRIMERS; ELECTROPHORESIS, AGAR GEL; FOOD CONTAMINATION; POLYMERASE CHAIN REACTION; QUALITY CONTROL; VITIS;

EID: 77954645678     PISSN: 19440049     EISSN: 14645122     Source Type: Journal    
DOI: 10.1080/19440049.2010.489576     Document Type: Article

References (26)

1. Ailsa DH, Su-lin LL, Benozir AK, Robert WE, Eileen SS. 2007. Fungi and mycotoxins in vineyards and grape products. Int J Food Microbiol. 119:84-88.
2. Atoui A. 2006. Approche de la mycotoxinogenese chez Aspergillus ochraceus et Aspergillus carbonarius: etudes moleculaire et physiologique [PhD thesis]. [Tolouse (France)]: ENSAT.
3. Atoui A, Mathieu F, Lebrihi A. 2006. Targeting a polyketide synthase gene for Aspergillus carbonarius quantification and ochratoxin A assessment in grapes using real-time PCR. Int J Food Microbiol. 115:313-318.
4. Battaglia R, Hatzold T, Kroes R. 1996. Occurrence and significance of ochratoxin A in food. Food Addit Contam. 13:1-3.
5. Battilani P, Pietri A, Bertuzzi T, Languasco L, Giorni P, Kozakiewicz Z. 2003. Occurrence of ochratoxin A-producing fungi in grapes grown in Italy. J Food Protect. 66:633-636.
6. Battilani P, Logrieco A, Giorni P, Cozzi G, Bertuzzi T, Pietri A. 2004. Ochratoxin A production by Aspergillus carbonarius on some grape varieties grown in Italy. J Sci Food Agricult. 84(13):1736-1740.
7. Belli N. 2006. Ochratoxin A and ochratoxigenic moulds in grapes, must and wine. Ecophysiological studies [PhD thesis]. [Lleida (Spain)]: Universitat de Lleida.
8. Bluhm BH, Flaherty JE, Cousin MA, Woloshuk CP. 2002. Multiplex polymerase chain reaction assay for the differ- ential detection of trichothecene- and fumonisin-producing species of Fusarium in cornmeal. J Food Protect. 65:1955-1961.
9. Ginzinger DG. 2002. Gene quantification using real-time quantitative PCR: an emerging technology hits the main stream. Exp Hematol. 30:503-512.
10. Gonzalez-Salgado A, Patino B, Gil-Serna J, Vazquez C, Gonzalez-Jaen MT. 2009. Specific detection of Aspergillus carbonarius by SYBR Green and TaqMans quantitative PCR assays based on the multicopy ITS2 region of the rRNA gene. FEMS Microbiol Lett. 295:57-66.
11. Guzev L, Danshin A, Ziv S, Lichter A. 2006. Occurrence of ochratoxin A producing fungi in wine and table grapes in Israel. Int J Food Microbiol. 111:S67-S71.
12. Leong SL, Hocking AD, Scott ES. 2006. Survival and growth of Aspergillus carbonarius on wine grapes before harvest. Int J Food Microbiol. 111(1):S83-S87.
13. Lie YS, Petropoulos CJ. 1998. Advances in quantitative PCR technology: 5̀ nuclease assays. Curr Opin Biotechnol. 9:43-48.
14. Mahuku GS, Platt HW. 2002. Quantifying Verticillium dahliae in soils collected from potato fields using a competitive PCR assay. Am J Potato Res. 97:107-117.
15. Parenicova L, Skouboe P, Samson RA, Rossen L, Visser J. 2000. Integration of modern taxonomic methods of Penicillium and Aspergillus classification. In: Samson RA Pitt JI, editors. Molecular tools for the classification of black Aspergilli. Amsterdam (the Netherlands): Hardwood, p. 413-424.
16. Patino B, Gonzalez-Salgado A, Gonzalez-Jaen MT, Vazquez C. 2005. PCR detection assays for the ochratoxin producing Aspergillus carbonarius and Aspergillus ochraceus species. Int J Food Microbiol. 104: 207-214.
17. Pitt JI, Hocking AD. 1996. Current knowledge of fungi and mycotoxins associated with food commodities in Southeast Asia. Paper presented at: 17th Asean Technical Seminar on Grain Postharvest Technology - Mycotoxin Contamination in Grains; Lumut (Malaysia), Book Series: Aciar Technical Reports No. 37. p.5-10.
18. Raper KB, Fennell DI. 1965. The genus Aspergillus. New York, NY: Williams & Wilkins.
19. Schmittgen TD. 2001. Real-time quantitative PCR. Methods. 25:383-385.
20. Singh RP, Singh US. 1995. Polymerase chain reaction technology in plant pathology. In: Hadidi A, Levy L, Podleckis EV, editors. Molecular methods in plant pathology. London (UK): CRC Press, p. 167-187.
21. Taylor JW, Geiser DM, Burt A, Koufopanou V. 1999. The evolutionary biology and population genetics underlying fungal strain typing. Clinical Microbiol Rev. 12:126-146.
22. Van der Merwe KJ, Steyn PS, Fourie L. 1965. Ochratoxin A,a toxic metabolite produced by Aspergillus ochraceus. Wilh., Nature. 265:1112-1113.
23. Varga J, Kozakiewicz Z. 2006. Ochratoxin A in grapes and grape derived products. Trends Food Sci Technol. 17:72-81.
24. Yaseen T, Ayoub F, Ricelli A. 2009. Partial polyketide synthases sequence of A. niger and specific PCR based detection. Paper presented at: ISM Conference 2009. Proceedings of the ISM Conference Tulln/Vienna; Austria.
25. Yaseen T, Ippolito A, Faretra F, Casulli F. 2004. Molecular diagnosis and biological control of Phytophthora-citrus root rot [PhD thesis]. [Bari (Italy)]: University of Bari.
26. Zhao J, Kong F, Li R, Wang X, Wan Z, Wang D. 2001. Identification of Aspergillus fumigatus and related species by nested PCR targeting ribosomal DNA internal transcribed spacer regions. J Clinical Microbiol. 39:2261-2266.