Critical parameters in blood processing for T-cell assays: Validation on ELISpot and tetramer platforms

Journal of Immunological Methods

Volumn 359, Issue 1-2, 2010, Pages 28-36

  Afonso, Georgia ^a,b   Scotto, Matthieu ^a,b   Renand, Amédée ^c   Arvastsson, Jeanette ^d   Vassilieff, Dominique ^e   Cilio, Corrado M ^d   Mallone, Roberto ^a,b  

^a HÔPITAL SAINT VINCENT DE PAUL   (France)

^b UNIVERSITÉ PARIS DESCARTES   (France)

^c HÔPITAL NECKER ENFANTS MALADES   (France)

^d LUND UNIVERSITY   (Sweden)

^e Hematology Unit ^*  (France)

Author keywords

ELISpot; Peripheral blood mononuclear cells; T cell; Tetramer

Indexed keywords

GAMMA INTERFERON; HLA ANTIGEN; POLYMER; TETRAMER;

ADULT; ARTICLE; BLOOD; BLOOD ANALYSIS; BLOOD CELL COUNT; BLOOD SAMPLING; BLOOD STORAGE; CELL ASSAY; CELL SEPARATION; CONTROLLED STUDY; DENSITY GRADIENT CENTRIFUGATION; ENZYME LINKED IMMUNOSPOT ASSAY; FEMALE; GRANULOCYTE; HEMODILUTION; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; LEUKOCYTE; MALE; NORMAL HUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; SERUM; SYRINGE; T LYMPHOCYTE; TUBE REMOVAL; VACUUM; VALIDATION PROCESS;

ADULT; BLOOD PRESERVATION; BLOOD SPECIMEN COLLECTION; CELL SEPARATION; CENTRIFUGATION, DENSITY GRADIENT; FEMALE; GRANULOCYTES; HLA-A2 ANTIGEN; HUMANS; IMMUNOENZYME TECHNIQUES; INTERFERON-GAMMA; LEUKOCYTE COUNT; LEUKOCYTES, MONONUCLEAR; MALE; T-LYMPHOCYTES;

EID: 77954384687     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2010.05.005     Document Type: Article

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