Rapid identification and sorting of viable virus-reactive CD4+ and CD8+ T cells based on antigen-triggered CD137 expression

Journal of Immunological Methods

Volumn 339, Issue 1, 2008, Pages 23-37

  Wehler, Thomas C ^a   Karg, Michael ^a   Distler, Eva ^a   Konur, Abdo ^a   Nonn, Marion ^a   Meyer, Ralf G ^a   Huber, Christoph ^a   Hartwig, Udo F ^a   Herr, Wolfgang ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

CD137; CD4+ T cells; CD8+ T cells; Cell sorting; T cell monitoring

Indexed keywords

CD137 ANTIGEN; CD40 LIGAND MONOCLONAL ANTIBODY; CD69 ANTIGEN; CD8 ANTIGEN; GAMMA INTERFERON; INTERLEUKIN 2 RECEPTOR ALPHA; MONOCLONAL ANTIBODY; PEPTIDE;

ANTIGEN EXPRESSION; ARTICLE; CD4+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; CELL ISOLATION; CELL SELECTION; CELL STIMULATION; CELL SURFACE; CELL VIABILITY; CONTROLLED STUDY; CYTOKINE RELEASE; CYTOMEGALOVIRUS; EPSTEIN BARR VIRUS; FLOW CYTOMETRY; IN VITRO STUDY; MEMORY T LYMPHOCYTE; NONHUMAN; PRIORITY JOURNAL; T LYMPHOCYTE ACTIVATION;

ADOPTIVE TRANSFER; ANTIGENS, CD137; ANTIGENS, VIRAL; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; CYTOMEGALOVIRUS; GENE EXPRESSION REGULATION; HERPESVIRUS 4, HUMAN; HUMANS; IMMUNOMAGNETIC SEPARATION; INTERFERON-GAMMA; K562 CELLS; LYMPHOCYTE ACTIVATION; PHOSPHOPROTEINS; VIRAL MATRIX PROTEINS;

HUMAN HERPESVIRUS 4;

EID: 53049109038     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2008.07.017     Document Type: Article

References (42)

1. Altman J.D., Moss P.A., Goulder P.J., Barouch D.H., McHeyzer-Williams M.G., Bell J.I., McMichael A.J., and Davis M.M. Phenotypic analysis of antigen-specific T lymphocytes. Science 274 (1996) 94
2. Becker C., Pohla H., Frankenberger B., Schüler T., Assenmacher M., Schendel D.J., and Blankenstein T. Adoptive tumor therapy with T lymphocytes enriched through an IFN-gamma capture assay. Nat. Med. 7 (2001) 1159
3. Betts M.R., Brenchley J.M., Price D.A., De Rosa S.C., Douek D.C., Roederer M., and Koup R.A. Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation. J. Immunol. Methods 281 (2003) 65
4. Britten C.M., Meyer R.G., Kreer T., Drexler I., Wölfel T., and Herr W. The use of HLA-A*0201-transfected K562 as standard antigen-presenting cells for CD8+ T lymphocytes in IFN-γ ELISPOT assays. J. Immunol. Methods 259 (2002) 95
5. Brosterhus H., Brings S., Leyendeckers H., Manz R.A., Miltenyi S., Radbruch A., Assenmacher M., and Schmitz J. Enrichment and detection of live antigen-specific CD4(+) and CD8(+) T cells based on cytokine secretion. Eur. J. Immunol. 29 (1999) 4053
6. Cannons J.L., Lau P., Ghumman B., DeBenedette M.A., Yagita H., Okumura K., and Watts T.H. 4-1BB ligand induces cell division, sustains survival, and enhances effector function of CD4 and CD8 T cells with similar efficacy. J. Immunol. 167 (2001) 1313
7. Chalandon Y., Degermann S., Villard J., Arlettaz L., Kaiser L., Vischer S., Walter S., Heemskerk M.H., van Lier R.A., Helg C., Chapuis B., and Roosnek E. Pretransplantation CMV-specific T cells protect recipients of T-cell-depleted grafts against CMV-related complications. Blood 107 (2006) 389
8. Chattopadhyay P.K., Yu J., and Roederer M. A live-cell assay to detect antigen-specific CD4+ T cells with diverse cytokine profiles. Nat. Med. 11 (2005) 1113
9. Croft M. Co-stimulatory members of the TNFR family: keys to effective T-cell immunity. Nat. Rev. Immunol. 3 (2003) 609
10. Czerkinsky C., Andersson G., Ekre H.P., Nilsson L.A., Klareskog L., and Ouchterlony O. Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells. J. Immunol. Methods 110 (1988) 29
11. Diamond D.J., York J., Sun J.Y., Wright C.L., and Forman S.J. Development of a candidate HLAA*0201 restricted peptide-based vaccine against human cytomegalovirus infection. Blood. 90 (1997) 1751
12. Einsele H., Roosnek E., Rufer N., Sinzger C., Riegler S., Löffler J., Grigoleit U., Moris A., Rammensee H.G., Kanz L., Kleihauer A., Frank F., Jahn G., and Hebart H. Infusion of cytomegalovirus (CMV)-specific T cells for the treatment of CMV infection not responding to antiviral chemotherapy. Blood 99 (2002) 3916
13. Frentsch M., Arbach O., Kirchhoff D., Moewes B., Worm M., Rothe M., Scheffold A., and Thiel A. Direct access to CD4+ T cells specific for defined antigens according to CD154 expression. Nat. Med. 11 (2005) 1118
14. Hackett C.J., and Sharma O.K. Frontiers in peptide-MHC class II multimer technology. Nat. Immunol. 3 (2002) 887
15. Hammer M.H., Meyer S., Brestrich G., Moosmann A., Kern F., Tesfa L., Babel N., Mittenzweig A., Rooney C.M., Hammerschmidt W., Volk H.D., and Reinke P. HLA type-independent generation of antigen-specific T cells for adoptive immunotherapy. Eur. J. Immunol. 35 (2005) 2250
16. Harari A., Zimmerli S.C., and Pantaleo G. Cytomegalovirus (CMV)-specific cellular immune responses. Hum. Immunol. 65 (2004) 500
17. Hengel H., Lucin P., Jonjic S., Ruppert T., and Koszinowski U.H. Restoration of cytomegalovirus antigen presentation by gamma interferon combats viral escape. J. Virol. 68 (1994) 289
18. Herr W., Schneider J., Lohse A.W., Meyer zum Buschenfelde K.H., and Wolfel T. Detection and quantification of blood-derived CD8+ T lymphocytes secreting tumor necrosis factor alpha in response to HLA-A2.1-binding melanoma and viral peptide antigens. J. Immunol. Methods 191 (1996) 131
19. Heslop H.E., Ng C.Y., Li C., Smith C.A., Loftin S.K., Krance R.A., Brenner M.K., and Rooney C.M. Long-term restoration of immunity against Epstein-Barr virus infection by adoptive transfer of gene-modified virus-specific T lymphocytes. Nat. Med. 2 (1996) 551
20. Hviid L., Felsing A., and Theander T.G. Kinetics of human T-cell expression of LFA-1, IL-2 receptor, and ICAM-1 following antigenic stimulation in vitro. J. Clin. Lab. Immunol. 40 (1993) 163
21. June C.H. Principles of adoptive T cell cancer therapy. J. Clin. Invest. 117 (2007) 1204
22. Jung T., Schauer U., Heusser C., Neumann C., and Rieger C. Detection of intracellular cytokines by flow cytometry. J. Immunol. Methods 159 (1993) 197
23. Kern F., Bunde T., Faulhaber N., Kiecker F., Khatamzas E., Rudawski I.M., Pruss A., Gratama J.W., Volkmer-Engert R., Ewert R., Reinke P., Volk H.D., and Picker L.J. Cytomegalovirus (CMV) phosphoprotein 65 makes a large contribution to shaping the T cell repertoire in CMV-exposed individuals. J. Infect. Dis. 185 (2002) 1709
24. Kern F., LiPira G., Gratama J.W., Manca F., and Roederer M. Measuring Ag-specific immune responses: understanding immunopathogenesis and improving diagnostics in infectious disease, autoimmunity and cancer. Trends Immunol. 26 (2005) 477
25. Kwon B.S., and Weissman S.M. cDNA sequences of two inducible T-cell genes. Proc. Natl. Acad. Sci. 86 (1989) 1963
26. Lanzavecchia A., and Sallusto F. Understanding the generation and function of memory T cell subsets. Curr. Opin. Immunol. 17 (2005) 326
27. Mahnke Y.D., Speiser D., Luescher I.F., Cerottini J.C., and Romero P. Recent advances in tumour antigen-specific therapy: in vivo veritas. Int. J. Cancer 113 (2005) 173
28. Manz R., Assenmacher M., Pfluger E., Miltenyi S., and Radbruch A. Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix. Proc. Natl. Acad. Sci. 92 (1995) 1921
29. Mardiney M., Brown M.R., and Fleisher T.A. Measurement of T-cell CD69 expression: a rapid and efficient means to assess mitogen- or antigen-induced proliferative capacity in normals. Cytometry 26 (1996) 305
30. Meyer R.G., Britten C.M., Wehler D., Bender K., Hess G., Konur A., Hartwig U.F., Wehler T.C., Ullmann A.J., Gentilini C., Uharek L., Huber C., Kolbe K., and Herr W. Prophylactic transfer of CD8-depleted donor lymphocytes after T-cell depleted reduced-intensity transplantation. Blood 109 (2007) 374
31. Picker L.J., Singh M.K., Zdraveski Z., Treer J.R., Waldrop S.L., Bergstresser P.R., and Maino V.C. Direct demonstration of cytokine synthesis heterogeneity among human, memory/effector T cells by flow cytometry. Blood 86 (1995) 1408
32. Rauser G., Einsele H., Sinzger C., Wernet D., Kuntz G., Assenmacher M., Campbell J.D., and Topp M.S. Rapid generation of combined CMV-specific CD4+ and CD8+ T-cell lines for adoptive transfer into recipients of allogeneic stem cell transplants. Blood 103 (2004) 3565
33. Reddy M., Eirikis E., Davis C., Davis H.M., and Prabhakar U. Comparative analysis of lymphocyte activation marker expression and cytokine secretion profile in stimulated human peripheral blood mononuclear cell cultures: an in vitro model to monitor cellular immune function. J. Immunol. Methods 293 (2004) 127
34. Sallusto F., Lenig D., Forster R., Lipp M., and Lanzavecchia A. Two subsets of memory T lymphocytes with distinct homing potentials and effector functions. Nature 401 (1999) 708
35. Schwarz H., Valbracht J., Tuckwell J., von Kempis J., and Lotz M. ILA, the human 4-1BB homologue, is inducible in lymphoid and other cell lineages. Blood 85 (1995) 1043
36. Steven N.M., Annels N.E., Kumar A., Leese A.M., Kurilla M.G., and Rickinson A.B. Immediate early and early lytic cycle proteins are frequent targets of the Epstein-Barr virus-induced cytotoxic T cell response. J. Exp. Med. 185 (1997) 1605
37. Waldrop S.L., Pitcher C.J., Peterson D.M., Maino V.C., and Picker L.J. Determination of antigen-specific memory/effector CD4+ T cell frequencies by flow cytometry: evidence for a novel, antigen-specific homeostatic mechanism in HIV-associated immunodeficiency. J. Clin. Invest. 99 (1997) 1739
38. Wehler T.C., Nonn M., Brandt B., Britten C.M., Grone M., Todorova M., Link I., Khan S.A., Meyer R.G., Huber C., Hartwig U.F., and Herr W. Targeting the activation-induced antigen CD137 can selectively deplete alloreactive T cells from antileukemic and antitumor donor T-cell lines. Blood 109 (2007) 365
39. Williams M.A., and Bevan M.J. Effector and memory CTL differentiation. Annu. Rev. Immunol. 25 (2007) 171
40. Wills M.R., Carmichael A.J., Mynard K., Jin X., Weekes M.P., Plachter B., and Sissons J.G. The human cytotoxic T-lymphocyte (CTL) response to cytomegalovirus is dominated by structural protein pp65: frequency, specificity, and T-cell receptor usage of pp65-specific CTL. J. Virol. 70 (1996) 7569
41. Wolfl M., Kuball J., Ho W.Y., Nguyen H., Manley T.J., Bleakley M., and Greenberg P.D. Activation-induced expression of CD137 permits detection, isolation, and expansion of the full repertoire of CD8+ T cells responding to antigen without requiring knowledge of epitope specificities. Blood 110 (2007) 201
42. Young H.A., and Hardy K.J. Role of interferon-gamma in immune cell regulation. J. Leukoc. Biol. 58 (1995) 378