High-level expression of an aspergillus niger endo-β-1,4-glucanase in pichia pastoris through gene codon optimization and synthesis

Journal of Microbiology and Biotechnology

Volumn 20, Issue 3, 2010, Pages 467-473

  Zhao, Shumiao ^a   Huang, Jun ^a   Zhang, Changyi ^a   Deng, Ling ^a   Hu, Nan ^b   Liang, Yunxiang ^a  

^a HUAZHONG AGRICULTURAL UNIVERSITY   (China)

^b NANJING TECH UNIVERSITY   (China)

Author keywords

4 glucanase; Aspergillus niger; Codon optimization; Endo 1; Expression; Pichia pastoris

Indexed keywords

CARBOXYMETHYLCELLULOSE; CELLULASE; OLIGONUCLEOTIDE; RECOMBINANT PROTEIN;

ARTICLE; ASPERGILLUS NIGER; BIOTRANSFORMATION; CODON USAGE; CONTROLLED STUDY; DNA CONTENT; DNA SYNTHESIS; ELECTROPORATION; ENZYME ACTIVITY; ENZYME SUBSTRATE; FUNGAL STRAIN; GENE EXPRESSION; GENE INSERTION; MOLECULAR WEIGHT; NONHUMAN; PH; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROCESS OPTIMIZATION; TEMPERATURE SENSITIVITY;

AMINO ACID SEQUENCE; ASPERGILLUS NIGER; BASE SEQUENCE; BETA-GLUCANS; CELLULASE; CODON; DNA; ENZYME STABILITY; FERMENTATION; MOLECULAR SEQUENCE DATA; PICHIA; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; TRANSFORMATION, GENETIC;

ASPERGILLUS NIGER; HORDEUM; PICHIA PASTORIS;

EID: 77954281012     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: None     Document Type: Article

References (36)

1. Ajithkumar, A., R. Andersson, M. Siika-aho, M. Tenkanen, and P. Aman. 2006. Isolation of cellotriosyl blocks from barley β- glucan with endo-1,4-β-glucanase from Trichoderma reesei. Carbohyd. Polym. 64: 233-238.
2. Bauer, M. W., L. E. Driskill, W. Callen, M. A. Snead, E. J. Mathur, and R. M. Kelly. 1999. An endoglucanase, EglA, from the hyperthermophilic archaeon Pyrococcus furiosus hydrolyzes β-1,4 bonds in mixed-linkage (1→3),(1→4)-β-D-glucans and cellulose. J. Bacteriol. 181: 284-290.
3. Beguin, P. and J. P. Aubert. 1994. The biological degradation of cellulose. FEMS Microbiol. Rev. 13: 25-58.
4. Bhat, M. K. 2004. Cellulases and related enzymes in biotechnology. Biotechnol. Adv. 18: 355-383.
5. Cazemier, A. E., J. C. Verdoes, H. J. M. Op den Camp, J. H. P. Hackstein, and A. J. J. van Ooyen. 1999. A β-1,4 endoglucanaseencoding gene from Cellulomonas pachnodae. Appl. Microbiol. Biotechnol. 52: 232-239.
6. Chang, S. W., G. C. Lee, and J. F. Shaw. 2006. Codon optimization of Candida rugosa lip1 gene for improving expression in Pichia pastoris and biochemical characterization of the purified recombinant LIP1 lipase. J. Agric. Food Chem. 3: 815-822.
7. David, M. H. and L. Jacek. 2002. DNAWorks: An automated method for designing oligonucleotides for PCR-based gene synthesis. Nucleic Acids Res. 30: e43
8. Dienes, D., A. Egyhazi, and K. Reczey. 2004. Treatment of recycled fiber with Trichoderma cellulases. Ind. Crop. Prod. 20: 11-21.
9. Duan, X. Y., S. Y. Liu, W. C. Zhang, Q. X. Zhang, and P. J. Gao. 2004. Volumetric productivity improvement for endoglucanase of Trichoderma pseudokoingii S-38. J. Appl. Microbiol. 96: 772-776.
10. Feng, Y., C. J. Duan, H. Pang, X. C. Mo, C. F. Wu, Y. Yu, et al. 2007. Cloning and identification of novel cellulase genes from uncultured microorganisms in rabbit cecum and characterization of the expressed cellulases. Appl. Microbiol. Biotechnol. 75: 319-328.
11. Hale, R. S. and G. Thompson. 1998. Codon optimization of the gene encoding a domain from human type 1 neurofibromin protein results in a threefold improvement in expression level in Escherichia coli. Protein Expr. Purif. 12: 185-188.
12. Henrissat, B. 1991. A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J. 280: 309-316.
13. Hong, J., T. Hisanori, A. Shunichi, K. Yamamtot, and H. Kumagai. 2001. Cloning of a gene encoding a highly stable endo-β-1,4-glucanase from Aspergillus niger and its expression in yeast. J. Biosci. Bioeng. 92: 434-439.
14. Hu, S., L. Li, J. Qiao, Y. Guo, L. Cheng, and J. Liu. 2006. Codon optimization, expression, and characterization of an internalizing anti-ErbB2 single-chain antibody in Pichia pastoris. Protein Expr. Purif. 47: 249-257.
15. Huang, H., P. Yang, H. Luo, H. Tang, N. Shao, T. Yuan, Y. Wang, Y. Bai, and B. Yao. 2008. High-level expression of a truncated 1,3-1,4-β-D-glucanase from Fibrobacter succinogenes in Pichia pastoris by optimization of codons and fermentation. Appl. Microbiol. Biotechnol. 78: 95-103.
16. Huang, Y., G. Krauss, S. Cottaz, H. Driguez, and G. Lipps. 2005. A highly acid-stable and thermostable endo-β-glucanase from the thermoacidophilic archaeon Sulfolobus solfataricus. Biochem. J. 385: 581-588.
17. Invitrogen: Pichia Expression Kit: A manual of methods for expression of recombinant proteins in Pichia pastoris. Version M. Invitrogen, San Diego, CA, 2002
18. Kitamoto, N., M. Go, T. Shibayama, T. Kimura, Y. Kito, K. Ohmiya, and N. Tsukagoshi. 1996. Molecular cloning, purification and characterization of two endo-1,4-β-glucanases from Aspergillus oryzae KBN616. Appl. Microbiol. Biotechnol. 46: 538-544.
19. Lynd, L. R., J. Paul, P. J. Weimer, W. H. van Zyl, and I. S. Pretorius. 2002. Microbial cellulose utilization: Fundamentals and biotechnology. Microbiol. Mol. Biol. Rev. 66: 506-577.
20. Macauley-Patrick, S., M. L. Fazenda, B. McNeil, and L.M. Harvey. 2005. Heterologous protein production using the Pichia pastoris expression system. Yeast 22: 249-270.
21. Miller, G. L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-428.
22. Nakamura, Y., T. Gojobori, and T. Ikemura. 1999. Codon usage tabulated from the international DNA sequence database; its status 1999. Nucleic Acids Res. 27: 292.
23. Nikolay, S. O., J. S. Willem, and A. J. Maarten. 2002. Optimization of the expression of equistatin in Pichia pastoris. Protein Expr. Purif. 24: 18-24.
24. Ooi, T., A. Shinmyo, H. Okada, S. Murao, T. Kawaguchi, and M. Arai. 1990. Complete nucleotide sequence of a gene coding for Aspergillus aculeatus cellulase (F1-CMCase). Nucleic Acids Res. 18: 5884.
25. Palomer, X., E. Dominguez-Puigjaner, M. Vendrell, and I. Llop-Tous. 2004. Study of the strawberry Cel1 endo-(1,4)-glucanase protein accumulation and characterization of its in vitro activity by heterologous expression in Pichia pastoris. Plant Sci. 167: 509-518.
26. Peilong, Y., P. Shi, Y. Wang, Y. Bai, K. Meng, H. Luo, T. Yuan, and B. Yao. 2007. Cloning and overexpression of a Paenibacillus β-glucanase in Pichia pastoris: Purification and characterization of the recombinant enzyme. J. Microbiol. Biotechnol. 17: 58-66.
27. Rose, S. H. and W. H. van Zyl. 2002. Constitutive expression of the Trichoderma reesei β-1,4-xylanase gene (xyn2) and the β- 1,4-endoglucanase gene (egI) in Aspergillus niger in molasses and defined glucose media. Appl. Microbiol. Biotechnol. 58: 461-468.
28. Sakamoto, S., G. Tamura, K. Ito, T. Ishikawa, K. Iwano, and N. Nishiya. 1995. Cloning and sequencing of cellulase cDNA from Aspergillus kawachii and its expression in Saccharomyces cerevisiae. Curr. Genet. 27: 435-439.
29. Saloheimo, A., B. Henrissat, A. M. Hoffren, O. Teleman, and M. Penttila. 1994. A novel small endoglucanase gene, egl5, from Trichoderma reesei isolated by expression in yeast. Mol. Microbiol. 13: 219-228.
30. Sheppard, P. O., F. J. Grant, P. J. Oort, C. A. Sprecher, D. C. Foster, F. S. Hagen, A. U. Upshall, G. L. McKnight, and P. J. OHara. 1994. The use of conserved cellulase family-specific sequences to clone cellulase homologue cDNAs from Fusarium oxysporum. Gene 150: 163-167.
31. Shuyan, L., D. Xinyuan, L. Xuemei, and G. Peiji. 2006. A novel thermophilic endoglucanase from a mesophilic fungus Fusarium oxysporum. Chinese Sci. Bull. 51: 191-197.
32. Sinclair, G. and F. Y. M. Choy. 2002. Synonymous codon usage bias and the expression of human glucocerebrosidase in the methylotrophic yeast Pichia pastoris. Protein Expr. Purif. 26: 96-105.
33. Teather, R. M. and P. J. Wood. 1982. Use of Congo redpolysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl. Environ. Microbiol. 8: 777-780.
34. Teng, D., Y. Fan, Y. L. Yang, Z. G. Tian, L. Luo, and J. H. Wang. 2007. Codon optimization of Bacillus licheniformis β- 1,3-1,4-glucanase gene and its expression in Pichia pastoris. Appl. Microbiol. Biotechnol. 74: 1074-1083.
35. Vervoort, E. B., A. Van Ravestein, N. N. van Peij, J. C. Heikoop, P. J. van Hasstert, G. F. Verheijden, and M. H. Linskens. 2000. Optimizing heterologous expression in Dictyostelium: Importance of 5' codon adaptation. Nucleic Acids Res. 28: 2069-2074.
36. Xiong, A. S., Q. H. Yao, and R. H. Peng. 2004. A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences. Nucleic Acids Res. 32: e98.