Monitoring bacterial faecal contamination in waters using multiplex real-time PCR assay for Bacteroides spp. and faecal enterococci

Water SA

Volumn 36, Issue 1, 2010, Pages 127-132

  Agudelo, R M ^a,b   Codony, F ^a   Adrados, B ^a   Fittipaldi, M ^a   Penuela G ^b   Morato J ^a  

^a UNIVERSITAT POLITÈCNICA DE CATALUNYA   (Spain)

^b UNIVERSIDAD DE ANTIOQUIA   (Colombia)

Author keywords

Bacteroides spp.; Faecal contamination; Faecal enterococci; Multiplex real time PCR; Water monitoring

Indexed keywords

ANALYSIS TIME; BACTERIAL INDICATORS; BACTEROIDES; CONVENTIONAL METHODS; DETECTION LEVELS; ENVIRONMENTAL ANALYSIS; FAECAL CONTAMINATION; INTERNAL INHIBITION; MULTIPLEX ASSAYS; REAL-TIME PCR; REAL-TIME PCR ASSAY; TRUE NEGATIVE; TRUE POSITIVE; WATER MONITORING; WATER SAMPLES;

BACTERIOLOGY; ESCHERICHIA COLI; MONITORING; MULTIPLEXING; POLLUTION DETECTION; WATER POLLUTION; WATER TREATMENT;

CONTAMINATION;

FECAL COLIFORM; POLLUTION MONITORING; POLYMERASE CHAIN REACTION; REAL TIME; SAMPLING; SANITATION; WATER POLLUTION;

BACTERIA (MICROORGANISMS); BACTEROIDES; BACTEROIDES SP.; ENTEROCOCCUS; ESCHERICHIA COLI;

EID: 77954230703     PISSN: 03784738     EISSN: 18167950     Source Type: Journal    
DOI: None     Document Type: Article

References (38)

1. ASHBOLT NJ, GRABOW WOK and SNOZZI M (2001) Indicators of microbial water quality. In: Fewtrell L and Bartram J (eds.) Water Quality: Guidelines, Standards and Health. Assessment of Risk and Risk Management for Water Related Infectious Disease. WHO Water Series. IWA Publishing, London. 285-315.
2. BAE S and WUERTZ S (2009) Discrimination of Viable and Dead Fecal Bacteroidales Bacteria by Quantitative PCR with Propidium Monoazide. Appl. Environ. Microbiol. 75 2940-2944.
3. BEJ AK, MAHBUBANI MH and ATLAS RM (1991) Detection of viable Legionella pneumophila in water by polymerase chain reaction and gene probe methods. Appl. Environ. Microbiol. 57 (2) 597-600.
4. BEN-DOV E, BRENNER A and KUSHMARO A (2007) Quantification of sulfate-reducing bacteria in industrial wastewater, by real-time polymerase chain reaction (PCR) using dsrA and apsA genes. Microb. Ecol. 54 (3) 439-451.
5. BRANLEY JM, ROY B, DWYER DE and SORREL TC (2008) Realtime PCR detection and quantitation of Chlamydophila psittaci in human and avian specimens from a veterinary clinic cluster. Eur. J. Clin. Microbiol. Infect. Dis. 27 269-273.
6. CAWTHORN DM and WITTHUHN RC (2008) Selective PCR detection of viable Enterobacter sakazakii cells utilizing propidium monoazide or ethidium bromide monoazide. J. Appl. Microbiol. 105 1178-1185.
7. COSTAFREDA M, BOSCH A and PINTO R (2006) Development, evaluation, and standardization of a real-time TaqMan reverse transcription-PCR assay, for quantification of Hepatitis A virus in clinical and shellfish samples. Appl. Environ. Microbiol. 72 (6) 3846-3855.
8. GILBRIDE KA, LEE DY and BEAUDETTE LA (2006) Molecular techniques in wastewater: understanding microbial communities, detecting pathogens, and real-time process control. J. Microbiol. Method 66 1-20.
9. HE JW and JIANG S (2005) Quantification of enterococci and human adenoviruses in environmental samples by real-time PCR. Appl. Environ. Microbiol. 71 (5) 2250-2255.
10. HUSSONG D, COLWELL RR, O'BRIEN MO, WEISS E, PEARSON AD, WEINER RM and BURGE WD (1987) Viable Legionella pneumophila not detectable by culture on agar media. Biotechnol. 5 947-950.
11. ISO (2000) Water Quality - Guidance on Validation of Micro biological Methods. ISO/TR 13843:2000. Geneva: International Organization for Standardization.
12. ICONTEC (2003) Calidad del agua Guía para la orientación acerca de la validación de métodos de análisis microbiológicos. GTC/ 84. Bogotá: Instituto Colombiano de Normas Técnicas y Certificación.
13. JOLY P, FALCONNET PA, ANDRÉ J, WEILL N, REYROLLE M, VANDENESCH F, MAURIN M and JARRAUD S (2006) Quantitative real-time Legionella PCR for environmental water samples: data interpretation. Appl. Environ. Microbiol. 72 (4) 2801-2808.
14. JONES TH, HOUDE A, POITRA E, WARD P and JOHNS MW (2009) Development and evaluation of o Multiplexed Real-time TaqMan RT-PCR assay with a sample process control for detection of F-specific RNA coliphage genogroups I and IV. Food Environ. Virol. DOI 10.1007/s12560-009-9008-7.
15. JOSEPHSON KL, GERBA CP and PEPPER IL (1993) Polymerase chain reaction detection of nonviable bacterial pathogens. Appl. Environ. Microbiol. 59 3513-3515.
16. KALINA O, LEBEDEVA I, BROWN J and SILVER J (1997) Nanoliter scale PCR with TaqMan detection. Nucleic Acids Res. 25 (10) 1999-2004.
17. LAYTON A, McKAY L, WILLIAMS D, GARRETT VR and SAYLER G (2006) Development of bacteroides 16S rRNA gene taqman-based real-time PCR assays for estimation of total, human and bovine faecal pollution in water. Appl. Environ. Microbiol. 72 (6) 4214-4224.
18. MANZÁN MA, ALJINOVIC EM, BIEDMA ME, SCIOCCO-CAP A, GHIRINGHELLI PD and ROMANOWISKI V (2008) Multiplex PCR and quality control of Epinotia aporema granulovirus production. Virus Genes 37 (2) 203-211.
19. NOCKER A, CHEUNG CY and CAMPER AK (2006) Comparison of propidium monoazide and ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. J. Microbiol. Methods 67 310-320.
20. NOGVA HK, DROMTORP SM, NISSEN H, and RUDI K (2003) Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5′-nuclease. PCR. Biotech. 810 812-813.
21. OLIVIER JD (2000) The public health significance of viable but non-culturable bacteria. In: Colwell RR and Grimes DJ (eds.) Nonculturable Microorganisms in the Environment. ASM Press, Washington DC. 277-300.
22. PUSCH D, OH DY, WOLF S, DUMKE R, SCHRÖTER-BOBSIN U, HÖNE M, RÖSKE I and SCHREIER E (2005) Detection of enteric viruses and bacterial indicators in German environmental waters. Arch. Virol. 150 929-947.
23. RASMUSSEN R (2001) Quantification on the light cycler. In: Meuer S, Witwe C and Nakagawara K (eds.) Rapid Cycler Real-Time PCR, Methods and Applications. Springer Press, New York. 21-34.
24. RAWSTHORNE H, DOCK CN and JAYKUS LA (2009) PCR-Based method using propidium monoazide to distinguish viable from non viable Bacillus subtilis spores. Appl. Environ. Microbiol. 75 2936-2939.
25. REISCHER GF, HAIDER JM, SOMME R, STADLER H, KEIBLINGER KM, HORNEK R, ZEROBIN W, MACH RL and FARNLEITNER AH (2008) Quantitative microbial faecal source tracking with sampling guided by hydrological catchment dynamics. Environ. Microbiol. 10 2598-2608.
26. ROSE JB (2006) Identification and characterization of biological risks for water. In: Management of Intentional and Accidental Water Pollution. Nato Security through Science Series. Springer, Netherlands. 85-101.
27. ROSE L, HERRA CM and CROWLEY B (2008) Evaluation of realtime polymerase chain reaction assays for the detection of herpes simplex virus in swab specimens. Eur. J. Clin. Microbiol. Infect. Dis. 27 827-861.
28. RUDI K, MOEN B, DRØMTORP SM and HOLCK L (2005) Use of Ethidium Monoazide and PCR in Combination for Quantification of Viable and Dead Cells in Complex Samples. Appl. Environ. Microbiol. 71 1018-1024.
29. SARTORY DP (2005) Validation, verification and comparison: adopting new methods in water microbiology. Water SA 31 (3) 393-396.
30. SAVICHTCHEVA O and OKABE S (2006) Alternative indicators of faecal pollution: Relations with pathogens and conventional indicators, current methodologies for direct pathogen monitoring and future application perspectives. Water Res. 40 2463-2476.
31. SHIH HY and LIN YE (2006) Letter to the Editor. Caution on interpretation of Legionella results obtained using real-time PCR for environmental water samples. Appl. Environ. Microbiol. 72 (10) 6859.
32. SINGH S and SINGH S (2008) Real-time polymerase chain reaction. A revolution in diagnosis. Resonance. 13 (4) 369-377.
33. SOEJIMA T, IIDA K, QIN T, TANIAI H, SEKI M, TAKADE A and YOSHIDA S (2007) Photo activated ethidium monoazide directly cleaves bacterial DNA and is applied to PCR for discrimination of live and dead bacteria. Microbiol. Immunol. 51 763-775.
34. STANDARD METHODS (2005) Standard Methods for the Examination of Water and Wastewater (Eaton AD, Clesceri LS, Rice EW and Greenberg AE) (21st edn.). United Book Press, USA. 9-111 - 9-136.
35. STRICKER AR, WILHARTITZ I, FARNLEITNER AH and MACH RL (2008) Development of a Scorpion probe-based real-time PCR for the sensitive quantification of Bacteroides sp. ribosomal DNA from human and cattle origin and evaluation in spring water matrices. Microbiol. Res. 163 (2) 140-147.
36. SZEWZYK U, SZEWZYK R, MANZ W and SCHLEIFER KH (2000) Microbiological safety of drinking water. Annu. Rev. Microbiol. 54 81-127.
37. TAJIMA A, SAKURAI K and MINAMIYAMA M (2007) Behavior of pathogenic microbes in a treated wastewater reuse system and examination of new standards for the reuse of treated wastewater. Environ. Monit. Assess. 129 43-51.
38. VESPER S, McKINSTRY C, HARTMANN C, NEACE M, YODER S and VESPER A (2008) Quantifying fungal viability in air and water samples using quantitative PCR after treatment with propidium monoazide (PMA). J. Microbiol. Methods 72 180-184.