Caution on interpretation of Legionella results obtained using real-time PCR for environmental water samples [1]

Applied and Environmental Microbiology

Volumn 72, Issue 10, 2006, Pages 6859-

  Shih, Hsiu Yun ^a   Lin, Yusen Eason ^a  

^a NATIONAL KAOHSIUNG NORMAL UNIVERSITY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL RNA; COMPLEMENTARY DNA; DRINKING WATER; MESSENGER RNA; NUCLEIC ACID; PROTEIN MIP; UNCLASSIFIED DRUG;

BACTERIUM CULTURE; CELL VIABILITY; COOLING TOWER; DISINFECTION; FALSE POSITIVE RESULT; INFECTION RISK; LEGIONELLA; LETTER; METHODOLOGY; NONHUMAN; QUANTITATIVE ASSAY; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION; RNA ANALYSIS; WATER ANALYSIS; WATER SAMPLING;

DNA, BACTERIAL; ENVIRONMENT; FALSE POSITIVE REACTIONS; FRESH WATER; LEGIONELLA; POLYMERASE CHAIN REACTION; WATER MICROBIOLOGY;

LEGIONELLA;

EID: 33750083899     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.00968-06     Document Type: Letter

References (6)

1. Eej, A. K., M. H. Mahbubani, and R. M. Atlas. 1991. Detection of viable Legionella pneumophila in water by polymerase chain reaction and gene probe methods. Appl. Environ. Microbiol. 57:597-600.
2. Joly, P., P.-A. Falconnet, J. André, N. Weill, M. Reyrolle, F. Vandenesch, M. Maurin, J. Etienne, and S. Jarraud. 2006. Quantitative real-time Legionella PCR for environmental water samples: data interpretation. Appl. Environ. Microbiol. 72:2801-2808.
3. Wellinghausen, N., C. Frost, and R. Marre. 2001. Detection of legionellae in hospital water samples by quantitative real-time LightCycler PCR. Appl. Environ. Microbiol. 67:3985-3993.
4. Wilson, I. G. 1997. Inhibition and facilitation of nucleic acid amplification. Appl. Environ. Microbiol. 63:3741-3751.
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6. Yamamoto, H., Y. Hashimoto, and T. Ezaki. 1996. Study of nonculturable Legionella pneumophila cells during multiple-nutrient starvation. FEMS Microbiol. Ecol. 20:149-154.