Biochemical characterisation of Murray Valley encephalitis virus proteinase

FEBS Letters

Volumn 584, Issue 14, 2010, Pages 3149-3152

  Joy, Joma ^a   Mee, Ng Fui ^a   Kuan, Wee Liang ^a   Perlyn, Kwek Zekui ^a   Wen, Then Siew ^a   Hill, Jeffrey ^a  

^a Agency for Science   (Singapore)

Author keywords

Dengue; Flavivirus; Inhibitor; Murray Valley encephalitis; Protease; West Nile virus

Indexed keywords

HYBRID PROTEIN; NONSTRUCTURAL PROTEIN 2; NONSTRUCTURAL PROTEIN 2B; NONSTRUCTURAL PROTEIN 3; POLYPROTEIN; PROTEINASE; SERINE PROTEINASE; UNCLASSIFIED DRUG; VIRUS ENZYME; VIRUS PROTEIN;

ARTICLE; BIOCHEMISTRY; CONTROLLED STUDY; ENZYME ANALYSIS; MOLECULAR CLONING; MURRAY VALLEY ENCEPHALITIS VIRUS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PROCESSING; VIRUS GENOME; FLAVIVIRUS; GENETICS; JAPANESE ENCEPHALITIS VIRUS; METABOLISM;

FLAVIVIRUS; MURRAY VALLEY ENCEPHALITIS VIRUS; RNA VIRUSES; WEST NILE VIRUS;

ENCEPHALITIS VIRUS, MURRAY VALLEY; ENDOPEPTIDASES; FLAVIVIRUS; POLYPROTEINS; PROTEIN PROCESSING, POST-TRANSLATIONAL; SERINE PROTEASES; VIRAL PROTEINS;

EID: 77954177299     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2010.05.062     Document Type: Article

References (24)

1. Mukhopadhyay S., Kuhn R.J., Rossmann M.G. A structural perspective of the flavivirus life cycle. Nat. Rev. Microbiol. 2005, 3:13-22.
2. Kuno G., Chang G.J., Tsuchiya K.R., Karabatsos N., Cropp C.B. Phylogeny of the genus Flavivirus. J. Virol. 1998, 72:73-83.
3. Mackenzie J.S., Broom A.K. Australian X disease, Murray Valley encephalitis and the French connection. Vet. Microbiol. 1995, 46:79-90.
4. Chambers T.J., Hahn C.S., Galler R., Rice C.M. Flavivirus genome organization, expression, and replication. Annu. Rev. Microbiol. 1990, 44:649-688.
5. Assenberg R., Mastrangelo E., Walter T.S., Verma A., Milani M., Owens R.J., Stuart D.I., Grimes J.M., Mancini E.J. Crystal structure of a novel conformational state of the Flavivirus NS3 protein: implications for polyprotein processing and viral replication. J. Virol. 2009, 83:12895-12906.
6. Chappell K.J., Stoermer M.J., Fairlie D.P., Young P.R. Insights to substrate binding and processing by West Nile Virus NS3 protease through combined modeling, protease mutagenesis, and kinetic studies. J. Biol. Chem. 2006, 281:38448-38458.
7. Leung D., Schroder K., White H., Fang N.X., Stoermer M.J., Abbenante G., Martin J.L., Young P.R., Fairlie D.P. Activity of recombinant dengue 2 virus NS3 protease in the presence of a truncated NS2B cofactor, small peptide substrates, and inhibitors. J. Biol. Chem. 2001, 276:45762-45771.
8. Nall T.A., Chappell K.J., Stoermer M.J., Fang N.X., Tyndall J.D., Young P.R., Fairlie D.P. Enzymatic characterization and homology model of a catalytically active recombinant West Nile virus NS3 protease. J. Biol. Chem. 2004, 279:48535-48542.
9. Bessaud M., Pastorino B.A., Peyrefitte C.N., Rolland D., Grandadam M., Tolou H.J. Functional characterization of the NS2B/NS3 protease complex from seven viruses belonging to different groups inside the genus Flavivirus. Virus Res. 2006, 120:79-90.
10. Erbel P., Schiering N., D'Arcy A., Renatus M., Kroemer M., Lim S.P., Yin Z., Keller T.H., Vasudevan S.G., Hommel U. Structural basis for the activation of flaviviral NS3 proteases from dengue and West Nile virus. Nat. Struct. Mol. Biol. 2006, 13:372-373.
11. Chappell K.J., Stoermer M.J., Fairlie D.P., Young P.R. Generation and characterization of proteolytically active and highly stable truncated and full-length recombinant West Nile virus NS3. Protein Expression Purif. 2007, 53:87-96.
12. Gao W., Rzewski A., Sun H., Robbins P.D., Gambotto A. UpGene: application of a web-based DNA codon optimization algorithm. Biotechnol. Prog. 2004, 20:443-448.
13. Hill J., Tyas L., Phylip L.H., Kay J., Dunn B.M., Berry C. High level expression and characterisation of Plasmepsin II, an aspartic proteinase from Plasmodium falciparum. FEBS Lett. 1994, 352:155-158.
14. Tellier E., Negre-Salvayre A., Bocquet B., Itohara S., Hannun Y.A., Salvayre R., Auge N. Role for furin in tumor necrosis factor alpha-induced activation of the matrix metalloproteinase/sphingolipid mitogenic pathway. Mol. Cell. Biol. 2007, 27:2997-3007.
15. Bessaud M., Grard G., Peyrefitte C.N., Pastorino B., Rolland D., Charrel R.N., de L.X., Tolou H.J. Identification and enzymatic characterization of NS2B-NS3 protease of Alkhurma virus, a class-4 flavivirus. Virus Res. 2005, 107:57-62.
16. Steuer C., Heinonen K.H., Kattner L., Klein C.D. Optimization of assay conditions for dengue virus protease: effect of various polyols and nonionic detergents. J. Biomol. Screen. 2009, 14:1102-1108.
17. Li J., Lim S.P., Beer D., Patel V., Wen D., Tumanut C., Tully D.C., Williams J.A., Jiricek J., Priestle J.P., Harris J.L., Vasudevan S.G. Functional profiling of recombinant NS3 proteases from all four serotypes of dengue virus using tetrapeptide and octapeptide substrate libraries. J. Biol. Chem. 2005, 280:28766-28774.
18. Charbaut E., Redeker V., Rossier J., Sobel A. N-terminal acetylation of ectopic recombinant proteins in Escherichia coli. FEBS Lett. 2002, 529:341-345.
19. Peyrefitte C.N., Pastorino B.A., Grandadam M., Rolland D., Tolou H.J., Bessaud M. Enzymatic characterization of a trypsin-like serine protease encoded by the genome of cell fusing agent virus. Virus Genes 2007, 34:185-191.
20. Kacprzak M.M., Peinado J.R., Than M.E., Appel J., Henrich S., Lipkind G., Houghten R.A., Bode W., Lindberg I. Inhibition of furin by polyarginine-containing peptides: nanomolar inhibition by nona-D-arginine. J. Biol. Chem. 2004, 279:36788-36794.
21. Shiryaev S.A., Ratnikov B.I., Chekanov A.V., Sikora S., Rozanov D.V., Godzik A., Wang J., Smith J.W., Huang Z., Lindberg I., Samuel M.A., Diamond M.S., Strongin A.Y. Cleavage targets and the D-arginine-based inhibitors of the West Nile virus NS3 processing proteinase. Biochem. J. 2006, 393:503-511.
22. Robin G., Chappell K., Stoermer M.J., Hu S.H., Young P.R., Fairlie D.P., Martin J.L. Structure of West Nile virus NS3 protease: ligand stabilization of the catalytic conformation. J. Mol. Biol. 2009, 385:1568-1577.
23. Johnston P.A., Phillips J., Shun T.Y., Shinde S., Lazo J.S., Huryn D.M., Myers M.C., Ratnikov B.I., Smith J.W., Su Y., Dahl R., Cosford N.D., Shiryaev S.A., Strongin A.Y. HTS identifies novel and specific uncompetitive inhibitors of the two-component NS2B-NS3 proteinase of West Nile virus. Assay Drug Dev. Technol. 2007, 5:737-750.
24. Lobigs M., Larena M., Alsharifi M., Lee E., Pavy M. Live chimeric and inactivated Japanese encephalitis virus vaccines differ in their cross-protective values against Murray Valley encephalitis virus. J. Virol. 2009, 83:2436-2445.