Chemopreventive potential of β-Sitosterol in experimental colon cancer model - an In vitro and In vivo study

BMC Complementary and Alternative Medicine

Volumn 10, Issue , 2010, Pages

  Baskar, Albert A ^a,b   Ignacimuthu, Savarimuthu ^a   Paulraj, Gabriel M ^a   Al Numair, Khalid S ^b  

^a LOYOLA COLLEGE   (India)

^b KING SAUD UNIVERSITY   (Saudi Arabia)

Author keywords

[No Author keywords available]

Indexed keywords

1,2 DIMETHYLHYDRAZINE; BETA CATENIN; CYCLINE; REACTIVE OXYGEN METABOLITE; SITOSTEROL; ANTINEOPLASTIC AGENT; ANTIOXIDANT; PLANT EXTRACT; SITOSTEROL DERIVATIVE;

ANIMAL CELL; ANIMAL CELL CULTURE; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIGEN EXPRESSION; ANTINEOPLASTIC ACTIVITY; APOPTOSIS; ARTICLE; ASCLEPIAS; ASCLEPIAS CURASSAVICA; BIOASSAY; CANCER CELL CULTURE; CELL PROLIFERATION; COLON ADENOCARCINOMA; COLON CARCINOGENESIS; CONCENTRATION RESPONSE; CONTROLLED STUDY; CRYPT CELL; CYTOTOXICITY; DRUG IDENTIFICATION; DRUG ISOLATION; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IN VITRO STUDY; IN VIVO STUDY; KIDNEY CELL; MALE; NONHUMAN; PROTEIN EXPRESSION; RAT; SUPPLEMENTATION; ADENOCARCINOMA; ANIMAL; CELL LINE; CHEMISTRY; COLON; COLON TUMOR; DISEASE MODEL; DOSE RESPONSE; DRUG EFFECT; HAPLORHINI; ISOLATION AND PURIFICATION; KIDNEY; METABOLISM; PATHOLOGY; PHYTOTHERAPY; TUMOR CELL LINE; WISTAR RAT;

ADENOCARCINOMA; ANIMALS; ANTINEOPLASTIC AGENTS, PHYTOGENIC; ANTIOXIDANTS; APOPTOSIS; ASCLEPIAS; BETA CATENIN; CELL LINE; CELL LINE, TUMOR; COLON; COLONIC NEOPLASMS; DISEASE MODELS, ANIMAL; DOSE-RESPONSE RELATIONSHIP, DRUG; HAPLORHINI; HUMANS; KIDNEY; MALE; PHYTOTHERAPY; PLANT EXTRACTS; PROLIFERATING CELL NUCLEAR ANTIGEN; RATS; RATS, WISTAR; SITOSTEROLS;

EID: 77954146578     PISSN: None     EISSN: 14726882     Source Type: Journal    
DOI: 10.1186/1472-6882-10-24     Document Type: Article

References (38)

1. Bingham S, Riboli E. Diet and cancer--the European prospective investigation into cancer and nutrition. Nat Rev Cancer 2004, 4:206-215. 10.1038/nrc1298, 14993902.
2. Johnson IT, Williamson G, Musk SR. Anticarcinogenic factors in plant foods: a new class of nutrients. Nutr Rev 1994, 7:175-204.
3. Awad AB, Downie AC, Fink CS. Inhibition of growth and stimulation of apoptosis by β-sitosterol treatment of MDA-MB-231 human breast cancer cells in culture. Int J Mol Med 2000, 5:541-545.
4. Behrens J. The role of the Wnt signalling pathway in colorectal tumorigenesis. J Biochem Soc Trans 2005, 33:672-675.
5. Takahashi M, Fukuda K, Sugimura T, Wakabayashi K. β-Catenin is frequently mutated and demonstrates altered cellular location in azoxymethane-induced rat colon tumors. Cancer Res 1998, 56:42-46.
6. Sengottuvelan M, Senthilkumar R, Nalini N. Modulatory influence of dietary resveratrol during different phases of 1, 2-dimethylhydrazine induced mucosal lipid-peroxidation, antioxidant status and aberrant crypt foci development in rat colon carcinogenesis. Biochim Biophys Acta 2006, 1760:1175-1183.
7. Kupchan SM, Knox JR, Kelsey JE. Calotopin, a cytotoxic principle isolated from Asclepias curassavica L. Science 1964, 146:1685-1686. 10.1126/science.146.3652.1685, 14224519.
8. Awad AB, Chinnam M, Fink CS, Bradford PG. β-sitosterol activates Fas signaling in human breast cancer cells. Phytomedicine 2007, 14:747-754. 10.1016/j.phymed.2007.01.003, 17350814.
9. Moon DO, Kyeong-Jun L, Yung HC, Gi-Young K. β-Sitosterol-induced-apoptosis is mediated by the activation of ERK and the downregulation of Akt in MCA-102 murine fibrosarcoma cells. Int Immunopharmacol 2007, 7:1044-1053. 10.1016/j.intimp.2007.03.010, 17570321.
10. Jayaprakash GK, Mandadi KK, Poulose SM, Jadegoud Y, Nagana GGA, Patil BS. Inhibition of colon cancer cell growth and antioxidant activity of bioactive compounds from Poncirus trifoliata (L.) Raf. Bioorg Med Chem 2007, 14:4923-4932.
11. Vivancos M, Moreno JJ. Sitosterol modulates antioxidant enzyme response in RAW 264.7 macrophages. Free Radic Biol Med 2005, 39:91-97. 10.1016/j.freeradbiomed.2005.02.025, 15925281.
12. Awad AB, Andrew TB, Carol SF. Effect of resveratrol and β-sitosterol in combination on reactive oxygen species and prostaglandin release by PC-3 cells. Prostaglandins Leukot Essent Fatty Acids 2005, 72:219-226. 10.1016/j.plefa.2004.11.005, 15664307.
13. Moreno JJ. Effect of olive oil minor components on oxidative stress and arachidonic acid mobilization and metabolism by macrophages RAW 264.7. Free Radic Biol Med 2003, 35:1073-1081. 10.1016/S0891-5849(03)00465-9, 14572610.
14. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958, 181:1199-1200.
15. Garrat DC. The Quantitative Analysis of Drugs. 1964, 3:456-458. Chapman and Hall, Japan.
16. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983, 65:55-63. 10.1016/0022-1759(83)90303-4, 6606682.
17. Vermes I, Haanen C, Steffens-Nakken H, Reutelingsperger C. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J. Immunol Methods 1995, 184:39-51.
18. Bhushan S, Singh J, Rao MJ, Saxena AK, Qazi GN. A novel lignan composition from Cedrus deodara induces apoptosis and early nitric oxide generation in human leukemia Molt -4 and HL-60 cells. Nitric oxide 2006, 14:72-88.
19. Bird RP. Observation and quantification of aberrant crypts in the murine colon treated with a colon carcinogen: preliminary findings. Cancer Lett 1987, 37:147-151. 10.1016/0304-3835(87)90157-1, 3677050.
20. Emel Z, Pinar A, Emine K, Koray GM, Umit Z. Alterations in lipid peroxidation and antioxidant status in different types intracranial tumors within their relative peritumoral tissues. Clin Neurol Neurosurg 2009, 111:345-351. 10.1016/j.clineuro.2008.11.008, 19117666.
21. Sun SY, Hail N, Lotan R. Apoptosis as a novel target for cancer chemoprevention. J Natl Cancer Inst 2004, 96:662-672.
22. Finkel T, Holbrook NJ. Oxidants, oxidative stress and the biology of ageing. Nature 2000, 408:239-247. 10.1038/35041687, 11089981.
23. Johnson IT, Williamson G, Musk SR. Anticarcinogenic factors in plant foods: a new class of nutrients. Nutr Rev 1994, 7:175-204.
24. Muller I, Jenner A, Bruchelt G, Niethanmmer D, Halliwell B. Effect of concentration on the cytotoxic mechanism of doxorubicin-apoptosis and oxidative DNA damage. Biochem Biophys Res Commun 1997, 230:254-257. 10.1006/bbrc.1996.5898, 9016760.
25. Young SL, Jae HY, Man JB, Wang KY, Shen Y, Charlie CLX, Chun GL. Anti-oxidant and Anti-hypercholesterolemic Activities of Wasabia japonica. Evid. Based Complement. Altern. Med., Advance Access published on June 12, 2008 doi: doi:10.1093/ecam/nen038.
26. Nguyen AT, Jeanine F, Hugues M, Magda C, Michel L, Pierre D. A sugar ester and an iridoid glycoside from Scrophularia ningpoensis. Phytochemistry 2005, 66:1186-1191. 10.1016/j.phytochem.2005.03.023, 15924924.
27. Perry A, Jenkins RB, O'Fallon JR, Schaefer PL, Kimmel DW, Mahoney MR, Scheithauer BW, Smith SM, Hill EM, Sebo TJ, Levitt R, Krook J, Tschetter LK, Morton RF, Buckner JC. Clinicopathologic study of 85 similarly treated patients with anaplastic astrocytic tumors: An analysis of DNA content (ploidy), cellular proliferation, and p53 expression. Cancer 1999, 86:672-683. 10.1002/(SICI)1097-0142(19990815)86:4<672::AID-CNCR17>3.0.CO;2-G, 10440696.
28. Ziliotto L, Barbisan L, Rodrigues M. Lack of chemoprevention of dietary Agaricus blazei against rat colonic aberrant crypt foci. Hum Exp Toxicol 2008, 6:505-511.
29. Inamine M, Suzui M, Morioka T, Kinjo T, Kaneshiro T, Sugishita T, Okada T, Yoshimi N. Inhibitory effect of dietary monoglucosylceramide1-O-beta-glucosyl-N-2'-hydroxyarachidoyl-4,8-sphinga dienine on two different categories of colon preneoplastic lesions induced by 1,2-dimethylhydrazine in F344 rats. Cancer Sci 2005, 12:876-881.
30. Funato Y, Tatsuo M, Makoto A, Hiroaki M. The thioredoxin-related redox-regulating protein nucleoredoxin inhibits Wnt-β-catenin signalling through Dishevelled. Nat cell biol 2006, 8:5.
31. Porter AC, Vaillancourt RR. Tyrosine kinase receptor-activated signal transduction pathway which lead to oncogenesis. Oncogene 1998, 17:1343-1352. 10.1038/sj.onc.1202171, 9779982.
32. Damalas A, Ben-Ze'ev A, Simcha I, Shtutman M, Leal JF, Zhurinsky J, Geiger B, Oren M. Excess beta-catenin promotes accumulation of transcriptionally active p53. EMBO J 1999, 18:3054-3063. 10.1093/emboj/18.11.3054, 1171387, 10357817.
33. Awad AB, Fink CS. Phytosterols as anticancer dietary components: evidence and mechanism of action. J Nutr 2000, 130:2127-2130.
34. Bravo R, Frank R, Blundell PA, Macdonald-Bravo H. Cyclin/PCNA is the auxiliary protein of DNA polymerase-delta. Nature 1987, 326:515-517. 10.1038/326515a0, 2882423.
35. Toyokuni S, Okamoto K, Yodoi J, Hiai H. Hypothesis: persistent oxidative stress in cancer. FEBS Lett 1995, 358:1-3. 10.1016/0014-5793(94)01368-B, 7821417.
36. Hara A, Sakata K, Yamada Y, Kuno T, Kitaori N, Oyama T, Hirose Y, Murakami A, Tanaka T, Mori H. Suppression of β-catenin mutation by dietary exposure of auraptene, a citrus antioxidant, N,N-diethylnitrosamine - induced hepatocellular carcinoma in rats. Oncol Rep 2005, 14:345-351.
37. McLellan EA, Bird RP. Aberrant crypts: potential preneoplastic lesions in the murine colon. Cancer Res 1988, 21:6187-6192.
38. Mori H, Tanaka T, Sugie S, Yoshimi N, Kawamori T, Hirose Y, Ohnishi M. Chemoprevention by naturally occurring and synthetic agents in oral, liver, and large bowel carcinogenesis. J Cell Bioc 1997, 27:35-41.