Changes in the rumen bacterial community in response to sunflower oil and fish oil supplements in the diet of dairy sheep

Journal of Dairy Science

Volumn 93, Issue 7, 2010, Pages 3275-3286

  Belenguer, A ^a   Toral, P G ^a   Frutos, P ^a   Hervas G ^a  

^a UNIVERSITY OF LEÓN   (Spain)

Author keywords

Ewe; Lipid supplementation; Molecular technique; Rumen microbiota

Indexed keywords

FISH OIL; RNA 16S; SUNFLOWER OIL; VEGETABLE OIL;

ANIMAL; ANIMAL DISEASE; ARTICLE; BACTERIAL PHENOMENA AND FUNCTIONS; BACTERIUM; BIODIVERSITY; DAIRYING; DIET; DIET SUPPLEMENTATION; GENETICS; MICROBIOLOGY; RANDOMIZATION; RUMINANT STOMACH; SHEEP;

ANIMALS; BACTERIA; BACTERIAL PHYSIOLOGICAL PHENOMENA; BIODIVERSITY; DAIRYING; DIET; DIETARY SUPPLEMENTS; FISH OILS; PLANT OILS; RANDOM ALLOCATION; RNA, RIBOSOMAL, 16S; RUMEN; SHEEP;

ACIDAMINOCOCCACEAE; ANIMALIA; BACTERIA (MICROORGANISMS); BUTYRIVIBRIO; HELIANTHUS; LACHNOSPIRACEAE; OVIS ARIES; QUINELLA;

EID: 77953890446     PISSN: 00220302     EISSN: 15253198     Source Type: Journal    
DOI: 10.3168/jds.2010-3101     Document Type: Article

References (46)

1. Amann R.I., Binder B.J., Olson R.J., Chisholm S.W., Devereux R., Stahl D.A. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 1990, 56:1919-1925.
2. Belenguer A., Duncan S.H., Holtrop G., Anderson S.E., Lobley G.E., Flint H.J. Impact of pH on lactate formation and utilization by human fecal microbial communities. Appl. Environ. Microbiol. 2007, 73:6526-6533.
3. Boeckaert C., Fievez V., Van Hecke D., Verstraete W., Boon N. Changes in rumen biohydrogenation intermediates and ciliate protozoa diversity after algae supplementation to dairy cattle. Eur. J. Lipid Sci. Technol. 2007, 109:767-777.
4. Boeckaert C., Morgavi D.P., Jouany J.P., Maignien L., Boon N., Fievez V. Role of the protozoan Isotricha prostoma, liquid-, and solid-associated bacteria in rumen biohydrogenation of linoleic acid. Animal 2009, 3:961-971.
5. Boeckaert C., Vlaeminck B., Fievez V., Maignien L., Dijkstra J., Boon N. Accumulation of trans C18:1 fatty acids in the rumen after dietary algal supplementation is associated with changes in the Butyrivibrio community. Appl. Environ. Microbiol. 2008, 74:6923-6930.
6. Castillo M., Martin-Orue S.M., Nofrarias M., Manzanilla E.G., Gasa J. Changes in caecal microbiota and mucosal morphology of weaned pigs. Vet. Microbiol. 2007, 124:239-247.
7. Cole J.R., Wang Q., Cardenas E., Fish J., Chai B., Farris R.J., Kulam-Syed-Mohideen A.S., McGarrell D.M., Marsh T., Garrity G.M., Tiedje J.M. The Ribosomal Database Project: Improved alignments and new tools for rRNA analysis. Nucleic Acids Res. 2009, 37:D141-D145.
8. Collins M.D., Lawson P.A., Willems A., Cordoba J.J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J.A.E. The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combination. Int. J. Syst. Bacteriol. 1994, 44:812-826.
9. Deng W.D., Wanapat M., Ma S.C., Chen J., Xi D.M., He T.B., Yang Z.F., Mao H.M. Phylogenetic analysis of 16S rDNA sequences manifest rumen bacterial diversity in gayals (Bos frontalis) fed fresh bamboo leaves and twigs (Sinarumdinaria). Asian-australas. J. Anim. Sci. 2007, 20:1057-1066.
10. Devillard E., McIntosh F.M., Duncan S.H., Wallace R.J. Metabolism of linoleic acid by human gut bacteria: Different routes for biosynthesis of conjugated linoleic acid. J. Bacteriol. 2007, 189:2566-2570.
11. Egan A.R. Experimental designs for rumen microbiology. Methods in Gut Microbial Ecology for Ruminants 2005, 3-22. Springer, New York, NY. H.P.S. Makkar, C.S. McSweeney (Eds.).
12. Franks A.H., Harmsen H.J., Raangs G.C., Jansen G.J., Schut F., Welling G.W. Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes. Appl. Environ. Microbiol. 1998, 64:3336-3345.
13. Fuentes M.C., Calsamiglia S., Cardozo P.W., Vlaeminck B. Effect of pH and level of concentrate in the diet on the production of biohydrogenation intermediates in a dual-flow continuous culture. J. Dairy Sci. 2009, 92:4456-4466.
14. Harfoot C.G., Hazlewood G.P. Lipid metabolism in the rumen. The Rumen Microbial Ecosystem 1997, 382-426. Chapman & Hall, London, UK. P.N. Hobson, C.S. Stewart (Eds.).
15. Hill T.C.J., Walsh K.A., Harris J.A., Moffett B.F. Using ecological diversity measures with bacterial communities. FEMS Microbiol. Ecol. 2003, 43:1-11.
16. Hongoh Y., Yuzawa H., Ohkuma M., Kudo T. Evaluation of primers and PCR conditions for the analysis of 16S rRNA genes from a natural environment. FEMS Microbiol. Lett. 2003, 221:299-304.
17. Jenkins T.C., Wallace R.J., Moate P.J., Mosley E.E. Board-invited review: Recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem. J. Anim. Sci. 2008, 86:397-412.
18. Kemp P., White R.W., Lander D.J. The hydrogenation of unsaturated fatty acids by five bacterial isolates from the sheep rumen, including a new species. J. Gen. Microbiol. 1975, 90:100-114.
19. Kim E.J., Huws S.A., Lee M.R., Wood J.D., Muetzel S.M., Wallace R.J., Scollan N.D. Fish oil increases the duodenal flow of long chain polyunsaturated fatty acids and trans-11 18:1 and decreases 18:0 in steers via changes in the rumen bacterial community. J. Nutr. 2008, 138:889-896.
20. Kitts C.L. Terminal restriction fragment patterns: A tool for comparing microbial communities and assessing community dynamics. Curr. Issues Intest. Microbiol. 2001, 2:17-25.
21. Krumholz L.R., Bryant M.P., Brulla W.J., Vicini J.L., Clark J.H., Stahl D.A. Proposal of Quinella ovalis gen. nov., sp. nov., based on phylogenetic analysis. Int. J. Syst. Bacteriol. 1993, 43:293-296.
22. Lee M.R.F., Shingfield K.J., Tweed J.K.S., Toivonen V., Huws S.A., Scollan N.D. Effect of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids in steers fed grass or red clover silages. Animal 2008, 2:1859-1869.
23. Li M., Penner G.B., Hernandez-Sanabria E., Oba M., Guan L.L. Effects of sampling location and time, and host animal on assesment of bacterial diversity and fermentation parameters in the bovine rumen. J. Appl. Microbiol. 2009, 10.1111/j.1365-2672.2009.04376.x.
24. Maeda H., Fujimoto C., Haruki Y., Maeda T., Kokeguchi S., Petelin M., Arai H., Tanimoto I., Nishimura F., Takashiba S. Quantitative real-time PCR using TaqMan and SYBR Green for Actinobacillus actinomicetescomitans, Porphyromonas gingivalis, Prevotella intermedia, tetQ gene and total bacteria. FEMS Immunol. Med. Microbiol. 2003, 39:81-86.
25. Maia M.R., Chaudhary L.C., Figueres L., Wallace R.J. Metabolism of polyunsaturated fatty acids and their toxicity to the microflora of the rumen. Antonie van Leeuwenhoek 2007, 91:303-314.
26. Moon C.D., Pacheco D.M., Kelly W.J., Leahy S.C., Li D., Kopecny J., Attwood G.T. Reclassification of Clostridium proteoclasticum as Butyrivibrio proteoclasticus comb. nov., a butyrate-producing ruminal bacterium. Int. J. Syst. Evol. Microbiol. 2008, 58:2041-2045.
27. Mould F.L., Kliem K.E., Morgan R., Mauricio R.M. In vitro microbial inoculum: A review of its function and properties. Anim. Feed Sci. Technol. 2005, 123:31-50.
28. Orpin C.G. The culture in vitro of the rumen bacterium Quin's Oval. J. Gen. Microbiol. 1972, 73:523-530.
29. Paillard D., McKain N., Chaudhary L.C., Walker N.D., Pizette F., Koppova I., McEwan N.R., Kopecny J., Vercoe P.E., Louis P., Wallace R.J. Relation between phylogenetic position, lipid metabolism and butyrate production by different Butyrivibrio-like bacteria from the rumen. Antonie van Leeuwenhoek 2007, 91:417-422.
30. Paillard D., McKain N., Rincon M.T., Shingfield K.J., Givens D.I., Wallace R.J. Quantification of ruminal Clostridium proteoclasticum by real-time PCR using a molecular beacon approach. J. Appl. Microbiol. 2007, 103:1251-1261.
31. Palmquist D.L., Lock A.L., Shingfield K.J., Bauman D.E. Biosynthesis of conjugated linoleic acid in ruminants and humans. Adv. Food Nutr. Res. 2005, 50:179-217.
32. Pfaffl M.W. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001, 29:2002-2007.
33. Shingfield K.J., Ahvenjarvi S., Toivonen V., Arola A., Nurmela K.V.V., Huhtanen P., Griinari J.M. Effect of dietary fish oil on biohydrogenation of fatty acids and milk fatty acid content in cows. Anim. Sci. 2003, 77:165-179.
34. Shingfield K.J., Bernard L., Leroux C., Chilliard Y. Trans fatty acids and mammary lipogenesis in ruminants. XIth International Symposium on Ruminant Physiology. Ruminant Physiology. Digestion, Metabolism, and Effects of Nutrition on Reproduction and Welfare 2009, 56. Y. Chilliard, F. Glasser, Y. Faulconnier, F. Bocquier, I. Veissier, M. Doreau (Eds.).
35. Shingfield K.J., Reynolds C.K., Hervás G., Griinari J.M., Grandison A.S., Beever D.E. Examination of the persistency of milk fatty acid composition responses to fish oil and sunflower oil in the diet of dairy cows. J. Dairy Sci. 2006, 89:714-732.
36. Tajima K., Aminov R.I., Nagamine T., Ogama K., Nakamura M., Matsui H., Benno Y. Rumen bacterial diversity as determined by sequence analysis of 16S rDNA libraries. FEMS Microbiol. Ecol. 1999, 29:159-169.
37. Tolosa, M. X. 2006. Molecular ecology of rumen bacterial populations in steers fed molasses diets. MS Thesis. School of Animal Studies, The University of Queensland, Brisbane, Australia.
38. Toral P.G., Belenguer A., Frutos P., Hervás G. Effect of the supplementation of a high-concentrate diet with sunflower and fish oils on ruminal fermentation in sheep. Small Rumin. Res. 2009, 81:119-125.
39. Toral P.G., Frutos P., Hervás G., Gómez-Cortés P., Juárez M., de la Fuente M.A. Changes in milk fatty acid profile and animal performance in response to fish oil supplementation, alone or in combination with sunflower oil, in dairy ewes. J. Dairy Sci. 2010, 93:1604-1615.
40. Toral P.G., Hervás G., Shingfield K., Toivonen V., Belenguer A., Frutos P. Ruminal fatty acid profile and fermentation characteristics in ewes fed sunflower and fish oils. XIth International Symposium on Ruminant Physiology. Ruminant Physiology. Digestion, Metabolism, and Effects of Nutrition on Reproduction and Welfare 2009, 374-375. Y. Chilliard, F. Glasser, Y. Faulconnier, F. Bocquier, I. Veissier, M. Doreau (Eds.).
41. Van Glyswyk N.O., Wejdemar K., Kulander K. Comparative growth rates of various rumen bacteria in clarified rumen fluid from cows and sheep fed different diets. Appl. Environ. Microbiol. 1992, 58:99-105.
42. Vicini J.L., Brulla W.J., Davis C.L., Bryant M.P. Quin oval and other microbiota in the rumens of molasses-fed sheep. Appl. Environ. Microbiol. 1987, 53:1273-1276.
43. Walker A.W., Duncan S.H., McWilliam Leitch E.C., Child M.W., Flint H.J. pH and peptide supply can radically alter bacterial populations and short-chain fatty acid ratios within microbial communities from the human colon. Appl. Environ. Microbiol. 2005, 71:3692-3700.
44. Wallace R.J., Chaudhary L.C., McKain N., McEwan N.R., Richardson A., Vercoe P., Walker N., Paillard D. Clostridium proteoclasticum: A ruminal bacterium that forms stearic acid from linoleic acid. FEMS Microbiol. Lett. 2006, 265:195-201.
45. Wasowska I., Maia M.R., Niedzwiedzka K.M., Czauderna M., Ribeiro J.M., Devillard E., Shingfield K.J., Wallace R.J. Influence of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids. Br. J. Nutr. 2006, 95:1199-1211.
46. Yu Z., Morrison M. Improved extraction of PCR-quality community DNA from digesta and fecal samples. Biotechniques 2004, 36:808-812.