Isolation of nucleoli

Current Protocols in Cell Biology

Volumn , Issue SUPPL. 47, 2010, Pages

  Hacot, Sabine ^a   Coute, Yohann ^b,c   Belin, Stéphane ^a   Albaret, Marie Alexandra ^a   Mertani, Hichem C ^a   Sanchez, Jean Charles ^d   Rosa Calatrava, Manuel ^a   Diaz, Jean Jacques ^a  

^a UNIVERSITÉ DE LYON   (France)

^b CEA GRENOBLE   (France)

^c UNIV GRENOBLE ALPES   (France)

^d UNIVERSITY OF GENEVA MEDICAL SCHOOL   (Switzerland)

Author keywords

cell fractionation; nuclear domain; nucleoli

Indexed keywords

CELL PROTEIN;

CELL ADHESION; CELL FRACTIONATION; CELL POLARITY; CYTOPLASM; EPITHELIUM CELL; GEL ELECTROPHORESIS; HUMAN CELL CULTURE; NONHUMAN; NUCLEOLUS; NUCLEOLUS ISOLATION; PRIORITY JOURNAL; PROCEDURES CONCERNING CELLS; PURIFICATION; REVIEW;

ANIMALIA;

EID: 77953862481     PISSN: 19342500     EISSN: 19342616     Source Type: Journal    
DOI: 10.1002/0471143030.cb0336s47     Document Type: Review

References (34)

1. Ahmad, Y., Boisvert, F.M., Gregor, P., Cobley, A., and, Lamond, A.I., 2009. NOPdb: Nucleolar Proteome Database2008 update. Nucleic Acids Res. 37: D181-D184.
2. Andersen, J.S., Lyon, C.E., Fox, A.H., Leung, A.K., Lam, Y.W., Steen, H., Mann, M., and, Lamond, A.I., 2002. Directed proteomic analysis of the human nucleolus. Curr. Biol. 12: 1-11.
3. Andersen, J.S., Lam, Y.W., Leung, A.K., Ong, S.E., Lyon, C.E., Lamond, A.I., and, Mann, M., 2005. Nucleolar proteome dynamics. Nature 433: 77-83.
4. Brown, J.W., Shaw, P.J., Shaw, P., and, Marshall, D.F., 2005. Arabidopsis nucleolar protein database (AtNoPDB). Nucleic Acids Res. 33: D633-D636.
5. Busch, H., Muramatsu, M., Adams, H., Steele, W.J., Liau, M.C., and, Smetana, K., 1963. Isolation of nucleoli. Exp. Cell Res. 24:S9: 150-163.
6. Busch, H., Lane, M., Adams, H.R., Debakey, M.E., and, Muramatsu, M., 1965. Isolation of nucleoli from human tumors. Cancer Res. 25: 225-233.
7. Busch, H., Desjardins, R., Grogan, D., Higashi, K., Jacob, S.T., Muramatsu, M., Ro, T.S., and, Steele, W.J., 1966. Composition of nucleoli isolated from mammalian cells. J. Natl. Cancer Inst. Monogr. 23: 193-212.
8. Carter, K.C., Bowman, D., Carrington, W., Fogarty, K., McNeil, J.A., Fay, F.S., and, Lawrence, J.B., 1993. A three-dimensional view of precursor messenger RNA metabolism within the mammalian nucleus. Science 259: 1330-1335.
9. Comai, L., 1999. The nucleolus: A paradigm for cell proliferation and aging. Brazilian J. Med. Biolog. Res. 32: 1473-1478.
10. Coute, Y., Burgess, J.A., Diaz, J.J., Chichester, C., Lisacek, F., Greco, A., and, Sanchez, J.C., 2006. Deciphering the human nucleolar proteome. Mass Spectrom. Rev. 25: 215-234.
11. Dundr, M., and, Misteli, T., 2001. Functional architecture in the cell nucleus. Biochem. J. 356: 297-310.
12. Fontana, F., 1781. Trait sur le venin de la vipre. Florence.
13. Gallagher, S., Winston, S.E., Fuller, S.A., and, Hurrell, J.G.R., 2008. Immunoblotting and immunodetection. Curr. Protoc. Mol. Biol. 83: 10.8.1-10.8.28.
14. Gonzalez-Camacho, F., and, Medina, F.J., 2004. Identification of specific plant nucleolar phosphoproteins in a functional proteomic analysis. Proteomics 4: 407-417.
15. Granboulan, N., and, Granboulan, P., 1965. Ultrastructure cytochemistry of the nucleolus. II. Study of the sites of RNA synthesis in the nucleolus and the nucleus. Exp. Cell Res. 38: 604-619.
16. Henderson, A.S., Warburton, D., and, Atwood, K.C., 1972. Location of ribosomal DNA in the human chromosome complement. Proc. Natl. Acad. Sci. U.S.A. 69: 3394-3398.
17. Hiscox, J.A., 2007. RNA viruses: Hijacking the dynamic nucleolus. Nat. Rev. Microbiol. 5: 119-127.
18. Kressler, D., Linder, P., and, de La Cruz, J., 1999. Protein trans-acting factors involved in ribosome biogenesis in Saccharomyces cerevisiae. Mol. Cell Biol. 19: 7897-7912.
19. Lewis, J.D., and, Tollervey, D., 2000. Like attracts like: Getting RNA processing together in the nucleus. Science 288: 1385-1389.
20. Melese, T., and, Xue, Z., 1995. The nucleolus: An organelle formed by the act of building a ribosome. Curr. Opin. Cell Biol. 7: 319-324.
21. Montanaro, L., Trere, D., and, Derenzini, M., 2008. Nucleolus, ribosomes, and cancer. Am. J. Pathol. 173: 301-310.
22. Muramatsu, M., Smetana, K., and, Busch, H., 1963. Quantitative aspects of isolation of nucleoli of the Walker carcinosarcoma and liver of the rat. Cancer Res. 23: 510-518.
23. Ochs, R.L., 1998. Methods used to study structure and function of the nucleolus. Methods Cell Biol. 53: 303-321.
24. Olson, M.O., 2004. The Nucleolus. In Molecular Biology Intelligence Unit (R.G. Landes). Springer-verlag, Heidelberg, Germany.
25. Olson, M.O., Dundr, M., and, Szebeni, A., 2000. The nucleolus: An old factory with unexpected capabilities. Trends Cell Biol. 10: 189-196.
26. Pederson, T., 1998. The plurifunctional nucleolus. Nucleic Acids Res. 26: 3871-3876.
27. Pendle, A.F., Clark, G.P., Boon, R., Lewandowska, D., Lam, Y.W., Andersen, J., Mann, M., Lamond, A.I., Brown, J.W., and, Shaw, P.J., 2005. Proteomic analysis of the Arabidopsis nucleolus suggests novel nucleolar functions. Mol. Biol. Cell 16: 260-269.
28. Phair, R.D., and, Misteli, T., 2000. High mobility of proteins in the mammalian cell nucleus. Nature 404: 604-609.
29. Raska, I., Shaw, P.J., and, Cmarko, D., 2006. New insights into nucleolar architecture and activity. Int. Rev. Cytol. 255: 177-235.
30. Scherl, A., Coute, Y., Deon, C., Calle, A., Kindbeiter, K., Sanchez, J.C., Greco, A., Hochstrasser, D., and, Diaz, J.J., 2002. Functional proteomic analysis of human nucleolus. Mol. Biol. Cell 13: 4100-4109.
31. Simonian, M.H., and, Smith, J.A., 2006. Spectrophotometric and colorimetric determination of protein concentration. Curr. Protoc. Mol. Biol. 76: 10.1A.1-10.1A.9.
32. Sleeman, J.E., and, Lamond, A.I., 1999. Nuclear organization of pre-mRNA splicing factors. Curr. Opin. Cell Biol. 11: 372-377.
33. Stoykova, A.S., Dabeva, M.D., Dimova, R.N., and, Hadjiolov, A.A., 1985. Ribosome biogenesis and nucleolar ultrastructure in neuronal and oligodendroglial rat brain cells. J. Neurochem. 45: 1667-1676.
34. Venema, J., and, Tollervey, D., 1999. Ribosome synthesis in Saccharomyces cerevisiae. Annu. Rev. Genet. 33: 261-311.