Methods used to study structure and function of the nucleolus

Methods in Cell Biology

Volumn , Issue 53, 1998, Pages 303-321

  Ochs, Robert L ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

5,6 DICHLOROBENZIMIDAZOLE RIBOSIDE; AMANITIN; ANTIBODY; DACTINOMYCIN; NUCLEIC ACID SYNTHESIS INHIBITOR;

ANIMAL; DRUG EFFECT; HISTOLOGY; HUMAN; IMMUNOHISTOCHEMISTRY; IMMUNOLOGY; METHODOLOGY; NUCLEOLUS; PHYSIOLOGY; REVIEW; ULTRASTRUCTURE;

AMANITINS; ANIMALS; ANTIBODIES; CELL NUCLEOLUS; DACTINOMYCIN; DICHLORORIBOFURANOSYLBENZIMIDAZOLE; HISTOLOGICAL TECHNIQUES; HUMANS; IMMUNOHISTOCHEMISTRY; NUCLEIC ACID SYNTHESIS INHIBITORS;

EID: 0031961806     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: None     Document Type: Article

References (57)

1. Abelson, H. T., and Penman, S. (1975). Selective interruption of RNA metabolism by chemotherapeutic agents. Handb. Exp. Pharmakol. 38, 571-581.
2. Andrade, L. E. C, Tan, E. M., and Chan, E. K. L. (1993). Immunocytochemical analysis of the coiled body in the cell cycle and during cell proliferation. Proc. Nail. Acad. Sei. U.S.A. 90,1947-1951.
3. Brasch, K. (1990). Drug and metabolite-induced perturbations in nuclear structure and function: A review. Biochem. Cell Biol. 68, 408-426.
4. Brasch, K., and Ochs, R. L. (1992). Nuclear bodies (NBs): A newly "rediscovered" organelle. Exp. Cell Res. 202,211-223
5. Busch, H., and Smetana, K. (1970). "The Nucleolus." Academic Press, New York.
6. Chan, E. K. L., Imai, H., Hamel, J. C., and Tan, E. M. (1991). Human autoantibody to RNA polymerase I transcription factor hUBF. Molecular identity of nucleolus organizer region autoantigen NOR-90 and ribosomal RNA upstream binding factor. J. Exp. Med. 173,1239-1244.
7. Crocker, J. (1990). Nucleolar organizer regions. Cttrr. Top. Pathol. 82, 91-149.
8. Derenzini, M., and Ploton, D. (1991). Interphase nucleolar organizer regions in cancer cells. Int. Rev. Exp. Pathol. 32, 149-192.
9. Derenzini, M., Pession, A., and Trerè, D. (1990). Quantity of nucleolar silver-stained proteins is related to proliferating activity in cancer cells. Lab. Invest. 63, 137-140.
10. Derenzini, M., Sirri, V., and Trerè, D. (1994). Nucleolar organizer regions in tumor cells. Cancer J. 7, 71-77.
11. Derenzini, M., Sirri, V., Pession, A., Trerè, D., Roussel, P., Ochs, R. L., and Hernandez-Verdun, D. (1995a). Quantitative changes of the two major AgNOR proteins, nucleolin and protein B23, related to stimulation of rDNA transcription. Exp. Cell Res. 219, 276-282.
12. Derenzini, M., Sirri, V., Trerè, D., and Ochs, R. L. (1995b). The quantity of nucleolar proteins nucleolin and protein B23 is related to cell doubling time in human cancer cells. Lab. Invest. 73, 497-502.
13. Goessens, G. (1984). Nucleolar structure. //;J. Rev. Cytol. 87, 107-158.
14. Granick, D. (1975a). Nucleolar necklaces in chick embryo fibroblast cells. I. Formation of necklaces by dichlororibobenzimidazole and other adenosine analogues that decrease RNA synthesis and degrade preribosomes. J. Cell Biol. 65, 389-417.
15. Granick, D. (1975b). Nucleolar necklaces in chick embryo fibroblast cells. II. Microscope observations of the effect of adenosine analogues on nucleolar necklace formation. J. Cell Biol. 65,418-427.
16. Hadjiolov, A. A. (1985). The nucleolus and ribosome biogenesis. In "Cell Biology Monographs," Vol. 12, pp. 1-263. Springer-Verlag, New York.
17. Howell, W. M. (1982). Selective staining of nucleolus organizer regions (NORs). In "The Cell Nucleus" (H. Busch and L. Rothblum, eds.), Vol. 11, Part B, pp. 89-142. Academic Press, New York and London.
18. Howell, W. M., and Black, D. A. (1980). Controlled silver staining of nucleolus organizer regions with a protective colloidal developer: A one-step method. Experientia 36, 1014.
19. Hozâk, P., Roussel, P., and Hernandez-Verdun, D. (1992). Procedures for specific detection of silver-stained nucleolar proteins on Western blots. J. Histochem. Cytochem. 40, 1089-1096.
20. Hozâk, P., Cook, P. R., Schhofer, C., Mosgoller, W., and Wachtier, F. (1994). Site of transcription of ribosomal RNA and intranucleolar structure in HeLa cells. J. Cell Sei. 107, 639-648.
21. Jiménez-Garcîa, L. F., Rothblum, L. L, Busch, H., and Ochs, R. L. (1989). Nucleologenesis: Use of non-isotopic in situ hybridization and immunocytochemistry to compare the localization of rDNA and nucleolar proteins during mitosis. Biol. Cell. 65, 239-246.
22. Jimenez-Garcfa, L. F., Segura-Valdez, M. de L., Ochs, R. L., Rothblum, L. L, Hannan, R., and Spector, D. L. (1994). Nucleologenesis: U3 snRNA-containing prenudeolar bodies move to sites of active pre-rRNA transcription after mitosis. Mol. Biol. Cell 5, 955-966.
23. Jordan, E. G. (1984). Nucleolar nomenclature. J. Cell Sei. 67, 217-220.
24. Jordan, E. G., and McGovern, J. H. (1981). The quantitative relationship of the fibrillar centres and other nucleolar components in changes in growth conditions, serum deprivation and low doses of actinomycin D in cultured diploid human fibroblasts (strain MRC-6). J. Cell Sei. 52, 373-389.
25. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227, 680-685.
26. Lamond, A. I., and Carmo-Fonseca, M. (1993). The coiled body. Trends Cell Biol. 3, 198-204.
27. Meier, U. T., and Blobel, G. (1992). Nopp 140 shuttles on tracks between nucleolus and cytoplasm. Cell (Cambridge, Mass.) 70,127-138.
28. Meier, U. T., and Blobel, G. (1994). NAP57, a mammalian nucleolar protein with a putative homolog in yeast and bacteria. J. Cell. Biol. 127, 1505-1514.
29. Muramatsu, M., Hayashi, Y., Onishi, T., Sakai, M., Takai, K., and Kashiyama, T. (1974). Rapid isolation of nucleoli from detergent purified nuclei of various tumor and tissue culture cells. Exp. Cell Res. 88, 345-351.
30. Ochs, R. L., and Press, R. I. (1992). Centromere autoantigens are associated with the nucleolus. Exp. Cell Res. 200, 339-350.
31. Ochs, R. L., and Smetana, K. (1989). Fibrillar center distribution in nucleoli of PHA-stimulated human lymphocytes. Exp. Cell Res. 184, 552-557.
32. Ochs, R. L., Lischwe, M. A., Shen, E., Carrol, R. E., and Busch, H. (1985a). Nucleologenesis: composition and fate of prenucleolar bodies. Chromosoma 92, 330-336.
33. Ochs, R. L., Lischwe, M. A., Spohn, W. H., and Busch, H. (1985b). Fibrillarin: A new protein of the nucleolus identified by autoimmune sera. Biol. Cell. 54, 123-134.
34. Ochs, R. L., Stein, T. W., Jr., and Tan, E. M. (1994). Coiled bodies in the nucleolus of breast cancer cells. J. Cell Sei. 107, 385-399.
35. Ochs, R. L., Stein, T. W., Jr., Chan, E. K. L., Ruutu, M., and Tan E. M. (1996). cDNA cloning and characterization of a novel nucleolar protein. Mol. Biol. Cell 7, 1015-1024.
36. Oison, M. O. J. (1990). The role of proteins in nucleolar structure and function. In "The Eukaryotic Nucleus" (P. R. Strauss and S. H. Wilson, eds.), Vol. 2, pp. 519-559. Telford Press, Caldwell, NJ.
37. Ploton, D., Menager, M., Jeannesson, P., Himber, G., Pigeon, F., and Adnet, J. J. (1986). Improvement in the staining and in the visualization of the argyrophilic proteins of the nucleolar organizer region at the optical level. Histochem J. 18, 5-14.
38. Ramön y Cajâl, S. (1903). Un sencillo metodo de coloracion del reticulo protoplasmico y sus efectos en los diversos organos nerviosos. Trab. Lab. Invest. Biol. 2, 129-221.
39. Raska, I., Ochs, R. L., Andrade, L. E. C, Chan, E. K. L., Burlingarae, R., Peebles, C. Gruol, D., and Tan, E. M. (1990a). Association between the nucleolus and the coiled body. J. Struct. Biol. 104, 120-127.
40. Raska, L, Ochs, R. L., and Salamin-Michel, L. (1990b). Immunocytochemistry of the cell nucleus. Electron Microsc. Rev. 3, 301-353.
41. Raska, L, Andrade, L. E. C, Ochs, R. L., Chan, E. K. L., Chang, C. M., Roos, G., and Tan, E. M. (1991). Immunological and ultrastructural studies of the nuclear coiled body with autoimmune antibodies. Exp. Cell Res. 195, 27-37.
42. Raska, I., Dundr, M., Koberna, K., Melcak, I., Risueno, M., and Torok, I. (1995). Does the synthesis of ribosomal RNA take place within nucleolar fibrillar centers or dense fibrillar components? A critical appraisal. J. Struct. Biol. 114, 1-22.
43. Reimer, G., Raska, I., Tan, E. M. and Scheer, U. (1987). Human autoantibodies: Probes for nucleolus structure and function. Virchows Arch. B 54, 131-143.
44. Rendon, M. C., Rodrigo, R. M., Goenechea, L. G., Garcia-Herdugo, G., Valdivia, M. M., and Moreno, F. J. (1992). Characterization and immunolocalization of a nucleolar antigen with anti-NOR serum in HeLa cells. Exp. Cell Res. 200, 393-403.
45. Rodrigo, R. M., Rendon, M. C., Torreblanca, J., Garcia-Herdugo, G., and Moreno, F. J. (1992). Characterization and immunolocalization of RNA polymerase I transcription factor DBF with anti-NOR serum in protozoa, higher plant and vertebrate cells. J. Cell Sei. 103, 1053-1063.
46. Roussel, P., and Hernandez-Verdun, D. (1994). Identification of Ag-NOR proteins, markers of proliferation related to ribosomal gene activity. Exp. Cell Res. 214, 465-472.
47. Roussel, P., Andre, C., Masson, C., Geraud, G., and Hernandez-Verdun, D. (1993). Localization of the RNA polymerase I transcription factor hUBF during the cell cycle. J. Cell Sei. 104, 327-337.
48. Scheer, U., and Rose, K. M. (1984). Localization of RNA polymerase I in interphase cells and mitotic chromosomes by light and electron microscopic immunocytochemistry. Proc. Natl. Acad. Sei. U.S.A. 81, 1431-1435.
49. Scheer, U., Hügle, B., Kazan, R., and Rose, K. M. (1984). Drug-induced dispersal of transcribed rRNA genes and transcriptional products: Immunolocalization and silver staining of different nucleolar components in rat cells treated with 5,6-dichloro-D-ribofuranosylbenzimidazole. J. Cell Biol. 99, 672-679.
50. Shaw, P. J., and Jordan, E. G. (1995). The nucleolus. Annu. Rev. Cell Dev. Biol. 11, 93-121.
51. Simard, R., and Bernhard, W. (1966). The phenomenon of nucleolar segregation: Specific action of certain antimetabolites. Int. J. Cancer 1, 463-479
52. Smetana, K. (1967). Basic histochemical and cytochemical methods. Methods Cancer Res. 2,361-443.
53. Spector, D. L. (1993). Macromolecular domains within the cell nucleus. Annu. Rev. Cell Biol. 9, 265-315.
54. Spector, D. L., Ochs, R. L., and Busch, H. (1984). Silver staining, immunofluorescence, and immunoelectron microscopic sublocalization of nucleolar phosphoproteins B23 and C23: Functional implications. Chromosoma 90, 139-148.
55. Towbin, H., Staehelin, T., and Gordon, J. (1979). Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sei. U.S.A. 76. 4350-4354.
56. Wachtier, F., Ellinger, A., and Schwarzacher, H. G. (1980). Nucleolar changes in human phytohaemagglutinin-stimulated lymphocytes. Cell Tissue Res. 213, 351-360.
57. Yung, B.Y., Busch, H., and Chan, P. K. (1985). Translocation of nucleolar phosphoprotein B23 (37 kDa/pI 5.1) induced by selective inhibitors of ribosome synthesis. Biochim. Biopliys. Acla 826, 167-173.