Humanized culture medium for clinical expansion of human erythroblasts

Cell Transplantation

Volumn 19, Issue 4, 2010, Pages 453-469

  Migliaccio, Giovanni ^a,b   Sanchez, Massimo ^b   Masiello, Francesca ^b   Tirelli, Valentina ^b   Varricchio, Lilian ^a   Whitsett, Carolyn ^a   Migliaccio, Anna Rita ^a,b  

^a Tisch Cancer Institute   (United States)

^b ISTITUTO SUPERIORE DI SANITÀ   (Italy)

Author keywords

Erythroblasts; Growth factors; Human albumin; Human erythroid massive amplification (HEMA) culture; Transfusion

Indexed keywords

DEXAMETHASONE; ESTRADIOL; GROWTH FACTOR; INTERLEUKIN 3; STEM CELL FACTOR; THROMBOPOIETIN; CULTURE MEDIUM; ERYTHROPOIETIN;

ARTICLE; CELL COUNT; CELL EXPANSION; CELL STRUCTURE; CELL VIABILITY; ERYTHROBLAST; EX VIVO STUDY; HUMAN; IMMUNOPHENOTYPING; MONONUCLEAR CELL; PRIORITY JOURNAL; CELL CULTURE; CULTURE MEDIUM; CYTOLOGY; PHARMACOLOGY;

BOVINAE;

CELLS, CULTURED; CULTURE MEDIA; DEXAMETHASONE; ERYTHROBLASTS; ERYTHROPOIETIN; ESTRADIOL; HUMANS; INTERLEUKIN-3; STEM CELL FACTOR; THROMBOPOIETIN;

EID: 77953733401     PISSN: 09636897     EISSN: None     Source Type: Journal    
DOI: 10.3727/096368909X485049     Document Type: Article

References (58)

1. Baek, E. J.; Kim, H. S.; Kim, S.; Jin, H.; Choi, T. Y.; Kim, H. O. In vitro clinical-grade generation of red blood cells from human umbilical cord blood CD34+ cells. Transfusion 48:2235-2245; 2008.
2. Bartolovic, K.; Balabanov, S.; Berner, B.; Buhring, H. J.; Komor, M.; Becker, S.; Hoelzer, D.; Kanz, L.; Hofmann, W. K.; Brummendorf, T. H. Clonal heterogeneity in growth kinetics of CD34+CD38- human cord blood cells in vitro is correlated with gene expression pattern and telomere length. Stem Cells 23:946-957; 2005.
3. Bauer, A.; Tronche, F.; Wessely, O.; Kellendonk, C.; Reichardt, H. M.; Steinlein, P.; Schutz, G.; Beug, H. The glucocorticoid receptor is required for stress erythropoiesis. Genes Dev. 13:2996-3002; 1999.
4. Bauer, A.; Ulrich, E.; Andersson, M.; Beug, H.; von Lindern, M. Mechanism of transformation by v-ErbA: substitution for steroid hormone receptor function in self renewal induction. Oncogene 15:701-715; 1997.
5. Belgi, G.; Friedmann, P. S. Traditional therapies: Glucocorticoids, azathioprine, methotrexate, hydroxyurea. Clin. Exp. Dermatol. 27:546-554; 2002.
6. Blobel, G. A.; Orkin, S. H. Estrogen-induced apoptosis by inhibition of the erythroid transcription factor GATA-1. Mol. Cell. Biol. 16:1687-1694; 1996.
7. Calado, R. T.; Yewdell, W. T.; Wilkerson, K. L.; Regal, J. A.; Kajigaya, S.; Stratakis, C. A.; Young, N. S. Sex hormones, acting on the TERT gene, increase telomerase activity in human primary hematopoietic cells. Blood 11: 2236-2243; 2009.
8. Chang, T. J.; Scher, B. M.; Waxman, S.; Scher, W. Inhibition of mouse GATA-1 function by the glucocorticoid receptor: Possible mechanism of steroid inhibition of erythroleukemia cell differentiation. Mol. Endocrinol. 7: 528-542; 1993.
9. Costa, E.; Lima, M.; Alves, J. M.; Rocha, S.; Rocha-Pereira, P.; Castro, E.; Miranda, V.; do Sameiro Faria, M.; Loureiro, A.; Quintanilha, A.; Belo, L.; Santos-Silva, A. Inflammation, T-cell phenotype, and inflammatory cyto kines in chronic kidney disease patients under hemodialysis and its relationship to resistance to recombinant human erythropoietin therapy. J. Clin. Immunol. 28:268-275; 2008.
10. De Feo, T. M.; Cappellini, M. D.; Fiorelli, G. Effect of estrogens and progesterone on human peripheral erythroid burst-forming unit (BFU-E) growth. Am. J. Hematol. 38: 81-85; 1991.
11. DeRijk, R.; de Kloet, E. R. Corticosteroid receptor genetic polymorphisms and stress responsivity. Endocrine 28: 263-270; 2005.
12. Dorn, I.; Lazar-Karsten, P.; Boie, S.; Ribbat, J.; Hartwig, D.; Driller, B.; Kirchner, H.; Schlenke, P. In vitro proliferation and differentiation of human CD34+ cells from peripheral blood into mature red blood cells with two different cell culture systems. Transfusion 48:1122-1132; 2008.
13. Dukes, P. P.; Goldwasser, E. Inhibition of erythropoiesis by estrogens. Endocrinology 69:21-29; 1961.
14. Edvardsson, L.; Dykes, J.; Olofsson, T. Isolation and characterization of human myeloid progenitor populations - TpoR as discriminator between common myeloid and megakaryocyte/erythroid progenitors. Exp. Hematol. 34: 599-609; 2006.
15. Eliason, J. F.; Odartchenko, N. Colony formation by primitive hemopoietic progenitor cells in serum-free medium. Proc. Natl. Acad. Sci. USA 82:775-779; 1985.
16. FDA guidelines for cell therapy. Available at http://www.fda.gov/cber/ ind/ind.htm
17. Giarratana, M. C.; Kobari, L.; Lapillonne, H.; Chalmers, D.; Kiger, L.; Cynober, T.; Marden, M. C.; Wajcman, H.; Douay, L. Ex-vivo generation of fully mature human red blood cells from hematopoietic stem cells. Nat. Biotechnol. 23:69-74; 2005.
18. Golde, D. W.; Bersch, N.; Cline, M. J. Potentiation of erythropoiesis in vitro by dexamethasone. J. Clin. Invest. 57:57-62; 1976.
19. Gursoy, A.; Dogruk Unal, A.; Ayturk, S.; Karakus, S.; Nur Izol, A.; Bascil Tutuncu, N.; Guvener Demirag, N. Polycythemia as the first manifestation of Cushing's disease. J. Endocrinol. Invest. 29:742-744; 2006.
20. Hayflick, L. The future of ageing. Nature 408:267-269; 2000.
21. Hayflick, L.; Moorhead, P. S. The serial cultivation of human diploid cell strains. Exp. Cell Res. 25:585-621; 1961.
22. Hills, M.; Lucke, K.; Chavez, E. A.; Eaves, C. J.; Lansdorp, P. M. Probing the mitotic history and developmental stage of hematopoietic cells using single telomere length analysis (STELA). Blood 113:5765-5775; 2009.
23. Iscove, N. N.; Guilbert, L. J.; Weyman, C. Complete replacement of serum in primary cultures of erythropoietindependent red cell precursors (CFU-E) by albumin, trans- ferrin, iron, unsaturated fatty acid, lecithin and cholesterol. Exp. Cell Res. 126:121-126; 1980.
24. Kaushansky, K. Lineage-specific hematopoietic growth factors. N. Engl. J. Med. 354:2034-2045; 2006.
25. Kolbus, A.; Blazquez-Domingo, M.; Carotta, S.; Bakker, W.; Luedemann, S.; von Lindern, M.; Steinlein, P.; Beug, H. Cooperative signaling between cytokine receptors and the glucocorticoid receptor in the expansion of erythroid progenitors: Molecular analysis by expression profiling. Blood 102:3136-3146; 2003. (Pubitemid 37314749)
26. Kuo, Y. Y.; Chang, Z. F. GATA-1 and Gfi-1B interplay to regulate Bcl-xL transcription. Mol. Cell. Biol. 27: 4261-4272; 2007.
27. Leberbauer, C.; Boulme, F.; Unfried, G.; Huber, J.; Beug, H.; Mullner, E. W. Different steroids co-regulate long-term expansion versus terminal differentiation in primary human erythroid progenitors. Blood 105:85-94; 2005.
28. Leimberg, J. M.; Konijn, A. M.; Fibach, E. Developing human erythroid cells grown in transferrin-free medium utilize iron originating from extracellular ferritin. Am. J. Hematol. 73:211-212; 2003.
29. Lu, L.; Pelus, L. M.; Broxmeyer, H. E. Modulation of the expression of HLA-DR (Ia) antigens and the proliferation of human erythroid (BFU-E) and multipotential (CFUGEMM) progenitor cells by prostaglandin E. Exp. Hematol. 12:741-748; 1984.
30. Migliaccio, A. R.; Whitsett, C.; Migliaccio, G. Erythroid cells in vitro: from developmental biology to blood transfusion products. Curr. Opin. Hematol. 16:259-268; 2009.
31. Migliaccio, G.; Di Pietro, R.; di Giacomo, V.; Di Baldassarre, A.; Migliaccio, A. R.; Maccioni, L.; Galanello, R.; Papayannopoulou, T. In vitro mass production of human erythroid cells from the blood of normal donors and of thalassemic patients. Blood Cells Mol. Dis. 28:169-180; 2002.
32. Migliaccio, G.; Migliaccio, A. R. Cloning of human erythroid progenitors (BFU-E) in the absence of fetal bovine serum. Br. J. Haematol. 67:129-133; 1987.
33. Migliaccio, G.; Migliaccio, A. R.; Adamson, J. W. The biology of hematopoietic growth factors: Studies in vitro under serum-deprived conditions. Exp. Hematol. 18:1049-1055; 1990.
34. Migliaccio, G.; Migliaccio, A. R.; Druzin, M. L.; Giardina, P. J.; Zsebo, K. M.; Adamson, J. W. Long-term generation of colony-forming cells in liquid culture of CD34+ cord blood cells in the presence of recombinant human stem cell factor. Blood 79:2620-2627; 1992.
35. Migliaccio, G.; Sanchez, M.; LeBlanc, A.; Masiello, F.; Tirelli, V.; Migliaccio, A. R.; Najfeld, V.; Whitsett, C. Long-term storage does not alter functionality of in vitro generated human erythroblasts: Implications for ex-vivo generated erythroid transfusion products. Transfusion 49: 2668-2679; 2009.
36. Miharada, K.; Hiroyama, T.; Sudo, K.; Nagasawa, T.; Nakamura, Y. Efficient enucleation of erythroblasts differentiated in vitro from hematopoietic stem and progenitor cells. Nat. Biotechnol. 24:1255-1256; 2006.
37. Narla, M. Banking on red blood cells. Nat. Biotechnol. 23:35-36; 2005.
38. Orkin, S. H.; Zon, L. I. Hematopoiesis: An evolving paradigm for stem cell biology. Cell 132:631-644; 2008.
39. Panzenbock, B.; Bartunek, P.; Mapara, M. Y.; Zenke, M. Growth and differentiation of human stem cell factor/ erythropoietin-dependent erythroid progenitor cells in vitro. Blood 92:3658-3668; 1998.
40. Papayannopoulou, T.; Abkowitz, J.; D'Andrea, A.; Migliaccio, A. R. Biology of erythtropoiesis, erythroid differentiation and maturation. In: Hoffman, R.; Benz, E. J.; Shattil, S. J.; Furie, B.; Silberstein, L. E.; McGlave, P.; Heslop, H., eds. Hematology: Basic principles and practice, 5th ed. Philadelphia, PA: Elsevier; 2009:276-294.
41. Peoples, J. D.; Cheung, S.; Nesin, M.; Lin, H.; Tatad, A. M.; Hoang, D.; Perlman, J. M.; Cunningham-Rundles, S. Neonatal cord blood subsets and cytokine response to bacterial antigens. Am. J. Perinatol. 9:647-657; 2009.
42. Piacibello, W.; Sanavio, F.; Garetto, L.; Severino, A.; Bergandi, D.; Ferrario, J.; Fagioli, F.; Berger, M.; Aglietta, M. Extensive amplification and self-renewal of human primitive hematopoietic stem cells from cord blood. Blood 89:2644-2653; 1997. (Pubitemid 27172548)
43. Prade-Houdellier, N.; Frebet, E.; Demur, C.; Gautier, E. F.; Delhommeau, F.; Bennaceur-Griscelli, A. L.; Gaudin, C.; Martinel, V.; Laurent, G.; Mansat-De Mas, V.; Beyne-Rauzy, O. Human telomerase is regulated by erythropoietin and transforming growth factor-beta in human erythroid progenitor cells. Leukemia 21:2304-2310; 2007.
44. Ribeil, J. A.; Zermati, Y.; Vandekerckhove, J.; Cathelin, S.; Kersual, J.; Dussiot, M.; Coulon, S.; Moura, I. C.; Zeuner, A.; Kirkegaard-Sorensen, T.; Varet, B.; Solary, E.; Garrido, C.; Hermine, O. Hsp70 regulates erythropoiesis by preventing caspase-3-mediated cleavage of GATA- 1. Nature 445:102-105; 2007.
45. Schuller, C. E.; Jankowski, K.; Mackenzie, K. L. Telomere length of cord blood-derived CD34(+) progenitors predicts erythroid proliferative potential. Leukemia 21: 983-991; .
46. Selvaggi, T. A.; Walker, R. E.; Fleisher, T. A. Development of antibodies to fetal calf serum with arthus-like reactions in human immunodeficiency virus-infected patients given syngeneic lymphocyte infusions. Blood 89: 776-779; 1997.
47. Shapiro, H. M. Practical flow cytometry, 4th ed. New York: Wiley-Liss; 2003.
48. Singer, J. W.; Adamson, J. W. Steroids and hematopoiesis III. The response of granulocytic and erythroid colony-forming cells to steroids of different classes. Blood 48: 855-864; 1976.
49. Stellacci, E.; Di Noia, A.; Di Baldassarre, A.; Migliaccio, G.; Battistini, A.; Migliaccio, A. R. Interaction between the glucocorticoid and erythropoietin receptors in human erythroid cells. Exp. Hematol. 37:559-572; 2009.
50. Stewart, S.; Zhu, B.; Axelrad, A. A "serum-free" medium for the production of erythropoietic bursts by murine bone marrow cells. Exp. Hematol. 12:309-318; 1984.
51. Takakura, N.; Ogawa, M.; Kodama, H.; Nishikawa, S.; Nishikawa, S. Self-renewal of hematopoietic progenitor cells under defined condition. Leukemia 11(Suppl. 3):460; 1997.
52. Tsiftsoglou, A. S.; Wong, W.; Housman, D. E. Dexamethasone-sensitive and -insensitive responses during in vitro differentiation of Friend erythroleukemia cells. Biochim. Biophys. Acta 759:160-169; 1983.
53. Vicent, G. P.; Ballare, C.; Zaurin, R.; Saragueta, P.; Beato, M. Chromatin remodeling and control of cell proliferation by progestins via cross talk of progesterone receptor with the estrogen receptors and kinase signaling pathways. Ann. NY Acad. Sci. 1089:59-72; 2006.
54. von Lindern, M.; Zauner, W.; Mellitzer, G.; Steinlein, P.; Fritsch, G.; Huber, K.; Lowenberg, B.; Beug, H. The glucocorticoid receptor cooperates with the erythropoietin receptor and c-Kit to enhance and sustain proliferation of erythroid progenitors in vitro. Blood 94:550-559; 1999.
55. Wessely, O.; Deiner, E. M.; Beug, H.; von Lindern, M. The glucocorticoid receptor is a key regulator of the decision between self-renewal and differentiation in erythroid progenitors. EMBO J. 16:267-280; 1997.
56. Whyatt, D.; Lindeboom, F.; Karis, A.; Ferreira, R.; Milot, E.; Hendriks, R.; de Bruijn, M.; Langeveld, A.; Gribnau, J.; Grosveld, F.; Philipsen, S. An intrinsic but cell-nonautonomous defect in GATA-1-overexpressing mouse erythroid cells. Nature 406:519-524; 2000.
57. Xiaosong, H.; Pierce, L. J.; Cobine, P. A.; Winge, D. R.; Spangrude, G. J. Copper modulates the differentiation of mouse hematopoietic progenitor cells in culture. Cell Transplant. 18:887-897; 2009.
58. Ziegler, B. L.; Muller, R.; Valtieri, M.; Lamping, C. P.; Thomas, C. A.; Gabbianelli, M.; Giesert, C.; Buhring, H. J.; Kanz, L.; Peschle, C. Unicellular-unilineage erythropoietic cultures: Molecular analysis of regulatory gene expression at sibling cell level. Blood 93:3355-3368; 1999. (Pubitemid 29220903)