Developing human erythroid cells grown in transferrin-free medium utilize iron originating from extracellular ferritin

American Journal of Hematology

Volumn 73, Issue 3, 2003, Pages 211-212

  Leimberg, J M ^a   Konijn, A M ^a   Fibach, E ^b  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^b HADASSAH HEBREW UNIVERSITY MEDICAL CENTER   (Israel)

Author keywords

Erythroid precursors; Ferritin; Hemoglobin; Iron; Transferrin

Indexed keywords

DEXAMETHASONE; FERRITIN; HEME; HEMOGLOBIN; IRON; RECOMBINANT ERYTHROPOIETIN; TRANSFERRIN;

ARTICLE; BIOSYNTHESIS; CELL CULTURE; CELL GROWTH; CELL PROLIFERATION; COLONY FORMING UNIT E; CULTURE MEDIUM; ERYTHROID CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL;

CELL CULTURE TECHNIQUES; CULTURE MEDIA, SERUM-FREE; ERYTHROID PROGENITOR CELLS; FEMALE; FERRITINS; HUMANS; IRON; PLACENTA; PREGNANCY; REFERENCE VALUES; TRANSFERRIN;

EID: 0037969721     PISSN: 03618609     EISSN: None     Source Type: Journal    
DOI: 10.1002/ajh.10355     Document Type: Article

References (8)

1. Gelvan D, Fibach E, Meyron-Holtz EG, Konijn AM. Ferritin uptake by human erythroid precursors is a regulated iron uptake pathway. Blood 1996;88:3200-3207.
2. Meyron-Holtz EG, Vaisman B, Cabantchik ZI, et al. Regulation of intracellular iron metabolism in human erythroid precursors by internalized extracellular ferritin. Blood 1999;94:3205-3211.
3. van Renswoude J, Bridges KR, Harford JB, Klausner RD. Receptor-mediated endocytosis of transferrin and the uptake of Fe in K562 cells: identification of a nonlysosomal acidic compartment. Proc Natl Acad Sci U S A 1982;79:6186-6190.
4. Vaisman B, Fibach E, Konijn AM. Utilization of intracellular ferritin iron for hemoglobin synthesis in developing human erythroid precursors. Blood 1997;90:831-838.
5. Fibach E, Manor D, Oppenheim A, Rachmilewitz EA. Proliferation and maturation of human erythroid progenitors in liquid culture. Blood 1989;73:100-103.
6. Konijn AM, Tal R, Levy R, Matzner Y. Isolation and fractionation of ferritin from human term placenta - a source for human isoferritins. Anal Biochem 1985;144:423-428.
7. Orkin SH, Harosi FI, Leder P. Differentiation in erythroleukemic cells and their somatic hybrids. Proc Natl Acad Sci U S A 1975;72:98-102.
8. Fibach E. Measurement of total and fetal hemoglobin in cultured human erythroid cells by a novel micromethod. Hemoglobin 1993;17: 41-53.