Fluorescent in situ hybridization in combination with filter cultivation (FISHFC) method for specific detection and enumeration of viable Clostridium perfringens

Food Microbiology

Volumn 26, Issue 4, 2009, Pages 425-431

  Shimizu, S ^a   Ootsubo, M ^b   Kubosawa, Y ^a   Fuchizawa, I ^a   Kawai, Y ^a   Yamazaki, K ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

^b HOKKAIDO INDUSTRIAL TECHNOLOGY CENTER   (Japan)

Author keywords

Clostridium perfringens; Fluorescent in situ hybridization; Specific detection

Indexed keywords

RNA 16S;

ARTICLE; BACTERIAL COUNT; CHEMISTRY; CLOSTRIDIUM PERFRINGENS; FLUORESCENCE IN SITU HYBRIDIZATION; FOOD CONTAMINATION; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROPORE FILTER; SAFETY; SENSITIVITY AND SPECIFICITY; SPECIES DIFFERENCE; STANDARD; TIME;

CLOSTRIDIUM PERFRINGENS; COLONY COUNT, MICROBIAL; CONSUMER PRODUCT SAFETY; FOOD CONTAMINATION; IN SITU HYBRIDIZATION, FLUORESCENCE; MICROPORE FILTERS; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY; TIME FACTORS;

CLOSTRIDIUM PERFRINGENS;

EID: 64449088788     PISSN: 07400020     EISSN: 10959998     Source Type: Journal    
DOI: 10.1016/j.fm.2009.02.002     Document Type: Article

References (40)

1. Adcock P.W., and Saint C.P. Rapid confirmation of Clostridium perfringens by using chromogenic and fluorogenic substrates. Appl. Environ. Microbiol. 67 (2001) 4382-4384
2. Alonso J.L., Soriano A., Carbajo O., Amoros I., and Garelick H. Comparison and recovery of Escherichia coli and thermotolerant coliforms in water with a chromogenic medium incubated at 41 and 44.5 °C. Appl. Environ. Microbiol. 65 (1999) 3746-3749
3. Amann R.I., Krumholz L., and Stahl D.A. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J. Bacteriol. 172 (1990) 762-770
4. Amann R.I., Ludwig W., and Schleifer K.H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59 (1995) 143-169
5. Ando Y., Tsuzuki T., and Sunagawa H. Heat resistance, spore germination, and enterotoxigenicity of Clostridium perfringens. Microbiol. Immunol. 29 (1985) 317-326
6. Araujo M., Sueiro R.A., Gómez M.J., and Garrido M.J. Enumeration of Clostridium perfringens spores in groundwater samples: comparison of six culture media. J. Microbiol. Methods 57 (2004) 175-180
7. Bottari B., Ercolini D., Gatti M., and Neviani E. Application of FISH technology for microbiological analysis: current state and prospects. Appl. Microbiol. Biotechnol. 73 (2006) 485-494
8. Collins M.D., Lawson P.A., Willems A., Cordoba J.J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., and Farrow J.A. The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int. J. Syst. Bacteriol. 44 (1994) 812-826
9. Fallani M., Rigottier-Gois L., Aguilera M., Bridonneau C., Collignon A., Edwards C.A., Corthier G., and Doré J. Clostridium difficile and Clostridium perfringens species detected in infant faecal microbiota using 16S rRNA targeted probes. J. Microbiol. Methods 67 (2006) 150-161
10. Franks A.H., Harmsen H.J., Raangs G.C., Jansen G.J., Schut F., and Welling G.W. Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes. Appl. Environ. Microbiol. 64 (1998) 3336-3345
11. Fuchizawa I., Shimizu S., Kawai Y., and Yamazaki K. Specific detection and quantitative enumeration of Listeria spp. using fluorescent in situ hybridization in combination with filter cultivation (FISHFC). J. Appl. Microbiol. 105 (2008) 502-509
12. Gendel S.M. Computational analysis of the specificity of 16S rRNA-derived signature sequences for identifying food-related microbes. Food Microbiol. 13 (1996) 1-15
13. Gurjar A.A., Hegde N.V., Love B.C., and Jayarao B.M. Real-time multiplex PCR assay for rapid detection and toxintyping of Clostridium perfringens toxin producing strains in feces of dairy cattle. Mol. Cell. Probes 22 (2008) 90-95
14. Harmsen H.J., Gibson G.R., Elfferich P., Raangs G.C., Wildeboer-Veloo A.C., Argaiz A., Roberfroid M.B., and Welling G.W. Comparison of viable cell counts and fluorescence in situ hybridization using specific rRNA-based probes for the quantification of human fecal bacteria. FEMS Microbiol. Lett. 183 (2000) 125-129
15. Hauschild A.H., and Hilsheimer R. Enumeration of food-borne Clostridium perfringens in egg yolk-free tryptose-sulfite-cycloserine agar. Appl. Microbiol. 27 (1974) 521-526
16. Hesselsøe M., Brandt K.K., and Sørensen J. Quantification of ammonia oxidizing bacteria in soil using microcolony technique combined with fluorescence in situ hybridization (MCFU-FISH). FEMS Microbiol. Ecol. 38 (2001) 87-95
17. Jong A.E.I., Eijhusen G.P., Brouwer-Post E.J.F., Grand M., Johansson T., Karkkainen T., Marugg J., Veld P.H., Warmerdam F.H.M., Worner G., Zicavo A., Rombouts F.M., and Beumer R.R. Comparison of media for enumeration of Clostridium perfringens from foods. J. Microbiol. Methods 54 (2003) 359-366
18. Jose S.G.-A., Labbe R.G., and Manuel A.R. Sporulation and enterotoxin production by Clostridium perfringens Type A at 37 and 43 °C. Appl. Environ. Microbiol. 58 (1992) 1411-1414
19. Kepner R.L., and Pratt J.R. Use of fluorochromes for direct enumeration of total bacteria in environmental samples: past and present. Microbiol. Rev. 58 (1994) 603-615
20. Kerkhof L., and Kemp P. Small ribosomal RNA content in marine Proteobacteria during non-steady-state growth. FEMS Microbiol. Ecol. 30 (1999) 253-260
21. Koonjul P.K., Brandt W.F., Farrant J.M., and Lindsey G.G. Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA. Nucleic Acids Res. 27 (1999) 915-916
22. Krimmer V., Merkert H., von Eiff C., Frosch M., Eulert J., Löhr J.F., Hacker J., and Ziebuhr W. Detection of Staphylococcus aureus and Staphylococcus epidermidis in clinical samples by 16S rRNA-directed in situ hybridization. J. Clin. Microbiol. 37 (1999) 2667-2673
23. McCourt M.T., Finlay D.A., Laird C., Smyth J.A., Bell C., and Ball H.J. Sandwich ELISA detection of Clostridium perfringens cells and alpha-toxin from field cases of necrotic enteritis of poultry. Vet. Microbiol. 106 (2005) 259-264
24. McClane B.A. The complex interactions between Clostridium perfringens enterotoxin and epithelial tight junctions. Toxicon 39 (2001) 1781-1791
25. McDonel J.L. Clostridium perfringens toxins (type A, B, C, D, E). Pharmacol. Ther. 10 (1980) 617-655
26. Moter A., and Göbel U.B. Fluorescence in situ hybridization (FISH) for direct visualization of microorganisms. J. Microbiol. Methods 41 (2000) 85-112
27. Ootsubo M., Shimizu T., Tanaka R., Sawabe T., Tajima K., and Ezura Y. Seven-hour fluorescence in situ hybridization technique for enumeration of Enterobacteriaceae in food and environmental water sample. J. Appl. Microbiol. 95 (2003) 1182-1190
28. Piñeiro-Vidal M., Riaza A., and Santos Y. Tenacibaculum discolor sp. nov. and Tenacibaculum gallaicum sp. nov., isolated from sole (Solea senegalensis) and turbot (Psetta maxima) culture systems. Int. J. Syst. Evol. Microbiol. 58 (2008) 21-25
29. Poulsen L.K., Ballard G., and Stahl D.A. Use of rRNA fluorescence in situ hybridization for measuring the activity of single cells in young and established biofilms. Appl. Environ. Microbiol. 59 (1993) 1354-1360
30. Rice J., Sleigh M.A., Burkill P.H., Tarran G.A., O'Connor C.D., and Zubkov M.V. Flow cytometric analysis of characteristics of hybridization of species-specific fluorescent oligonucleotide probes to rRNA of marine nanoflagellates. Appl. Environ. Microbiol. 63 (1997) 938-944
31. Rodrigues U.M., and Kroll R.G. Rapid selective enumeration of bacteria in foods using a microcolony epifluorescence microscopy technique. J. Appl. Bacteriol. 64 (1988) 65-78
32. Savichtcheva O., Okayama N., Ito T., and Okabe S. Application of a direct fluorescence-based live/dead staining combined with fluorescence in situ hybridization for assessment of survival rate of Bacteroides spp. in drinking water. Biotechnol. Bioeng. 92 (2005) 356-363
33. Stackebrandt E., Kramer I., Swiderski J., and Hippe H. Phylogenetic basis for a taxonomic dissection of the genus Clostridium. FEMS Immunol. Med. Microbiol. 24 (1999) 253-258
34. Suzuki M., Nakagawa Y., Harayama S., and Yamamoto S. Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int. J. Syst. Evol. Microbiol. 51 (2001) 1639-1652
35. Wagner M., Schmid M., Juretschko S., Trebesius K.H., Bubert A., Goebel W., and Schleifer K.H. In situ detection of a virulence factor mRNA and 16S rRNA in Listeria monocytogenes. FEMS Microbiol. Lett. 160 (1998) 159-168
36. Wang R.F., Cao W.W., Franklin W., Campbell W., and Cerniglia C.E. A 16S rDNA-based PCR method for rapid and specific detection of Clostridium perfringens in food. Mol. Cell. Probes 8 (1994) 131-137
37. Wang X., Maegawa T., Karasawa T., Ozaki E., and Nakamura S. Clostridium sardiniense Prévot 1938 and Clostridium absonum Nakamura et al. 1973 are heterotypic synonyms: evidence from phylogenetic analyses of phospholipase C and 16S rRNA sequences, and DNA relatedness. Int. J. Syst. Evol. Microbiol. 55 (2005) 1193-1197
38. Wang X., Yamaguchi N., Someya T., and Nasu M. Rapid and automated enumeration of viable bacteria in compost using a micro-colony auto counting system. J. Microbiol. Methods 71 (2007) 1-6
39. Wise M.G., and Siragusa G.R. Quantitative detection of Clostridium perfringens in the broiler fowl gastrointestinal tract by real-time PCR. Appl. Environ. Microbiol. 71 (2005) 3911-3916
40. Yilmaz L.S., and Noguera D.R. Mechanistic approach to the problem of hybridization efficiency in fluorescent in situ hybridization. Appl. Environ. Microbiol. 70 (2004) 7126-7139