Phototoxicity of Ultraviolet (UV) radiation: Evaluation of UV-Blocking efficiency of intraocular lens (IOL) materials using retinal cell culture and in vitro bioassays

Open Toxicology Journal

Volumn 4, Issue , 2010, Pages 13-20

  Youn, H Y ^a   Cullen, A P ^a   Chou, B R ^a   Sivak, J G ^a  

^a UNIVERSITY OF WATERLOO   (Canada)

Author keywords

Alamar blue assay; Confocal microscopy; Hoechst assay; Intraocular lens; Phagocytotic activity assay; Retinal pigment epithelium; UV radiation

Indexed keywords

ALAMAR BLUE; DYE; HOE 33342; RHODAMINE 123; UNCLASSIFIED DRUG; BIOLOGICAL MARKER; DNA; FLUORESCENT DYE; INDICATOR; SILICONE;

ARTICLE; BIOASSAY; CELL DAMAGE; CELL STRUCTURE; CELL VIABILITY; CONTROLLED STUDY; DNA DAMAGE; DNA DEGRADATION; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IN VITRO STUDY; LENS IMPLANT; MATERIALS TESTING; MITOCHONDRIAL DNA DEPLETION; PHAGOCYTOSIS; PHOTOTOXICITY; PIGMENT EPITHELIUM; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RADIATION ABSORPTION; RADIATION DOSE; RADIATION EXPOSURE; RADIATION PROTECTION; RETINA CELL; SENSITIVITY ANALYSIS; THICKNESS; TOXICITY TESTING; ULTRAVIOLET B RADIATION; ULTRAVIOLET RADIATION; ALAMAR BLUE ASSAY; CELL CULTURE; CELL LINE; CELL NUCLEUS; CELL STRAIN ARPE 19; CONFOCAL MICROSCOPY; HOECHST ASSAY; MITOCHONDRION; PHAGOCYTOTIC ACTIVITY ASSAY; RADIATION ENERGY; RADIATION SHIELD; RHODAMINE 123 STAINING; SENSITIVITY AND SPECIFICITY;

EID: 77953393724     PISSN: None     EISSN: 18743404     Source Type: Journal    
DOI: 10.2174/1874340401004010013     Document Type: Article

References (59)

1. Boettner EA, Wolter JR. Transmission of the ocular media. Invest Ophthalmol Vis Sci 1962; 1: 776-83.
2. Ellerin BE, Nisce LZ, Roberts CW, et al. The effect of ionizing radiation on intraocular lenses. Int J Radiat Oncol Biol Phys 2001; 51: 184-208.
3. Mainster MA. Spectral transmittance of intraocular lenses and retinal damage from intense light sources. Am J Ophthalmol 1978; 85: 167-70.
4. rd. The nature of retinal radiation damage: dependence on wavelength, power level and exposure time. Vision Res 1980; 20: 1105-11.
5. Mainster MA, Ham WT Jr, Delori FC. Potential retinal hazards. Instrument and environmental light sources. Ophthalmology 1983; 90: 927-32.
6. Mainster MA. The spectra, classification, and rationale of ultraviolet-protective intraocular lenses. Am J Ophthalmol 1986; 102: 727-32.
7. Lin KK, Lin YC, Lee JS, Chao AN, Chen HSL. Spectral transmission characteristics of spectacle, contact, and intraocular lenses. Ann Ophthalmol 2002; 34: 206-15.
8. Mainster MA. Intraocular lenses should block UV radiation and violet but not blue light. Arch Ophthal 2005; 123: 550-5.
9. Mainster MA. Violet and blue light blocking intraocular lenses: photoprotection versus photoreception. Br J Ophthalmol 2006; 90: 784-92.
10. Thoms M, Fishman GA, Meulen DV. Spectral transmission characteristics of intraocular and aphakic contact lenses. Arch Ophthal 1983; 101: 92-3.
11. Alapetite C, Wachter T, Sage E, Moustacchi E. Use of the alkaline comet assay to detect DNA repair deficiencies in human fibroblasts exposed to UVC, UVB, UVA and gamma-rays. Int J Radiat Biol 1996; 69: 359-69.
12. Hiramitsu T, Armstrong D. Preventive effect of antioxidants on lipid peroxidation in the retina. Ophthalmic Res 1991; 23: 196-203.
13. Augustin AJ, Hunt S, Breipohl W, Böker T, Spitznas M. Influence of oxygen free radicals and free radical scavengers on the growth behaviour and oxidative tissue damage of bovine retinal pigment epithelium cells in vitro. Graefes Arch Clin Exp Ophthalmol 1996; 234: 58-63.
14. Kielbassa C, Roza L, Epe B. Wavelength dependence of oxidative DNA damage induced by UV and visible light. Carcinogenesis 1997; 18: 811-6.
15. Patton WP, Chakravarthy U, Davies RJ, Archer DB. Comet assay of UV-induced DNA damage in retinal pigment epithelial cells. Invest Ophthalmol Vis Sci 1999; 40: 3268-75.
16. Shimmura S, Tsubota K. Ultraviolet B-induced mitochondrial dysfunction is associated with decreased cell detachment of corneal epithelial cells in vitro. Invest Ophthalmol Vis Sci 1996; 38: 620-6.
17. Bantseev V, Youn HY. Mitochondrial movement and lens optics following oxidative stress from UV-B irradiation: cultured bovine lenses and human retinal pigment epithelial cells (ARPE-19) as examples. Ann N Y Acad Sci 2006; 1091: 17-33.
18. Youn HY, Bantseev V, Bols NC, Cullen AP, Sivak JG. In vitro assays for evaluating the ultraviolet B-induced damage in cultured human retinal pigment epithelial cells. J Photochem Photobiol B 2007; 88: 21-8.
19. Nielsen LK, Reid S, Greenfield PF. Cell cycle model to describe animal cell size variation and lag between cell number and biomass dynamics. Biotechnol Bioeng 2000; 56: 372-9.
20. American Conference of Governmental Industrial Hygienists (ACGIH). Threshold limit values for chemical substances and physical agents and biological exposure indices. Cincinnati, OH: USA 2006; pp. 164-70.
21. Oriowo OM, Cullen AP, Schirmer K, Chou BR, Bols NC, Sivak JG. Evaluation of a porcine lens and fluorescence assay approach for in vitro ocular toxicological investigations. Altern Lab Anim 2002; 30: 505-13.
22. O'Brien J, Wilson I, Orton T, Pognan F. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem 2000; 267: 5421-6.
23. Dayeh VR, Schirmer K, Lee LEJ, Bols NC. The use of fish-derived cell lines for investigation of environmental contaminants. Current protocols toxicology. Wiley: New York 2003; pp. 1-17.
24. Durand RE, Olive PL. Cytotoxicity, Mutagenicity and DNA damage by Hoechst 33342. J Histochem Cytochem 1982; 30: 111-6.
25. Darzynkiewicz Z, Traganos F, Staiano-Coico L, Kapuscinski J, Melamed MR. Interaction of rhodamine 123 with living cells studied by flow cytometry. Cancer Res 1982; 42: 799-806.
26. Young RW, Bok D. Participation of the retinal pigment epithelium in the rod outer segment renewal process. J Cell Biol 1969; 42: 392-403.
27. Abu-Amero KK, Bosley TM. Detection of mitochondrial respiratory dysfunction in circulating lymphocytes using resazurin. Arch Pathol Lab Med 2005; 129: 1295-8.
28. Zhang HX, Du GH, Zhang JT. Assay of mitochondrial functions by resazurin in vitro. Acta Pharmacol Sin 2004; 25: 385-9.
29. Steinert RF, Giamporcaro JE, Tasso VA. Clinical assessment of long-term safety and efficacy of a widely implanted silicone intraocular lens material. Am J Ophthalmol 1997; 123: 17-23.
30. Noell WK. Possible mechanisms of photoreceptor damage by light in mammalian eyes. Vision Res 1980; 20: 1163-71.
31. Gorgels TGMF, van Norren D. Ultraviolet and green light cause difference types of damage in rat retina. Invest Ophthalmol Vis Sci 1995; 36: 851-63.
32. Tratsk KS, Thanos S. UV irradiation causes multiple cellular changes in cultured human retinal pigment epithelium cells. Graefes Arch Clin Exp Ophthalmol 2003; 241: 852-9.
33. Bastianon C, Zanoni R, Miolo G, Caffieri S, Reddi E. Mitochondria and plasma membrane as targets of UVA-induced toxicity of neuroleptic drugs fluphenazine, perphenazine and thioridazine. Int J Biochem Cell Biol 2005; 37: 901-8.
34. Lu X, Wu RS. Ultraviolet damages sperm mitochondrial function and membrane integrity in the sea urchin Anthocidaris crassispina. Ecotoxicol Environ Saf 2005; 61: 53-9.
35. Paz ML, Maglio GDH, Weill FS, Bustamante J, Leoni J. Mitochondrial dysfunction and cellular stress progression after ultraviolet B irradiation in human keratinocytes. Photodermatol Photoimmunol Photomed 2008; 24: 115-22.
36. Oriowo OM. AlamarBlue bioassay for cellular investigation of UV-induced crystalline lens damage. Ophthalmic Physiol Opt 2003; 23: 307-14.
37. Hightower K, McCready J. Mechanisms involved in cataract development following near-ultraviolet radiation of cultured lenses. Curr Eye Res 1992; 11: 679-89.
38. Youn HY, Moran KL, Oriowo OM, Bols NC, Sivak JG. Surfactant and UV-B-induced damage of the cultured bovine lens. Toxicol In Vitro 2004; 18: 841-52.
39. Oriowo OM, Cullen AP, Sivak JG. Impairment of eye lens cell physiology and optics by broadband ultraviolet A-ultraviolet B radiation. Photochem Photobiol 2002; 76: 361-7.
40. Hosaka E, Soma Y, Kawa Y, et al. Effects of ultraviolet light on melanocyte differentiation: studies with mouse neural crest cells and neural crest-derived cell lines. Pig Cell Res 2004; 17: 150-7.
41. Reid L, Clothier RH, Khammo N. Hydrogen peroxide induced stress in human keratinocytes and its effect on bithionol toxicity. Toxicol In Vitro 2001; 15: 441-5.
42. Darzynkiewicz Z, Staiano-Coico L, Melamed MR. Increased mitochondrial uptake of rhodamine 123 during lymphocyte stimulation. Proc Natl Acad Sci USA 1981; 78: 2383-7.
43. Schneckenburger H, Gessler P, Pavenstädt-Grupp I. Measurements of mitochondrial deficiencies in living cells by microspectrofluorometry. J Histochem Cytochem 1992; 40: 1573-8.
44. Shimmura S, Tadano K, Tsubota K. UV dose-dependent caspase activation in a corneal epithelial cell line. Curr Eye Res 2004; 28: 85-92.
45. Shea CR, Wimberly J, Hasan T. Mitochondrial phototoxicity sensitized by doxycycline in cultured human carcinoma cells. J Invest Dermatol 1986; 87: 338-42.
46. Banerjee G, Gupta N, Kapoor A, Raman G. UV induced bystander signaling leading to apoptosis. Cancer Lett 2004; 223: 275-84.
47. Mocharla R, Mocharla H, Hodes ME. A novel, sensitive fluorometric staining technique for the detection of DNA in RNA preparations. Nucleic Acids Res 1987; 15: 10589.
48. Kressel M, Groscurth P. Distinction of apoptotic and necrotic cell death by in situ labeling of fragmented DNA. Cell Tissue Res 1994; 278: 549-56.
49. Ricci MS, Zong WX. Chemotherapeutic approaches for targeting cell death pathways. Oncologist 2006; 11: 342-57.
50. Worner W, Schrenk D. Influence of liver tumor promoters on apoptosis in rat hepatocytes induced by 2-acetylaminofluorene, ultraviolet light, or transforming growth factor beta 1. Cancer Res 1996; 56: 1272-8.
51. Baumstark-Khan C, Hentschel U, Nikandrova Y, Krug J, Horneck G. Fluorometric analysis of DNA unwinding (FADU) as a method for detecting repair-induced DNA strand breaks in UV-irradiated mammalian cells. Photochem Photobiol 2000; 72: 477-84.
52. Davis SK, Bardeen CJ. Cross-linking of histone proteins to DNA by UV illumination of chromatin stained with Hoechst 33342. Photochem Photobiol 2003; 77: 675-9.
53. Souvannavong V, Lemaire C, Brown S, Adam A. UV irradiation of a B-cell hybridoma increases expression of alkaline phosphatase: involvement in apoptosis. Biochem Cell Biol 1997; 75: 783-8.
54. La Vail MM. Rod outer segment disk shedding in rat retina: relationship to cyclic lighting. Science 1976; 194: 1071-4.
55. Young RW. The renewal of photoreceptor cell outer segments. J Cell Biol 1967; 33: 61-72.
56. Young RW. The daily rhythm of shedding and degradation of cone outer segment membranes in the lizard retina. J Ultrastruct Res 1977; 61: 172-85.
57. Sundelin S, Wihlmark U, Nilsson SE, Brunk UT. Lipofuscin accumulation in cultured retinal pigment epithelial cells reduces their phagocytic capacity. Curr Eye Res 1998; 17: 851-7.
58. Nilsson SE, Sundelin SP, Wihlmark U, Brunk UT. Aging of cultured retinal pigment epithelial cells: oxidative reactions, lipofuscin formation and blue light damage. Doc Ophthalmol 2003; 106: 13-6.
59. Seagle BL, Gasyna EM, Mieler WF, Norris JR Jr. Photoprotection of human retinal pigment epithelium cells against blue light- induced apoptosis by melanin free radicals from Sepia officinalis. Proc Natl Acad Sci USA 2006; 103: 16644-8.