In vitro assays for evaluating the ultraviolet B-induced damage in cultured human retinal pigment epithelial cells

Journal of Photochemistry and Photobiology B: Biology

Volumn 88, Issue 1, 2007, Pages 21-28

  Youn, Hyun Yi ^a   Bantseev, Vladimir ^a   Bols, Niels C ^a   Cullen, Anthony P ^a   Sivak, Jacob G ^a  

^a UNIVERSITY OF WATERLOO   (Canada)

Author keywords

Alamar blue assay; Cellular viability; Confocal microscopy; DNA damage; Mitochondrial damage; Phagocytotic activity; RPE; UVB induced retinal damage

Indexed keywords

ADULT; ARTICLE; CATARACT EXTRACTION; CELL CULTURE; CELL DAMAGE; CELL NUCLEUS; CELL STRUCTURE; CELL VIABILITY; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DNA DAMAGE; EYE PROTECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; LENS IMPLANT; MALE; MITOCHONDRION; PHAGOCYTOSIS; PIGMENT EPITHELIUM; PRIORITY JOURNAL; ULTRAVIOLET B RADIATION;

ACRIDINE ORANGE; CELL SURVIVAL; CELLS, CULTURED; DNA DAMAGE; EPITHELIAL CELLS; HUMANS; MICROSCOPY, CONFOCAL; OXAZINES; PHAGOCYTOSIS; PIGMENT EPITHELIUM OF EYE; ULTRAVIOLET RAYS; XANTHENES;

EID: 34447530462     PISSN: 10111344     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jphotobiol.2007.04.012     Document Type: Article

References (49)

1. Marmor M.F., and Wolfensberger T.J. The Retinal Pigment Epithelium: Function and Disease (1998), Oxford University Press, New York
2. Reme C., Reinboth J., Clausen M., and Hafezi F. Light damage revisited: converging evidence, diverging views?. Graefes Arch. Clin. Exp. Ophthalmol. 234 (1996) 2-11
3. McCarty C.A., and Taylor H.R. Recent developments in vision research: light damage in cataract. Invest. Ophthalmol. Vis. Sci. 37 9 (1996) 1720-1723
4. Kerr J.B., and McElroy C.T. Evidence for large upward trends of UVB radiation linked to ozone depletion. Science 262 (1993) 1032-1034
5. Noell W.K. Possible mechanisms of photoreceptor damage by light in mammalian eyes. Vision Res. 20 12 (1980) 1163-1171
6. Gorgels T.G.M.F., and van Norren D. Ultraviolet and green light cause difference types of damage in rat retina. Invest. Ophthalmol. Vis. Sci. 36 (1995) 851-863
7. Van Kuijk F.J.G.M. Effect of ultraviolet light on the eye: role of protective glasses. Environ. Health Perspet. 96 (1991) 177-195
8. Patton W.P., Charavarthy U., Davies R.J.H., and Archer D.B. Comet assay of UV-induced DNA damage in retinal pigment epithelial cells. Invest. Ophthalmol. Vis. Sci. 40 (1999) 3268-3275
9. Tratsk K.S., and Thanos S. UV irradiation causes multiple cellular changes in cultured human retinal pigment epithelium cells. Graefes Arch. Clin. Exp. Ophthalmol. 241 10 (2003) 852-859
10. Noell W.K., Walker V.S., Kang B.S., and Berman S. Retinal damage by light in rats. Invest. Ophthalmol. Vis. Sci. 5 (1966) 450-473
11. Beatty S., Koh H., Phil M., Henson D., and Boulton M. The role of oxidative stress in the pathogenesis of age-related macular degeneration. Surv. Ophthalmol. 45 2 (2000) 115-134
12. Norren D.V., and Vos J.J. Spectral transmission of the human ocular media. Vision Res. 14 11 (1974) 1237-1244
13. Mainster M.A. The spectra, classification, and rationale of ultraviolet-protective intraocular lenses. Am. J. Ophthalmol. 102 6 (1986) 727-732
14. Sparrow J.R., Nakanishi K., and Parish C.A. The lipofuscin fluorophore A2E mediates blue light-induced damage to retinal pigment epithelium. Invest. Ophthalmol. Vis. Sci. 41 7 (2000) 1981-1989
15. Godley B.F., Shamsi F.A., Liang F.Q., Jarrett S.G., Davies S., and Boulton M. Blue light induces mitochondrial DNA damage and free radical production in epithelial cells. J. Biol. Chem. 280 22 (2005) 21061-21066
16. American Conference of Governmental Industrial Hygienists (ACGIH), Threshold limit values for chemical substances and physical agents and biological exposure indices, Cincinnati, OH, USA, 2006, pp. 164-170.
17. Dayeh V.R., Schirme K., Lee L.E.J., and Bols N.C. The use of fish-derived cell lines for investigation of environmental contaminants. Curr. Protocols Toxicol. (2003), Wiley, New York pp. 1-17
18. Triel C., Vestergaard M.E., Bolund L., Jensen T.G., and Jensen U.B. Side population cells in human and mouse epidermis lack stem cell characteristics. Exp. cell Res. 295 1 (2004) 79-90
19. Lampidis T.J., Bernal S.D., Summerhayes I.C., and Chen L.B. Selective toxicity of rhodamine 123 in carcinoma cells in vitro. Cancer Res. 43 2 (1983) 716-720
20. Margrain T.H., Boulton M., Marshall J., and Sliney D.H. Do blue light filters confer protection against age-related macular degeneration?. Prog. Retin. Eye Res. 23 5 (2004) 523-531
21. Meyer-Rochow V.B. Risks, especially for the eye, emanating from the rise of solar UV-radiation in the Arctic and Antarctic regions. Int. J. Circumpolar Health 59 1 (2000) 38-51
22. Sadun A.C., Sadun A.A., and Sadun L.A. Solar retinopathy. A biophysical analysis. Arch. Ophthalmol. 102 10 (1984) 1510-1512
23. Young R.W. Solar radiation and age-related macular degeneration. Surv. Ophthalmol. 32 (1988) 252-269
24. Young R.W. Solar radiation and age-related macular degeneration. Surv. Ophthalmol. 56 3 (1988) 353-356
25. Hiramitsu T., and Armstrong D. Preventive effect of antioxidants on lipid peroxidation in the retina. Ophthal. Res. 23 4 (1991) 196-203
26. Wihlmark U., Wrigstad A., Roberg K., Brunk U.T., and Nilsson S.E. Formation of lipofuscin in cultured retinal pigment epithelial cells exposed to pre-oxidized photoreceptor outer segments. APMIS 104 4 (1996) 272-279
27. Augustin A.J., Hunt S., Breipohl W., Boker T., and Spitznas M. Influence of oxygen free radicals and free radical scavengers on the growth behaviour and oxidative tissue damage of bovine retinal pigment epithelium cells in vitro. Graefes Arch. Clin. Exp. Ophthalmol. 234 1 (1996) 58-63
28. Weiter J.J. Phototoxic changes in the retina. In: Miller D. (Ed). Clinical Light Damage to the Eye (1987), Springer, New York 79-125
29. Bynoe L.A., Gottsch J.D., Pou S., and Rosen G.M. Light-dependent generation of superoxide from human erythrocytes. Photochem. Photobiol. 56 3 (1992) 353-356
30. Kielbassa C., Roza L., and Epe B. Wavelength dependence of oxidative DNA damage induced by UV and visible light. Carcinogenesis 18 4 (1997) 811-816
31. Liang Y.G., Jorgensen A.G., Kaestel C.G., Wiencke A.K., Lui G.M., la Cour M.H., Ropke C.H., and Nissen M.H. Bcl-2, Bax, and c-Fos expression correlates to RPE cell apoptosis induced by UV-light and daunorubicin. Curr. Eye Res. 20 1 (2000) 25-34
32. Bok D. Processing and transport of retinoids by the retinal pigment epithelium. Eye 4 (1990) 326-332
33. Bastianon C., Zanoni R., Miolo G., Caffieri S., and Reddi E. Mitochondria and plasma membrane as targets of UVA-induced toxicity of neuroleptic drugs fluphenazine, perphenazine and thioridazine. Int. J. Biochem. Cell Biol. 37 4 (2005) 901-908
34. Lu X.Y., and Wu R.S. Ultraviolet damages sperm mitochondrial function and membrane integrity in the sea urchin Anthocidaris crassispina. Ecotoxicol. Environ. Saf. 61 1 (2005) 53-59
35. Bantseev V., and Youn H.Y. Mitochondrial "movement" and lens optics following oxidative stress from UV-B irradiation: cultured bovine lenses and human retinal pigment epithelial cells (ARPE-19) as examples. Ann. New York Acad. Sci. 1091 (2006) 17-33
36. Wang X., Sharma P.K., Gupta A., George V., Thomas A.J., Falcone T., and Agarwal A. Alterations in mitochondria membrane potential and oxidative stress in infertile men: a prospective observational study. Fertil. Steril. 80 (2003) 844-850
37. Sreekumar P.G., Kannan R., Yaung J., Spee C.K., Ryan S.J., and Hinton D.R. Protection from oxidative stress by methionine sulfoxide reductases in RPE cells. Biochem. Biophys. Res. Commun. 334 1 (2005) 245-253
38. Wong Y.T., Ruan R., and Tay F.E.H. Relationship between levels of oxidative DNA damage, lipid peroxidation and mitochondrial membrane potential in young and old F344 rats. Free Radic. Res. 40 4 (2006) 393-402
39. Graham J.K., Kunze E., and Hammerstedt R.H. Analysis of sperm cell viability, acrosomal integrity, and mitochondrial function using flow cytometry. Biol. Reprod. 43 1 (1990) 55-64
40. Green D.R., and Kroemer G. The pathophysiology of mitochondrial cell death. Science 305 5684 (2004) 626-629
41. Orrenius S. Mitochondrial regulation of apoptotic cell death. Toxicol. Lett. 149 1-3 (2004) 19-23
42. Sasai K., Yajima H., and Suzuki F. Suppression of postmitochondrial signaling and delayed response to UV-induced nuclear apoptosis in HeLa cells. Jpn. J. Cancer Res. 93 3 (2002) 275-283
43. Ershov A.V., Parkins N., Lukiw W.J., and Bazan N.G. Modulation of early response gene expression by prostagelandins in cultured rat retinal pigment epithelium cells. Curr. Eye Res. 6 (2000) 968-974
44. Godar D.E. Pre-programmed and programmed cell death mechanisms of apoptosis: UV-induced immediate and delayed apoptosis. Photochem. Photobiol. 63 (1996) 825-830
45. Strauss O., Stumpff F., Mergler S., Wienrich M., and Wiederholt M. The Royal College of Surgeons rat: an animal model for inherited retinal degeneration with a still unknown genetic defect. Acta Anat. (Basel) 162 2-3 (1998) 101-111
46. Kressel M., and Groscurth P. Distinction of apoptotic and necrotic cell death by in situ labeling of fragmented DNA. Cell Tissue Res. 278 (1994) 549-556
47. Au D.W.T. The application of histo-cytopathological biomarkers in marine pollution monitoring: a review. Mar. Pollut. Bull. 48 (2004) 817-834
48. Nielsen L.K., Reid S., and Greenfield P.F. Cell cycle model to describe animal cell size variation and lag between cell number and biomass dynamics. Biotechnol. Bioeng. 56 4 (2000) 372-379
49. Oriowo O.M., Cullen A.P., and Sivak J.G. Impairment of eye lens cellphysiology and optics by broadband ultraviolet A-ultraviolet B radiation. Photochem. Photobiol. 76 3 (2002) 361-367