Recombinant hemoglobin II from lucina pectinata: A large-scale method for hemeprotein expression in E. coli

Protein Journal

Volumn 29, Issue 2, 2010, Pages 143-151

  Ramos, Cacimar ^a   Pietri, Ruth ^a   Lorenzo, Wilmarie ^a   Roman, Elddie ^a   Granell, Laura B ^a   Cadilla, Carmen L ^b   López Garriga, Juan ^a  

^a UNIVERSITY OF PUERTO RICO   (Puerto Rico)

^b UNIVERSITY OF PUERTO RICO SCHOOL OF MEDICINE   (Puerto Rico)

Author keywords

Fermentor; Lucina pectinata; O2 binding; Recombinant hbii; Stopped flow kinetics

Indexed keywords

CHLORIDE; GLUCOSE; GLYCEROL; HEMIN; HEMOGLOBIN; HEMOPROTEIN; MAGNESIUM SULFATE; RECOMBINANT HEMOGLOBIN; RECOMBINANT HEMOGLOBIN II; UNCLASSIFIED DRUG; HYBRID PROTEIN; OXYGEN;

ARTICLE; BACTERIUM CULTURE; CELL CULTURE; CELL DENSITY; CLAM; ESCHERICHIA COLI; FERMENTATION; LUCINA PECTINATA; NONHUMAN; PROTEIN EXPRESSION; TEMPERATURE; VALIDATION PROCESS; ANIMAL; BINDING SITE; BIVALVE; CHEMICAL STRUCTURE; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; METHODOLOGY; PROTEIN CONFORMATION; PROTEIN ENGINEERING;

BIVALVIA; LUCINA PECTINATA;

ANIMALS; BINDING SITES; BIVALVIA; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; HEMEPROTEINS; HEMOGLOBINS; MODELS, MOLECULAR; OXYGEN; PROTEIN CONFORMATION; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS;

EID: 77953238444     PISSN: 15723887     EISSN: 15734943     Source Type: Journal    
DOI: 10.1007/s10930-010-9234-8     Document Type: Article

References (25)

1. Aristidou AA, San KY, Bennett GN (1995) Biotechnol Prog 11:475-478
2. Bradford MM (1979) Anal Biochem 72:248-254
3. Chow DC, Dreher MR, Trabbic-Carlson K, Chilkoti A (2006) Biotechnol Prog 22:638-646
4. Dantsker D, Roche C, Samuni U, Blouin G, Olson JS, Friedman JM (2005) J Biol Chem 280:38740-38755
5. De Jesús-Bonilla W, Jia Y, Alayash AI, López-Garriga J (2007) Biochemistry 46:10451-10460
6. Draghi F, Miele AE, Travaglini-Allocatelli C, Vallone B, Brunori M, Gibson QH, Olson JS (2002) J Biol Chem 277:7509-7519
7. Egawa T, Yeh S-R (2005) J Inor Biochem 99:72-96
8. Eiteman MA, Altman E (2006) Trends Biotechnol 24:530-536
9. Gavira JA, Camara-Artigas A, De Jesús-Bonilla W, López-Gar-riga J, Lewis A, Pietri R, Yeh S-R, Cadilla CL, García-Ruiz JM (2008) J Biol Chem 283:9414-9423
10. Graves PE, Henderson DP, Horstman MJ, Solomon BJ, Olson JS (2008) Biochim Biophys Acta 1784:471-479
11. Hoo H, Hashidoko Y, Islam MT, Tahara S (2004) Appl Environ Microbiol 70:5214-5221
12. Kraus DW, Wittenberg JB (1990) J Biol Chem 265:16043-16053
13. Kraus DW, Wittenberg JB (1990) J Biol Chem 265:16054-16059
14. León RG, Munier-Lehmann H, Barzu O, Baudin-Creuza V, Pietri R, López-Garriga J, Cadilla CL (2004) Protein Expr Purif 38:184-195
15. Liu T, Chen JY, Zheng Z, Wang TH, Chen GQ (2005) Appl Microbiol Biotechnol 68:346-354
16. Maillett DH, Simplaceanu V, Shen TJ, Ho NT, Olson JS, Ho C (2008) Biochemistry 47:10551-10563
17. Olson JS, Soman J, Phillips GN Jr (2007) IUBMB Life 59:552-562
18. Pietri R, León RG, Kiger L, Marden MC, Granell LB, Cadilla CL, López-Garriga J (2006) Biochim Biophys Acta 1764:758-765
19. Pietri R, Lewis A, León RG, Casabona G, Kiger L, Yeh SR, Fernandez-Alberti S, Marden MC, Cadilla CL, López-Garriga J (2009) Biochemistry 48:4881-4894
20. Pflug S, Richter SM, Urlacher VB (2007) J Biotechnol 129:481-488
21. Rivera L, López-Garriga J, Cadilla CL (2008) Gene 410:122-128
22. Rizzi M, Wittenberg JB, Coda A, Ascenzi P, Bolognesi M (1996) J Mol Biol 258:1-5
23. Springer BA, Egeberg KD, Sligar SG, Rohlfs RJ, Mathews AJ, Olson JS (1989) J Biol Chem 264:3057-3060
24. Studier FW (2005) Protein Expr Purif 41:207-234
25. Torres-Mercado E, Renta JY, Rodríguez Y, López-Garriga J, Cadilla CL (2003) J Protein Chem 22:683-690