High-level production of recombinant sulfide-reactive hemoglobin I from Lucina pectinata in Escherichia coli: High yields of fully functional holoprotein synthesis in the BLi5 E. coli strain

Protein Expression and Purification

Volumn 38, Issue 2, 2004, Pages 184-195

  León, Ruth Gretchen ^a,b   Munier Lehmann, Hélène ^c   Barzu, Octavian ^c   Baudin Creuza, Véronique ^d   Pietri, Ruth ^e   López Garriga, Juan ^e   Cadilla, Carmen L ^b  

^a UNIVERSITY OF PUERTO RICO RIO PIEDRAS   (Puerto Rico)

^b UNIVERSITY OF PUERTO RICO   (Puerto Rico)

^c INSTITUT PASTEUR   (France)

^d BICÊTRE HOSPITAL   (France)

^e UNIVERSITY OF PUERTO RICO   (Puerto Rico)

Author keywords

Expression; HbI; Hemoglobin; His tag; Purification

Indexed keywords

HEMOGLOBIN; HEMOGLOBIN I, NON MAMMALIAN; HEMOGLOBIN I, NON-MAMMALIAN; HYBRID PROTEIN; HYDROGEN SULFIDE;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BIOSYNTHESIS; BIVALVE; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; PROTEIN BINDING; SITE DIRECTED MUTAGENESIS; TEMPERATURE; ULTRAVIOLET SPECTROPHOTOMETRY;

AMINO ACID SEQUENCE; ANIMALS; BIVALVIA; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC VECTORS; HEMOGLOBINS; HYDROGEN SULFIDE; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROPHOTOMETRY, ULTRAVIOLET; TEMPERATURE;

BIVALVIA; ESCHERICHIA COLI; LUCINA; LUCINA PECTINATA; VECTORS; VERTEBRATA;

EID: 8844261238     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.08.014     Document Type: Article

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