Cloning and expression of a novel thermostable cellulase from newly isolated Bacillus subtilis strain I15

Molecular Biology Reports

Volumn 37, Issue 4, 2010, Pages 1923-1929

  Yang, Deliang ^a   Weng, Haibo ^a   Wang, Minge ^a   Xu, Weihua ^a   Li, Yaozhao ^a   Yang, Huiling ^b  

^a ZHENGZHOU UNIVERSITY   (China)

^b HENAN AGRICULTURAL UNIVERSITY   (China)

Author keywords

Bacillus subtilis I15; Clone and expression; Keywords Cellulase; Thermostable

Indexed keywords

CELLULASE; PROTEIN CELI15; RNA 16S; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL STRAIN; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; RNA SEQUENCE; THERMOSTABILITY; WILD TYPE; AMINO ACID SEQUENCE; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR GENETICS; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN TERTIARY STRUCTURE; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; TEMPERATURE;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

AMINO ACID SEQUENCE; BACILLUS SUBTILIS; CELLULASE; CLONING, MOLECULAR; CONSERVED SEQUENCE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, TERTIARY; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; TEMPERATURE;

EID: 77953141552     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-009-9635-y     Document Type: Article

References (23)

1. Beguin P, Aubert JP (1994) The biological degradation of cellulose. FEMS Microbiol Rev 13:25-58. doi:10.1111/j.1574-6976. 1994.tb00033.x
2. Ito S (1997) Alkaline cellulases from alkaliphilic Bacillus: enzymatic properties, genetics, and application to detergents. Extremophiles 1(2):61-66. doi:10.1007/s007920050015
3. Oksanen T, Poabilanina J (1998) Alkaline detergent enzymes from alkaliphile enzymtic properties, genetics and structures. Extremophiles 2(3):185-190. doi:10.1007/s007920050059 (Pubitemid 28407998)
4. Blanco A, Diaz P, Martinez J, Vidal T, Torres AL, Pastor F (1998) Cloning of a new endoglucanase gene from Bacillus sp. BP-23 characterization of enzyme, performance in paper manufacture from cereal straw. Appl Microbiol Biotechnol 50:48-54. doi:10.1007/s002530051255 (Pubitemid 28372375)
5. Doyle J, Pavel R, Barness G, Steinberger Y (2006) Cellulase dynamics in a desert soil. Soil Biol Biochem 38:371-376
6. Li YH, Ding M, Wang J, Xu GJ, Zhao F (2006) A novel thermoacidophilic endoglucanase, Ba-EGA, from a new cellulose- degrading bacterium, Bacillus sp. AC-1. Appl Microbiol Biotechnol 70:430-436. doi:10.1007/s00253-005-0075-x
7. Paula SP, Alexandra M, Jose CD, Maria R, Aires B, Maria CF (2002) Rapid production of thermostable cellulase free xylanase by a strain of Bacillus subtilis and its properties. Enzyme Microb Technol 30:924-933. doi:10.1016/S0141-0229(02)00034-0 (Pubitemid 34655416)
8. Li X, Gao P (1982) Isolation and partial properties of cellulosedecomposing strain of Cytophaga LX-7 from soil. Appl Environ Microbiol 38:148-158
9. Nazneen B, Javed M (2003) Characterization of a new Pseudomonas aeruginosa strain NJ-15 as a potential biocontrol agent. Curr Microbiol 46:324-328. doi:10.1007/s00284-002-3857-8 (Pubitemid 36919718)
10. Miller GL (1959) Use of dinitrosalicylic acid reagent for the determination of reducing sugar. Anal Chem 31:426-428. doi: 10.1021/ac60147a030
11. Kim CH, Kim DS (1992) Production and characterization of crystalline cellulose-degrading cellulase components from a thermophilic and moderately alkalophilic bacterium. J Microbiol Biotechnol 2:7-13
12. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual, 2nd edn. Cold SpringHarbor Laboratory Press, New York
13. Yoshihiro H, Kenzo K, Tadashi Y, Hajimi M, Tohru K, Susumu I (1997) Thermostable alkaline cellulase from an alkaliphilic isolate, Bacillus sp. KSM-S237. Extremophiles 1:151-156. doi: 10.1007/s007920050028
14. Robertson LD, Koehn RD (1978) Characteristics of the cellulase produced by the ascomycete Poronia punctata. Mycologia 70:1113-1121. doi:10.2307/3759142
15. Li W, Zhang WW, Yang MM, Chen YL (2008) Cloning of the thermostable cellulase gene from newly isolated Bacillus subtilis and its expression in Escherichia coli. Mol Biotechnol 40(2):195-201. doi:10.1007/s12033-008-9079-y
16. Thongekkaew J, Ikeda H, Masaki K, Iefuji H (2008) An acidic and thermostable carboxymethyl cellulase from the yeast Cryptococcus sp. S-2: purification, characterization and improvement of its recombinant enzyme production by high cell-density fermentation of Pichia pastoris. Protein Expr Purif 60(2):140-146. doi:10.1016/j.pep.2008.03.021
17. Aphichart K, Amorn P, Polkit S (2008) A novel thermostable endoglucanase from the wood-decaying fungus Daldinia eschscholzii (Ehrenb.:Fr.) Rehm. Enzym Microb Technol 42: 404-413. doi:10.1016/j.enzmictec.2007.11.009
18. Benjamaporn W, Kusol P, Katewadee B (2008) Cloning, expression and characterization of a thermotolerant endoglucanase from Syncephalastrum racemosum (BCC18080) in Pichia pastoris. Protein Expr Purif 58:78-86. doi:10.1016/j.pep.2007. 10.022
19. Aa K, Flengsrud R, Lindahl V, Tronsmo A (1994) Characterization of production and enzyme properties of an endo-beta-1, 4-glucanase from Bacillus subtilis CK-2 isolated from compost soil. Antonie Van Leeuwenhoek 66(4):319-326. doi:10.1007/ BF00882767
20. Arnold K, Bordoli L, Kopp J, Schwede T (2006) The SWISSMODEL workspace: A web-based environment for protein structure homology modeling. Bioinformatics 22:195-201. doi: 10.1093/bioinformatics/bti770 (Pubitemid 43205406)
21. Schwede T, Kopp J, Guex N, Peitsch MC (2003) SWISSMODEL: An automated protein homology-modeling server. Nucleic Acids Res 31:3381-3385. doi:10.1093/nar/gkg520 (Pubitemid 37442164)
22. Guex N, Peitsch MC (1997) SWISS-MODEL and the swiss-Pdb viewer: An environment for comparative protein modelling. Electrophoresis 18:2714-2723. doi:10.1002/elps.1150181505 (Pubitemid 28059943)
23. Sonan GK, Receveur-Brechot V, Duez C, Aghajari N, Czjzek M, Haser R, Gerday C (2007) The linker region plays a key role in the adaptation to cold of the cellulase from an Antarctic bacterium. Biochem J 407(2):293-302. doi:10.1042/BJ20070640 (Pubitemid 47556994)