A novel thermoacidophilic endoglucanase, Ba-EGA, from a new cellulose-degrading bacterium, Bacillus sp.AC-1

Applied Microbiology and Biotechnology

Volumn 70, Issue 4, 2006, Pages 430-436

  Li, Yan Hong ^a   Ding, Ming ^a   Wang, Ji ^a   Xu, Gen Jun ^a,b   Zhao, Fukun ^a,b  

^a INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

^b ZHEJIANG SCI TECH UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CARBON; CELLULOSE; DNA; ENZYME KINETICS; ION EXCHANGE; VISCOSITY;

AMMONIUM SULFATE FRACTIONATION; GEL FILTRATION CHROMATOGRAPHY; SODIUM DODECYL SULFATE (SDS);

BACTERIOLOGY;

AMMONIUM SULFATE; CELLULASE; CELLULOSE; DNA 16S; DODECYL SULFATE SODIUM; ENDOGLUCANASE BA EGA; GLUCAN SYNTHASE; POLYSORBATE 20; TRITON X 100; UNCLASSIFIED DRUG;

BACTERIUM; BIODEGRADATION; CELLULOSE;

AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS; BACTERIAL GROWTH; BACTERIUM CULTURE; BACTERIUM ISOLATION; CARBON SOURCE; CLOSTRIDIUM; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SUBSTRATE COMPLEX; FRACTIONATION; GEL FILTRATION CHROMATOGRAPHY; HYDROPHOBICITY; INCUBATION TIME; ION EXCHANGE; MICROBIAL DEGRADATION; MOLECULAR WEIGHT; MOLLUSC; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN DOMAIN; SEQUENCE HOMOLOGY; STOMACH JUICE; SUPERNATANT; THERMOSTABILITY; VISCOSITY;

AMINO ACID SEQUENCE; BACILLUS; CELLULASE; CELLULOSE; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; SUBSTRATE SPECIFICITY; TEMPERATURE;

AMPULLARIA; BACILLUS (BACTERIUM); BACTERIA (MICROORGANISMS); CLOSTRIDIUM; CROSSEA; MOLLUSCA;

EID: 33645220723     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-005-0075-x     Document Type: Article

References (30)

1. Bera-Maillet C, Arthaud L, Abad P, Rosso MN (2000) Biochemical characterization of MI-ENG1, a family 5 endoglucanase secreted by the root-knot nematode Meloidogyne incognita. Eur J Biochem 267:3255-3263
2. Bhat MK (2000) Cellulases and related enzymes in biotechnology. Biotechnol Adv 18:355-383
3. Bhat MK, Bhat S (1997) Cellulose degrading enzymes and their potential industrial applications. Biotechnol Adv 15:583-620
4. Bray MR, Johnson PE, Gilkes NR, McIntosh LP, Kilburn DG, Warren RA (1996) Probing the role of tryptophan residues in a cellulose-binding domain by chemical modification. Protein Sci 5:2311-2318
5. Carrard G, Linder M (1999) Widely different off rates of two closely related cellulose-binding domains from Trichoderma reesei. Eur J Biochem 262:637-643
6. Castanon M, Wilke CR (1981) Effects of the surfactant Tween 80 on enzymatic hydrolysis of newspaper. Biotechnol Bioeng 23:1365-1372
7. Chen SC, Qu YB, Zhang Y, Gao PJ (2000) Studies on submerged fermentation of alkaline beta-1,4-glycanases by Bacillus pumilus A-30. Sheng Wu Gong Cheng Xue Bao 16:485-489
8. Converse AO, Matsuno R, Tanaka M, Taniguchi M (1988) A model for enzyme adsorption and hydrolysis of microcrystalline cellulose with slow deactivation of the adsorbed enzyme. Biotechnol Bioeng 32:38-45
9. Creagh AL, Ong E, Jervis E, Kilburn DG, Haynes CA (1996) Binding of the cellulose-binding domain of exoglucanase Cex from Cellulomonas fimi to insoluble microcrystalline cellulose is entropically driven. Proc Natl Acad Sci U S A 93:12229-12234
10. Eckert K, Schneider E (2003) A thermoacidophilic endoglucanase (CeIB) from Alicyclobacillus acidocaldarius displays high sequence similarity to arabinofuranosidases belonging to family 51 of glycoside hydrolases. Eur J Biochem 270:3593-3602
11. Endo K, Hakamada Y, Takizawa S, Kubota H, Sumitomo N, Kobayashi T, Ito S (2001) A novel alkaline endoglucanase from an alkaliphilic Bacillus isolate: enzymatic properties, and nucleotide and deduced amino acid sequences. Appl Microbiol Biotechnol 57:109-116
12. Gilkes NR, Henrissat B, Kilburn DG, Miller RC Jr, Warren RA (1991) Domains in microbial beta-1,4-glycanases: sequence conservation, function, and enzyme families. Microbiol Rev 55:303-315
13. Hakamada Y, Hatada Y, Koike K, Yoshimatsu T, Kawai S, Kobayashi T, Ito S (2000) Deduced amino acid sequence and possible catalytic residues of a thermostable, alkaline cellulase from an Alkaliphilic bacillus strain. Biosci Biotechnol Biochem 64:2281-2289
14. Hakamada Y, Endo K, Takizawa S, Kobayashi T, Shirai T, Yamane T, Ito S (2002) Enzymatic properties, crystallization, and deduced amino acid sequence of an alkaline endoglucanase from Bacillus circulans. Biochim Biophys Acta 1570:174-180
15. Horikoshi K (1999) Alkaliphiles: some applications of their products for biotechnology. Microbiol Mol Biol Rev 63:735-750
16. Ishi A, Sheweita S, Doi RH (1998) Characterization of EngF from Clostridium cellulovorans and identification of a novel cellulose binding domain. Appl Environ Microbiol 64:1086-1090
17. Ito S, Kobayashi T, Ara K, Ozaki K, Kawai S, Hatada Y (1998) Alkaline detergent enzymes from alkaliphiles: enzymatic properties, genetics, and structures. Extremophiles 2:185-190
18. Kluepfel D (1988) Screening of prokaryotes for cellulose- and hemicellulose-degrading enzymes. Methods Enzymol 160:180-186
19. Lowry OH, Resebriugh NJ, Farr AL, Randall RJ (1959) Protein measurement with the folin phenol reagent. J Biol Chem 193:265-275
20. Mawadza C, Hatti-Kaul R, Zvauya R, Mattiasson B (2000) Purification and characterization of cellulases produced by two Bacillus strains. J Biotechnol 83:177-187
21. Moriyoshi K, Ohmoto T, Ohe T, Sakai K (2002) Purification and characterization of an endo-1,4-β-glucanase from Neisseria sicca SB that hydrolyzes β-1,4 linkages in cellulose acetate. Biosci Biotechnol Biochem 66:508-515
22. Nakashima K, Azuma J (2000) Distribution and properties of endobeta-1,4-glucanase from a lower termite, Coptotermes formosanus (Shiraki). Biosci Biotechnol Biochem 64:1500-1506
23. Ozawa T, Hakamada Y, Hatada Y, Kobayashi T, Shirai T, Ito S (2001) Thermostabilization by replacement of specific residues with lysine in a Bacillus alkaline cellulase: building a structural model and implications of newly formed double intrahelical salt bridges. Protein Eng 14:501-504
24. Shirai T, Ishida H, Noda J, Yamane T, Ozaki K, Hakamada Y, Ito S (2001) Crystal structure of alkaline cellulase K: insight into the alkaline adaptation of an industrial enzyme. J Mol Biol 310:1079-1087
25. Singh J, Batra N, Sobti RC (2004) Purification and characterisation of alkaline cellulase produced by a novel isolate, Bacillus sphaericus JS1. J Ind Microbiol Biotechnol 31:51-56
26. Sun Y, Cheng JY (2002) Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresour Technol 83:1-11
27. Suzuki KI, Ojima T, Nishita K (2003) Purification and cDNA cloning of a cellulase from abalone Haliotis discus hannai. Eur J Biochem 270:771-778
28. Tian X, Wang X (1998) Purification and properties of alkaline cellulase from alkalophilic Bacillus N6-27. Wei Sheng Wu Xue Bao 38:310-312
29. Wu J, Ju LK (1998) Enhancing enzymatic saccharification of waste newsprint by surfactant addition. Biotechnol Prog 14:649-652
30. Xu B, Hellman U, Ersson B, Janson JC (2000) Purification, characterization and amino-acid sequence analysis of a thermostable, low molecular mass endo-β-1,4-glucanase from blue mussel, Mytilus edulis. Eur J Biochem 267:4970-4977