Comparison of hybridization methods and real-time PCR: Their value in animal cell line characterization

Applied Microbiology and Biotechnology

Volumn 87, Issue 2, 2010, Pages 419-425

  Böhm Hofstätter, Helga ^a   Tschernutter, Marion ^a   Kunert, Renate ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

Gene copy number; mRNA; Northern blot; Real time PCR; Southern blot

Indexed keywords

ANIMAL CELL LINES; CELL CLONES; CELL LINES; CHARACTERIZATION STUDIES; CODING SEQUENCES; COPY NUMBER; EXPRESSION CONSTRUCTS; GENE COPY NUMBER; GENETIC PARAMETERS; GENETIC STABILITY; HYBRIDIZATION ANALYSIS; HYBRIDIZATION METHODS; MAMMALIAN CELL LINES; NORTHERN BLOT ANALYSIS; NORTHERN BLOTS; PCR METHOD; POLYMERASE CHAIN REACTION TECHNIQUES; PROTEIN PRODUCTION; REAL-TIME PCR; SOUTHERN BLOT; TRANSCRIPT LEVEL;

CELL CULTURE; CLONE CELLS; CLONING; MAMMALS; POLYMERASE CHAIN REACTION; SAFETY TESTING;

BIOINFORMATICS;

BIOTECHNOLOGY; GENETIC STRUCTURE; HYBRIDIZATION; MAMMAL; POLYMERASE CHAIN REACTION; PROTEIN; RNA;

CELL CLONE; CELL LINE; FLUORESCENCE IN SITU HYBRIDIZATION; GENE DOSAGE; GENE SEQUENCE; GENETIC CODE; GENETIC PARAMETERS; GENETIC STABILITY; GENETIC TRANSCRIPTION; INTERMETHOD COMPARISON; NONHUMAN; NORTHERN BLOTTING; REAL TIME POLYMERASE CHAIN REACTION; SHORT SURVEY; SOUTHERN BLOTTING; TRANSGENE; ANIMAL; CHEMISTRY; COMPARATIVE STUDY; GENE EXPRESSION; HUMAN; METABOLISM; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION; PROCEDURES;

ANIMALS; CELL LINE; GENE DOSAGE; GENE EXPRESSION; HUMANS; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION;

ANIMALIA; MAMMALIA;

EID: 77953082743     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2580-9     Document Type: Short Survey

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