Production of pig liver esterase in batch fermentation of E. coli Origami

Applied Microbiology and Biotechnology

Volumn 86, Issue 5, 2010, Pages 1337-1344

  Brüsehaber, Elke ^a   Schwiebs, Anja ^a   Schmidt, Marlen ^b   Böttcher, Dominique ^a   Bornscheuer, Uwe T ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

^b Enzymicals AG   (Germany)

Author keywords

Chaperones; E. coli Origami; Fermentation; Pig liver esterase

Indexed keywords

BATCH FERMENTATION; CELL DENSITY; CHAPERONES; CO-EXPRESSION; CULTIVATION CONDITIONS; CULTIVATION TIME; E. COLI; FED-BATCH FERMENTATION; FERMENTATION PROCESS; HIGH YIELD; MEDIA COMPOSITION; PIG LIVER ESTERASE; RECOMBINANT EXPRESSION; SHAKE FLASKS;

CULTIVATION; ENZYMES; ESCHERICHIA COLI; INDUSTRIAL APPLICATIONS; LIVER; PROCESS CONTROL;

FERMENTATION;

CHAPERONE; ESTERASE; ISOENZYME; PIG LIVER ESTERASE; UNCLASSIFIED DRUG;

COLIFORM BACTERIUM; ENZYME; EXPERIMENTAL STUDY; FERMENTATION; FUNCTIONAL MORPHOLOGY; GENE EXPRESSION; OPTIMIZATION; PIG; RECOMBINATION; TEMPERATURE EFFECT;

ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; CULTURE OPTIMIZATION; ENZYME ACTIVITY; ESCHERICHIA COLI; FED BATCH CULTURE; FED BATCH FERMENTATION; NONHUMAN; PROTEIN EXPRESSION;

AFFINITY LABELS; ANIMALS; CLONING, MOLECULAR; ESCHERICHIA COLI; ESTERASES; FERMENTATION; INDUSTRIAL MICROBIOLOGY; LIVER; RECOMBINANT FUSION PROTEINS; SWINE; TEMPERATURE; THIOREDOXIN-DISULFIDE REDUCTASE;

ESCHERICHIA COLI; SUIDAE;

EID: 77952889483     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2392-y     Document Type: Article

References (31)

1. Antranikian G (2006) Angewandte Mikrobiologie. Springer, Heidelberg
2. PH Bessette F Aslund J Beckwith G Georgiou 1999 Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm Proc Natl Acad Sci USA 96 13703 13708 10.1073/pnas.96.24.13703 1:CAS:528: DyaK1MXns1Oqsbk%3D
3. D Böttcher E Brüsehaber K Doderer UT Bornscheuer 2007 Functional expression of the gamma-isoenzyme of pig liver carboxyl esterase in Escherichia coli Appl Microbiol Biotechnol 73 1282 1289 10.1007/s00253-006-0585- 1 (Pubitemid 46089500)
4. Buchholz K, Kasche V, Bornscheuer UT (2005) Biocatalysts and enzyme technology. Wiley, Weinheim
5. JM Cutayar D Poillon 1989 High cell density culture of E. coli in a fed-batch system with dissolved oxygen as substrate feed indicator Biotechnol Lett 11 155 160 10.1007/BF01026048 1:CAS:528:DyaL1MXitFSqsbw%3D
6. A de Marco L Vigh S Diamant P Goloubinoff 2005 Native folding of aggregation-prone recombinant proteins in Escherichia coli by osmolytes, plasmid- or benzyl alcohol-overexpressed molecular chaperones Cell Stress Chaperones 10 329 339 10.1379/CSC-139R.1 (Pubitemid 41813380)
7. BR Glick 1995 Metabolic load and heterologous expression Biotechnol Adv 13 247 261 10.1016/0734-9750(95)00004-A 1:STN:280:DC%2BD3svnsVWmsw%3D%3D
8. FU Hartl M Hayer-Hartl 2002 Protein folding - Molecular chaperones in the cytosol: from nascent chain to folded protein Science 295 1852 1858 10.1126/science.1068408 1:CAS:528:DC%2BD38XhvFClsL4%3D (Pubitemid 34214115)
9. M Hermann MU Kietzmann M Ivancic C Zenzmaier RGM Luiten W Skranc M Wubbolts M Winkler R Birner-Gruenberger H Pichler H Schwab 2008 Alternative pig liver esterase (APLE) - Cloning, identification and functional expression in Pichia pastoris of a versatile new biocatalyst J Biotechnol 133 301 310 10.1016/j.jbiotec.2007.10.010 1:CAS:528:DC%2BD1cXhtlegsA%3D%3D (Pubitemid 50012393)
10. E Heymann W Junge 1979 Characterization of isoenzymes of pig-liver esterase 1. Chemical studies Eur J Biochem 95 509 518 10.1111/j.1432-1033.1979. tb12991.x 1:CAS:528:DyaE1MXktFGqsLo%3D (Pubitemid 9234039)
11. A Holmgren 1985 Thioredoxin Ann Rev Biochem 54 237 271 10.1146/annurev.bi.54.070185.001321 1:STN:280:DyaL2M3nsl2rug%3D%3D
12. A Hummel E Brüsehaber D Böttcher H Trauthwein K Doderer UT Bornscheuer 2007 Isoenzymes of pig liver esterase reveal striking differences in enantioselectivities Angew Chem Int Ed 46 8492 8494 10.1002/anie.200703256 1:CAS:528:DC%2BD2sXhtlOks7bP (Pubitemid 350114969)
13. R Kern A Malki A Holmgren G Richarme 2003 Chaperone properties of Escherichia coli thioredoxin and thioredoxin reductase Biochem J 371 965 972 10.1042/BJ20030093 1:CAS:528:DC%2BD3sXjtFKls7g%3D (Pubitemid 36578895)
14. Kietzmann M, Schwab H, Pichler H, Ivancic M, May O, Luiten RGM (2009) Preparation of an esterase. WIPO, Geneva, WO 2009/004093
15. N Krebsfänger F Zocher J Altenbuchner UT Bornscheuer 1998 Characterization, and enantioselectivity of a recombinant esterase from Pseudomonas fluorescens Enzyme Microb Technol 21 641 646 10.1016/S0141-0229(98) 00004-0 (Pubitemid 28260581)
16. ER LaVallie EA Diblasio S Kovacic KL Grant PF Schendel JM McCoy 1993 A thioredoxin gene fusion expression system that circumvents inclusion body formation in the Escherichia coli cytoplasm Nat Biotechnol 11 187 193 10.1038/nbt0293-187 1:CAS:528:DyaK3sXisFegsr0%3D (Pubitemid 23056045)
17. SY Lee 1996 High cell-density culture of Escherichia coli Trends Biotechnol 14 98 105 10.1016/0167-7799(96)80930-9 1:CAS:528:DyaK28XhsleqtLs%3D (Pubitemid 26079509)
18. D Liu RD Schmid M Rusnak 2006 Functional expression of Candida antarctica lipase B in the Escherichia coli cytoplasm-a screening system for a frequently used biocatalyst Appl Microbiol Biotechnol 72 1024 1032 10.1007/s00253-006-0369- 7 1:CAS:528:DC%2BD28XhtVWhsrjM (Pubitemid 44477381)
19. A Neubauer J Soini M Bollok M Zenker J Sandqvist J Myllyharju P Neubauer 2007 Fermentation process for tetrameric human collagen prolyl 4-hydroxylase in Escherichia coli: improvement by gene optimisation of the PDI/beta subunit and repeated addition of the inducer anhydrotetracycline J Biotechnol 128 308 321 10.1016/j.jbiotec.2006.10.017 1:CAS:528:DC%2BD2sXkvFGgsA%3D%3D (Pubitemid 46074202)
20. Novagen (2006) pET System Manual. EMD Biosciences, Merck KGaA, Darmstadt
21. WA Prinz F Aslund A Holmgren J Beckwith 1997 The role of the thioredoxin and glutaredoxin pathways in reducing protein disulfide bonds in Escherichia coli cytoplasm J Biol Chem 272 15661 15667 10.1074/jbc.272.25.15661 1:CAS:528:DyaK2sXktFCgtb4%3D (Pubitemid 27265536)
22. D Riesenberg 1991 High cell-density cultivation of Escherichia coli Curr Opin Biotechnol 2 380 384 10.1016/S0958-1669(05)80142-9 1:CAS:528: DyaK3MXmtlSnu70%3D
23. J Shiloach R Fass 2005 Growing E. coli to high cell density-a historical perspective on method development Biotechnol Adv 23 345 357 10.1016/j. biotechadv.2005.04.004 1:CAS:528:DC%2BD2MXks1Wntbg%3D (Pubitemid 40727987)
24. K Terpe 2003 Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems Appl Microbiol Biotechnol 60 523 533 1:CAS:528:DC%2BD3sXjvFGntQ%3D%3D
25. E Uchida N Kagawa T Sakaki N Urushino N Sawada M Kamakura M Ohta S Kato K Inouye 2004 Purification and characterization of mouse CYP27B1 overproduced by an Escherichia coli system coexpressing molecular chaperonins GroEL/ES Biochem Biophys Res Commun 323 505 511 10.1016/j.bbrc.2004.08.110 1:CAS:528: DC%2BD2cXnsVyqt78%3D (Pubitemid 39221126)
26. A Villaverde MM Carrio 2003 Protein aggregation in recombinant bacteria: biological role of inclusion bodies Biotechnol Lett 25 1385 1395 10.1023/A:1025024104862 1:CAS:528:DC%2BD3sXmtVems74%3D (Pubitemid 37065286)
27. AN Warrens MD Jones RI Lechler 1997 Splicing by overlap extension by PCR using asymmetric amplification: an improved technique for the generation of hybrid proteins of immunological interest Gene 186 29 35 10.1016/S0378-1119(96) 00674-9 1:CAS:528:DyaK2sXlslOjtQ%3D%3D (Pubitemid 27084826)
28. M Wittrup Larsen UT Bornscheuer K Hult 2008 Expression of Candida antarctica lipase B in Pichia pastoris and various Escherichia coli systems Protein Expr Purif 62 90 97 10.1016/j.pep.2008.07.012
29. YL Xu A Yasin R Tang JM Scharer M Moo-Young CP Chou 2008 Heterologous expression of lipase in Escherichia coli is limited by folding and disulfide bond formation Appl Microbiol Biotechnol 81 79 87 10.1007/s00253-008-1644-6 1:CAS:528:DC%2BD1cXht1OisbzJ
30. SS Yan J Yan G Shi Q Xu SC Chen YW Tian 2005 Production of native protein by using Synechocystis sp PCC6803 DnaB mini-intein in Escherichia coli Protein Expr Purif 40 340 345 10.1016/j.pep.2004.12.021 1:CAS:528:DC%2BD2MXit1Cjs70%3D (Pubitemid 40335917)
31. 1 by Escherichia coli using a computer-controlled cultivation process J Biotechnol 23 291 301 10.1016/0168-1656(92)90076-L 1:CAS:528:DyaK38XksVKls7g%3D