Methylglyoxal activates Gcn2 to phosphorylate eIF2α independently of the TOR pathway in Saccharomyces cerevisiae

Applied Microbiology and Biotechnology

Volumn 86, Issue 6, 2010, Pages 1887-1894

  Nomura, Wataru ^a   Maeta, Kazuhiro ^a,b   Kita, Keiko ^a   Izawa, Shingo ^a,c   Inoue, Yoshiharu ^a  

^a KYOTO UNIVERSITY   (Japan)

^b RIKEN   (Japan)

^c KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

EIF2 ; Gcn2; Methylglyoxal; Rapamycin; S. cerevisiae; TORC1

Indexed keywords

INITIATION FACTORS; KINASE ACTIVITY; METHYLGLYOXAL; POTENT INHIBITOR; PROTEIN SYNTHESIS; RAPAMYCIN; S.CEREVISIAE; SACCHAROMYCES CEREVISIAE; TARGET OF RAPAMYCIN;

PROTEINS; SYNTHESIS (CHEMICAL); YEAST;

PHOSPHORYLATION;

INITIATION FACTOR; METHYLGLYOXAL; PROTEIN EIF 2 ALPHA; PROTEIN GCN 2; PROTEIN KINASE; TARGET OF RAPAMYCIN KINASE; TRANSFER RNA; UNCLASSIFIED DRUG;

ALDEHYDE; DRUG; ENZYME ACTIVITY; GENETIC ANALYSIS; PROTEIN; YEAST;

AMINO TERMINAL SEQUENCE; ARTICLE; ENZYME ACTIVITY; NONHUMAN; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEIN SYNTHESIS; RNA BINDING; SACCHAROMYCES CEREVISIAE;

EUKARYOTIC INITIATION FACTOR-2; MODELS, BIOLOGICAL; PHOSPHORYLATION; PROTEIN BIOSYNTHESIS; PROTEIN-SERINE-THREONINE KINASES; PYRUVALDEHYDE; RNA, FUNGAL; RNA, TRANSFER; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SIROLIMUS; TRANSCRIPTION FACTORS;

SACCHAROMYCES CEREVISIAE;

EID: 77952876250     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2411-z     Document Type: Article

References (35)

1. T Beck MN Hall 1999 The TOR signalling pathway controls nuclear localization of nutrient-regulated transcription factors Nature 402 689 692 10.1038/45287 1:CAS:528:DC%2BD3cXjvVSh
2. PG Bertram JH Choi J Carvalho W Ai C Zeng TF Chan XF Zheng 2000 Tripartite regulation of Gln3p by TOR, Ure2p, and phosphatases J Biol Chem 275 35727 35733 10.1074/jbc.M004235200 1:CAS:528:DC%2BD3cXosVSksbg%3D
3. PG Bertram JH Choi J Carvalho TF Chan W Ai XF Zheng 2002 Convergence of TOR-nitrogen and Snf1-glucose signaling pathways onto Gln3 Mol Cell Biol 22 1246 1256 10.1128/MCB.22.4.1246-1252.2002 1:CAS:528:DC%2BD38XovVajsg%3D%3D (Pubitemid 34094859)
4. E Cameroni C De Virgilio O Deloche 2006 Phosphatidylinositol 4-phosphate is required for translation initiation in Saccharomyces cerevisiae J Biol Chem 281 38139 38149 10.1074/jbc.M601060200 1:CAS:528:DC%2BD28Xht12nu7nL (Pubitemid 46043305)
5. VA Cherkasova AG Hinnebusch 2003 Translational control by TOR and TAP42 through dephosphorylation of eIF2α kinase GCN2 Genes Dev 17 859 872 10.1101/gad.1069003 1:CAS:528:DC%2BD3sXislOltrw%3D (Pubitemid 36397374)
6. KH Cox JJ Tate TG Cooper 2002 Cytoplasmic compartmentation of Gln3 during nitrogen catabolite repression and the mechanism of its nuclear localization during carbon starvation in Saccharomyces cerevisiae J Biol Chem 277 37559 37566 10.1074/jbc.M204879200 1:CAS:528:DC%2BD38XnsVejsL0%3D
7. JL Crespo SB Helliwell C Wiederkehr P Demougin B Fowler M Primig MN Hall 2004 NPR1 kinase and RSP5-BUL1/2 ubiquitin ligase control GLN3-dependent transcription in Saccharomyces cerevisiae J Biol Chem 279 37512 37517 10.1074/jbc.M407372200 1:CAS:528:DC%2BD2cXntFSnsr4%3D (Pubitemid 39195463)
8. Dong J, Qiu H, Garcia-Barrio M, Anderson J, Hinnebusch AG (2000) Uncharged tRNA activates GCN2 by displacing the protein kinase moiety from a bipartite tRNA-binding domain. Mol Cell 6:269-279
9. M Garcia-Barrio J Dong S Ufano AG Hinnebusch 2000 Association of GCN1-GCN20 regulatory complex with the N-terminus of eIF2α kinase GCN2 is required for GCN2 activation EMBO J 19 1887 1899 10.1093/emboj/19.8.1887 1:CAS:528:DC%2BD3cXivVKltrk%3D
10. AG Hinnebusch 2005 Translational regulation of GCN4 and the general amino acid control of yeast Annu Rev Microbiol 59 407 450 10.1146/annurev.micro.59. 031805.133833 1:CAS:528:DC%2BD2MXht1ShsL7N (Pubitemid 41507438)
11. Y Inoue A Kimura 1995 Methylglyoxal and regulation of its metabolism in microorganisms Adv Microbiol Physiol 37 177 227 10.1016/S0065-2911(08)60146-0 1:CAS:528:DyaK28Xht1Cmu78%3D
12. Y Kamada T Funakoshi T Shintani K Nagano M Ohsumi Y Ohsumi 2000 Tor-mediated induction of autophagy via an Apg1 protein kinase complex J Cell Biol 150 1507 1513 10.1083/jcb.150.6.1507 1:CAS:528:DC%2BD3cXmslGlsr4%3D
13. H Kathleen KH Cox R Rai M Distler JR Daugherty JA Coffman TG Cooper 2000 Saccharomyces cerevisiae GATA sequences function as TATA elements during nitrogen catabolite repression and when Gln3p is excluded from the nucleus by overproduction of Ure2p J Biol Chem 275 17611 17618 10.1074/jbc.M001648200 (Pubitemid 30430807)
14. H Kubota K Ota Y Sakaki T Ito 2001 Budding yeast GCN1 binds the GI domain to activate the eIF2α kinase GCN2 J Biol Chem 276 17591 17596 10.1074/jbc.M011793200 1:CAS:528:DC%2BD3MXjvFGhsrc%3D
15. H Kubota T Obata K Ota T Sasaki T Ito 2003 Rapamycin-induced translational derepression of GCN4 mRNA involves a novel mechanism for activation of the eIF2α kinase GCN2 J Biol Chem 278 20457 20460 10.1074/jbc.C300133200 1:CAS:528:DC%2BD3sXkt1Wjtb0%3D (Pubitemid 36806341)
16. R Loewith E Jacinto S Wullschleger A Lorberg JL Crespo D Bonenfant W Oppliger P Jenoe MN Hall 2002 Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control Mol Cell 10 457 468 10.1016/S1097-2765(02)00636-6 1:CAS:528:DC%2BD38XnvFSlt74%3D (Pubitemid 35284167)
17. K Maeta S Izawa S Okazaki S Kuge Y Inoue 2004 Activity of the Yap1 transcription factor in Saccharomyces cerevisiae is modulated by methylglyoxal, a metabolite derived from glycolysis Mol Cell Biol 24 8753 8764 10.1128/MCB.24.19.8753-8764.2004 1:CAS:528:DC%2BD2cXnvVakur4%3D (Pubitemid 39245093)
18. K Maeta S Izawa Y Inoue 2005 Methylglyoxal, a metabolite derived from glycolysis, functions as a signal initiator of the high osmolarity glycerol-mitogen-activated protein kinase cascade and calcineurin/Crz1-mediated pathway in Saccharomyces cerevisiae J Biol Chem 280 253 280 1:CAS:528: DC%2BD2cXhtFGgu7rK
19. MJ Marton D Crouch AG Hinnebusch 1993 GCN1, a translational activator of GCN4 in Saccharomyces cerevisiae, is required for phosphorylation of eukaryotic translation initiation factor 2 by protein kinase GCN2 Mol Cell Biol 13 3541 3556 1:CAS:528:DyaK3sXlslOhtrY%3D
20. RR McCartney MC Schmidt 2001 Regulation of Snf1 kinase. Activation requires phosphorylation of threonine 210 by an upstream kinase as well as a distinct step mediated by the Snf4 subunit J Biol Chem 276 36460 36466 10.1074/jbc.M104418200 1:CAS:528:DC%2BD3MXnt1yrtrY%3D (Pubitemid 37384256)
21. T Noda Y Ohsumi 1998 Tor, a phosphatidylinositol kinase homologue, controls autophagy in yeast J Biol Chem 273 3963 3966 10.1074/jbc.273.7.3963 1:CAS:528:DyaK1cXht1ahsr8%3D (Pubitemid 28103257)
22. T Noda A Matsuura Y Wada Y Ohsumi 1995 Novel system for monitoring autophagy in the yeast Saccharomyces cerevisiae Biochem Biophys Res Commun 210 126 132 10.1006/bbrc.1995.1636 1:CAS:528:DyaK2MXlsF2mtr4%3D
23. W Nomura K Maeta K Kita S Izawa Y Inoue 2008 Role of Gcn4 for adaptation to methylglyoxal in Saccharomyces cerevisiae: Methylglyoxal attenuates protein synthesis through phosphorylation of eIF2α Biochem Biophys Res Commun 376 738 742 10.1016/j.bbrc.2008.09.063 1:CAS:528:DC%2BD1cXht1KlsrfN
24. H Qiu C Hu J Dong AG Hinnebusch 2002 Mutations that bypass tRNA binding activate the intrinsically defective kinase domain in GCN2 Genes Dev 16 1271 1280 10.1101/gad.979402 1:CAS:528:DC%2BD38XktFSmsb4%3D (Pubitemid 34553107)
25. N Sakumoto Y Mukai K Uchida T Kouchi J Kuwajima Y Nakagawa S Sugioka E Yamamoto T Furuyama H Mizubuchi N Ohsugi T Sakuno K Kikuchi I Matsuoka N Ogawa Y Kaneko S Harashima 1999 A series of protein phosphatase gene disruptants in Saccharomyces cerevisiae Yeast 15 1669 1679 10.1002/(SICI)1097-0061(199911)15: 15<1669::AID-YEA480>3.0.CO;2-6 1:CAS:528:DyaK1MXns1KjsLs%3D (Pubitemid 29537394)
26. S Sarkar AK Azad AK Hopper 1999 Nuclear tRNA aminoacylation and its role in nuclear export of endogenous tRNAs in Saccharomyces cerevisiae Proc Natl Acad Sci USA 96 14366 14371 10.1073/pnas.96.25.14366 1:CAS:528:DyaK1MXnvFKlurY%3D (Pubitemid 30000656)
27. A Schmidt T Beck A Koller J Kunz MN Hall 1998 The TOR nutrient signalling pathway phosphorylates NPR1 and inhibits turnover of the tryptophan permease EMBO J 17 6924 6931 10.1093/emboj/17.23.6924 1:CAS:528:DyaK1MXivFalsQ%3D%3D
28. R Stan MM McLaughlin R Cafferkey RK Johnson M Rosenberg GP Livi 1994 Interaction between FKBP12-rapamycin and TOR involves a conserved serine residue J Biol Chem 269 32027 32030 1:CAS:528:DyaK2cXmvFahtb0%3D
29. Y Takatsume S Izawa Y Inoue 2006 Methylglyoxal as a signal initiator for activation of the stress-activated protein kinase cascade in the fission yeast Schizosaccharomyces pombe J Biol Chem 281 9086 9092 10.1074/jbc.M511037200 1:CAS:528:DC%2BD28XjtVSktb0%3D (Pubitemid 43864621)
30. JJ Tate TG Cooper 2007 Stress-responsive Gln3 localization in Saccharomyces cerevisiae is separable from and can overwhelm nitrogen source regulation J Biol Chem 282 18467 18480 10.1074/jbc.M609550200 1:CAS:528:DC%2BD2sXmsVOks7k%3D (Pubitemid 47100261)
31. JJ Tate A Feller E Dubois TG Cooper 2006 Saccharomyces cerevisiae Sit4 phosphatase is active irrespective of the nitrogen source provided, and Gln3 phosphorylation levels become nitrogen source-responsive in a sit4-deleted strain J Biol Chem 281 37980 37992 10.1074/jbc.M606973200 1:CAS:528: DC%2BD28Xht1Kit7zP (Pubitemid 46042102)
32. CR Vazquez de Aldana MJ Marton AG Hinnebusch 1995 GCN20, a novel ATP binding cassette protein, and GCN1 reside in a complex that mediates activation of the eIF-2α kinase GCN2 in amino acid-starved cells EMBO J 14 3184 3199 1:CAS:528:DyaK2MXntVekurg%3D
33. SA Wek S Zhu RC Wek 1995 The histidyl-tRNA synthetase-related sequence in the eIF-2α protein kinase GCN2 interacts with tRNA and is required for activation in response to starvation for different amino acids Mol Cell Biol 15 4497 4506 1:CAS:528:DyaK2MXntVGiur0%3D
34. S Wullschleger R Loewith MN Hall 2006 TOR signaling in growth and metabolism Cell 124 471 484 10.1016/j.cell.2006.01.016 1:CAS:528: DC%2BD28Xhslaqs74%3D (Pubitemid 43199434)
35. R Yang SA Wek RC Wek 2000 Glucose limitation induces GCN4 translation by activation of Gcn2 protein kinase Mol Cell Biol 20 2706 2717 10.1128/MCB.20.8.2706-2717.2000 1:CAS:528:DC%2BD3cXit1ymu78%3D (Pubitemid 30183489)