NanoSIMS imaging of Bacillus spores sectioned by focused ion beam

Journal of Microscopy

Volumn 238, Issue 3, 2010, Pages 189-199

  Weber, P K ^a   Graham, G A ^a   Teslich, N E ^a   Chan, W Moberly ^a   Ghosal, S ^a   Leighton, T J ^b   Wheeler, K E ^b  

^a LAWRENCE LIVERMORE NATIONAL LABORATORY   (United States)

^b CHILDREN S HOSPITAL OAKLAND RESEARCH INSTITUTE   (United States)

Author keywords

FIB; Microbial forensics; NanoSIMS; Sample preparation

Indexed keywords

BACTERIOLOGY; ELECTRONS; FOCUSED ION BEAMS; FORENSIC SCIENCE; HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY; SCANNING ELECTRON MICROSCOPY; SECONDARY EMISSION; SECONDARY ION MASS SPECTROMETRY;

BACTERIAL SPORE; FIB; FOCUSED IONS BEAMS; HIGH RESOLUTION; ION IMAGES; LIFT OUTS; MICROBIAL FORENSIC; NANOSIMS; SAMPLE PREPARATION; SECONDARY ION-MASS SPECTROMETRY;

IONS;

ARTICLE; BACTERIAL SPORE; CELL STRUCTURE; CONTROLLED STUDY; ELECTRON BEAM; INTERMETHOD COMPARISON; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; SCANNING TRANSMISSION ELECTRON MICROSCOPY; STAINING; BACILLUS; CHEMISTRY; IMAGE PROCESSING; PROCEDURES; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

BACILLUS (BACTERIUM); BACTERIA (MICROORGANISMS);

BACILLUS; IMAGE PROCESSING, COMPUTER-ASSISTED; MICROSCOPY, ELECTRON, TRANSMISSION; SPECTROMETRY, MASS, SECONDARY ION; SPORES;

EID: 77952625232     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2009.03336.x     Document Type: Article

References (37)

1. Badro, J., Ryerson, F., Weber, P.K., Ricolleau, A., Fallon, S.J., Hutcheon, I.D. Albarède, F. (2007) Chemical imaging with NanoSIMS: a window into deep-Earth geochemical processes. Earth Planet. Sci. Lett. 262, 543 551.
2. Boxer, S.G., Kraft, M.L. Weber, P.K. (2009) Advances in imaging secondary ion mass spectrometry for biological samples. Ann. Rev. Biophys. 38, 53 74.
3. Bradley, J., Dai, Z.R., Erni, R. et al 2005) An astronomical 2175 Å feature in interplanetary dust particles. Science 307, 244 247. (Pubitemid 40116236)
4. Brewer, L.N., Ohlhausen, J.A., Kotula, P.G. Michael, J.R. (2008) Forensic analysis of bioagents by X-ray and TOF-SIMS hyperspectral imaging. Forensic Sci. Int. 179, 98 106.
5. Chandra, S. Morrison, G.H. (1992) Sample preparation of animal tissues and cell cultures for secondary ion mass spectrometry (SIMS) microscopy. Biol. Cell 74, 31 42.
6. Cliff, J.B., Jarman, K.H., Valentine, N.B., Golledge, S.L., Gaspar, D.J., Wunschel, D.S. Wahl, K.L. (2005) Differentiation of spores of Bacillus subtilis grown on different media by elemental characterization using time-of-flight secondary ion mass spectrometry. Appl. Environ. Microbiol. 71, 6524 6530.
7. De Veirman, A. Weaver, L. (1999) The use of a focused-ion-beam machine to prepare transmission electron microscopy samples of residual photoresist. Micron 30, 213 220.
8. Drobne, D., Milani, M., Zrimec, A., Leser, V. Berden Zrimec, M. (2005) Electron and ion imaging of gland cells using the FIB/SEM system. J. Microsc. 219, 29 35.
9. Floss, C., Stadermann, F.J., Bradley, J., Dai, Z.R., Bajt, S. Graham, G. (2004) Carbon and nitrogen isotopic anomalies in an anhydrous interplanetary dust particle. Science 303, 1355 1358.
10. Gerhardt, P., Pankratz, H.S. Scherrer, R. (1976) Fine structure of the Bacillus thuringiensis spore. Appl. Environ. Microbiol. 32, 438 440.
11. Ghosal, S., Fallon, S.J., Leighton, T., Wheeler, K.E., Hutcheon, I.D. Weber, P.K. (2008) Imaging and 3D elemental characterization of intact bacterial spores with high-resolution secondary ion mass spectrometry (NanoSIMS) depth profile analysis. Anal. Chem. 80, 5986 5992.
12. Ghosal, S., Leighton, T., Wheeler, K., Hutcheon, I.D. Weber, P.K. (in review) Spatially resolved characterization of water and ion incorporation in Bacillus spores.
13. Giannuzzi, L.A., Kempshall, B.W., Schwarz, S.M., Lomness, J.K., Prenitzer, B.I. Stevie, F.A. (2005) FIB lift-out specimen preparation techniques. Introduction to Focused lon Beams-Instrumentation, Theory, Techniques and Practice (ed. by L.A. Giannuzzi and F.A. Stevie pp. 201 228. Springer
14. Gikunju, C.M., Lev, S.M., Birenzvige, A. Schaefer, D.M. (2004) Detection and identification of bacteria using direct injection inductively coupled plasma mass spectrometry. Talanta 62, 741 744.
15. Graham, G.A., Teslich, N.E., Kearsley, A.T. et al 2008) Applied focused ion beam techniques to the sample preparation of astromaterials for integrated nanoanalysis. Meteorit. Planet. Sci. 43, 561 569.
16. He, L.M. Tebo, B.M. (1998) Surface charge properties of and Cu(II) adsorption by spores of the marine Bacillus sp. Strain SG-1. Appl. Environ. Microbiol. 64, 1123 1129.
17. Hillion, F., Daigne, B., Girard, F. Slodzian, G. (1993) A new high performance instrument: the CAMECA NanoSIMS 50. Secondary Ion Mass Spectrometry: SIMS IX (ed. by A. Benninghoven, Y. Nihei, R. Shimizu & H.W. Werner pp. 254 257. John Wiley & Sons
18. Horita, J. Vass, A.A. (2003) Stable-isotope fingerprints of biological agents as forensic tools. J. Forensics Sci. 48, 1 5.
19. Kamino, T., Yaguchi, T., Hashimoto, T., Ohnishi, T. & Umemura, K. (2005) A FIB micro-sampling techniques and a site specific TEM specimen preparation method. Introduction to Focused Ion Beams-Instrumentation, Theory, Techniques and Practice (ed. by L.A. Giannuzzi and F.A. Stevie pp. 229 246. Springer
20. Kotula, P.G., Keenan, M.R. Michael, J.R. (2006) Tomographic spectral imaging with multivariate statistical analysis: comprehensive 3D microanalysis. Microsc. Microanal. 12, 36 48.
21. Kreuzer-Martin, H.W. Jarman, K.H. (2007) Stable isotope ratios and forensic analysis of microorganisms. Appl. Environ. Microbiol. 73, 3896 3908.
22. Kreuzer-Martin, H.W., Lott, M.J., Dorigan, J.V. Ehleringer, J.R. (2003) Microbe forensics: oxygen and hydrogen stable isotope ratios in Bacillus subtilis cells and spores. Proc. Natl. Acad. Sci. USA 100, 815 819.
23. Kreuzer-Martin, H.W., Chesson, L.A., Lott, M.J., Dorigan, J.V. Ehleringer, J.R. (2004) Stable isotope ratios as a tool in microbial forensics-part1: microbial isotopic composition as a function of growth medium. J. Forensics Sci. 49, 1 7.
24. Longchamp, P. Leighton, T. (2000) Molecular recognition specificity of Bacillus globigii spore antibodies. Lett. Appl. Microbiol. 31, 242 246.
25. Moreau, J.W., Weber, P.K., Martin, M.C., Gilbert, B., Hutcheon, I.D. Banfield, J.F. (2007) Extracellular proteins limit the dispersal of biogenic nanoparticles. Science 316, 1600 1603.
26. Nakanura-Messenger, K., Messenger, S., Keller, L.P., Clemett, S.J. Zolensky, M.E. (2006) Organic globules in the Tagish Lake meteorite remnants of the protosolar disk. Science 314, 1439 1442.
27. Niewöhner, L. Wenz, H.W. (1999) Applications of focused ion beam systems in gunshot residue investigation. J. Forensic Sci. 44, 105 109.
28. Ohne, D.F. Murrel, W.G. (1973) Exosporium and spore coat formation in Bacillus cereus T. J. Bacteriol. 115, 1179 1190.
29. Reyntjens, S. Kübel, C. (2005) Scanning/transmission electron microscopy and dual-beam sample preparation for analysis of crystalline materials. J. Cryst. Growth 275, 1849 1856.
30. Rode, L.J. Foster, J.W. (1962) Ionic germination of spores of Bacillus megaterium QM B1551. Arch. Mikrobiol. 43, 183 200.
31. Ryzhov, V., Hathout, Y. Fenselau, C. (2000) Rapid characterization of spores of Bacillus cereus group bacteria by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry. Appl. Environ. Microbiol. 66, 3828 3834.
32. Stewart, M., Somlyo, A.P., Somlyo, A.V., Suman, H., Lindsay, J.A. Murrell, W.G. (1980) Distribution of calcium and other elements in cryosectioned Bacillus cereus T spores, determined by high-resolution scanning electron probe X-ray microanalysis. J. Bacteriol. 143, 481 491.
33. Stewart, M., Somlyo, A.P., Somlyo, A., Suman, H., Lindsay, J.A. Murrell, W.G. (1981) Scanning electron probe X-ray microanalysis of elemental distributions in freeze-dried cryosections of Bacillus coagulans spores. J. Bacteriol. 147, 670 674.
34. 3 grains from asymptotic giant branch stars. Science 305, 1455 1457.
35. Westphal, A.J., Price, P.B., Leighton, T.J. Wheeler, K.E. (2003) Kinetics of size changes of individual Bacillus thuringiensis spores in response to changes in relative humidity. Proc. Natl. Acad. Sci. USA 100, 3461 3466.
36. Whiteaker, J.R., Fenselau, C., Fetterolf, D., Steele, D. Wilson, D. (2004) Quantitative determination of heme for forensic characterization of Bacillus spores using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal. Chem. 76, 2836 2841.
37. Wilson, R.G., Stevie, F.A. Magee, C.W. (1989) Secondary Ion Mass Spectrometry: A Practical Handbook for Depth Profiling and Bulk Impurity Analysis. Wiley