Microtubule-mediated transport of the tumor-suppressor protein Merlin and its mutants

Proceedings of the National Academy of Sciences of the United States of America

Volumn 107, Issue 16, 2010, Pages 7311-7316

  Benseñor, Lorena B ^a   Barlan, Kari ^a   Rice, Sarah E ^a   Fehon, Richard G ^b   Gelfand, Vladimir I ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

^b UNIVERSITY OF CHICAGO   (United States)

Author keywords

Dynein; Intracellular transport; Kinesin; Microtubules; Neurofibromatosis

Indexed keywords

DYNEIN ADENOSINE TRIPHOSPHATASE; KINESIN 1; MERLIN; MUTANT PROTEIN; TUMOR SUPPRESSOR PROTEIN; WT1 PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; CELL CULTURE; CELLULAR DISTRIBUTION; CYTOSKELETON; DROSOPHILA; GENE EXPRESSION; GENE MUTATION; IMAGE ANALYSIS; INTRACELLULAR TRANSPORT; MICROTUBULE; NEUROFIBROMATOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; PROTEIN TRANSPORT;

ANIMALS; BIOLOGICAL TRANSPORT; CONCANAVALIN A; CYTOPLASM; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; DYNEINS; ENDOCYTOSIS; GREEN FLUORESCENT PROTEINS; KINESIN; MICROTUBULES; MODELS, BIOLOGICAL; NEUROFIBROMIN 2; NUCLEAR PROTEINS; PHOSPHORYLATION; PROTEIN STRUCTURE, TERTIARY; TRANS-ACTIVATORS;

EID: 77952163960     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0907389107     Document Type: Article

References (39)

1. Rouleau GA, et al. (1993) Alteration in a new gene encoding a putative membrane-organizing protein causes neurofibromatosis type 2. Nature 363:515-521.
2. Hara T, Bianchi AB, Seizinger BR, Kley N (1994) Molecular cloning and characterization of alternatively spliced transcripts of the mouse neurofibromatosis 2 gene. Cancer Res 54:330-335. (Pubitemid 24053161)
3. Bianchi AB, et al. (1994) Mutations in transcript isoforms of the neurofibromatosis 2 gene in multiple human tumour types. Nat Genet 6:185-192.
4. McClatchey AI (2003) Merlin and ERM proteins: Unappreciated roles in cancer development? Nat Rev Cancer 3:877-883.
5. Gutmann DH, et al. (1999) Increased expression of the NF2 tumor-suppressor gene product, merlin, impairs cell motility, adhesion and spreading. Hum Mol Genet 8:267-275.
6. Lallemand D, et al. (2009) Merlin regulates transmembrane receptor accumulation and signaling at the plasma membrane in primary mouse Schwann cells and in human schwannomas. Oncogene 28:854-865.
7. McClatchey AI, Giovannini M (2005) Membrane organization and tumorigenesis - the NF2 tumor suppressor, Merlin. Genes Dev 19:2265-2277. (Pubitemid 41396902)
8. Brault E, et al. (2001) Normal membrane localization and actin association of the NF2 tumor suppressor protein are dependent on folding of its N-terminal domain. J Cell Sci 114:1901-1912. (Pubitemid 32530045)
9. Cole BK, Curto M, Chan AW, McClatchey AI (2008) Localization to the cortical cytoskeleton is necessary for Nf2/merlin-dependent epidermal growth factor receptor silencing. Mol Cell Biol 28:1274-1284. (Pubitemid 351214130)
10. James MF, Manchanda N, Gonzalez-Agosti C, Hartwig JH, Ramesh V (2001) The neurofibromatosis 2 protein product merlin selectively binds F-actin but not G-actin, and stabilizes the filaments through a lateral association. Biochem J 356:377-386. (Pubitemid 32532348)
11. Stickney JT, Bacon WC, Rojas M, Ratner N, Ip W (2004) Activation of the tumor suppressor merlin modulates its interaction with lipid rafts. Cancer Res 64:2717-2724. (Pubitemid 38500607)
12. McCartney BM, Fehon RG (1996) Distinct cellular and subcellular patterns of expression imply distinct functions for the Drosophila homologues of moesin and the neurofibromatosis 2 tumor suppressor, merlin. J Cell Biol 133:843-852.
13. LaJeunesse DR, McCartney BM, Fehon RG (1998) Structural analysis of Drosophila merlin reveals functional domains important for growth control and subcellular localization. J Cell Biol 141:1589-1599.
14. Stokowski RP, Cox DR (2000) Functional analysis of the neurofibromatosis type 2 protein by means of disease-causing point mutations. Am J Hum Genet 66:873-891.
15. Gutmann DH, Hirbe AC, Haipek CA (2001) Functional analysis of neurofibromatosis 2 (NF2) missense mutations. Hum Mol Genet 10:1519-1529. (Pubitemid 32684894)
16. Giovannini M, et al. (1999) Schwann cell hyperplasia and tumors in transgenic mice expressing a naturally occurring mutant NF2 protein. Genes Dev 13:978-986. (Pubitemid 29200981)
17. Johnson KC, Kissil JL, Fry JL, Jacks T (2002) Cellular transformation by a FERM domain mutant of the Nf2 tumor suppressor gene. Oncogene 21:5990-5997.
18. Rong R, Surace EI, Haipek CA, Gutmann DH, Ye K (2004) Serine 518 phosphorylation modulates merlin intramolecular association and binding to critical effectors important for NF2 growth suppression. Oncogene 23:8447-8454. (Pubitemid 39540795)
19. Kissil JL, Johnson KC, Eckman MS, Jacks T (2002) Merlin phosphorylation by p21-activated kinase 2 and effects of phosphorylation on merlin localization. J Biol Chem 277:10394-10399. (Pubitemid 34968159)
20. Hughes SC, Fehon RG (2006) Phosphorylation and activity of the tumor suppressor Merlin and the ERM protein Moesin are coordinately regulated by the Slik kinase. J Cell Biol 175:305-313.
21. Badouel C, et al. (2009) The FERM-domain protein Expanded regulates Hippo pathway activity via direct interactions with the transcriptional activator Yorkie. Dev Cell 16:411-420.
22. Hamaratoglu F, et al. (2006) The tumour-suppressor genes NF2/Merlin and Expanded act through Hippo signalling to regulate cell proliferation and apoptosis. Nat Cell Biol 8:27-36. (Pubitemid 43064794)
23. McCartney BM, Kulikauskas RM, LaJeunesse DR, Fehon RG (2000) The neurofibromatosis-2 homologue, Merlin, and the tumor suppressor expanded function together in Drosophila to regulate cell proliferation and differentiation. Development 127:1315-1324.
24. Fraenzer JT, et al. (2003) Overexpression of the NF2 gene inhibits schwannoma cell proliferation through promoting PDGFR degradation. Int J Oncol 23:1493-1500.
25. McClatchey AI, Fehon RG (2009) Merlin and the ERM proteins: regulators of receptor distribution and signaling at the cell cortex. Trends Cell Biol 19:198-206.
26. Maitra S, Kulikauskas RM, Gavilan H, Fehon RG (2006) The tumor suppressors Merlin and Expanded function cooperatively to modulate receptor endocytosis and signaling. Curr Biol 16:702-709.
27. Curto M, Cole BK, Lallemand D, Liu CH, McClatchey AI (2007) Contact-dependent inhibition of EGFR signaling by Nf2/Merlin. J Cell Biol 177:893-903. (Pubitemid 46873094)
28. Ling SC, Fahrner PS, Greenough WT, Gelfand VI (2004) Transport of Drosophila fragile X mental retardation protein-containing ribonucleoprotein granules by kinesin-1 and cytoplasmic dynein. Proc Natl Acad Sci USA 101:17428-17433.
29. Rothbauer U, et al. (2008) A versatile nanotrap for biochemical and functional studies with fluorescent fusion proteins. Mol Cell Proteomics 7:282-289. (Pubitemid 351298442)
30. Pilling AD, Horiuchi D, Lively CM, Saxton WM (2006) Kinesin-1 and Dynein are the primary motors for fast transport of mitochondria in Drosophila motor axons. Mol Biol Cell 17:2057-2068. (Pubitemid 44011618)
31. Hakimi MA, Speicher DW, Shiekhattar R (2002) The motor protein kinesin-1 links neurofibromin and merlin in a common cellular pathway of neurofibromatosis. J Biol Chem 277:36909-36912.
32. Schroer TA (2004) Dynactin. Annu Rev Cell Dev Biol 20:759-779.
33. Gauger AK, Goldstein LSB (1993) The Drosophila kinesin light chain: Primary structure and interaction with kinesin heavy chain. J Biol Chem 268:13657-13666. (Pubitemid 23307798)
34. Li W, et al. (2010) Merlin/NF2 suppresses tumorigenesis by inhibiting the E3 ubiquitin ligase CRL4(DCAF1) in the nucleus. Cell 140:477-490.
35. Koga H, et al. (1998) Impairment of cell adhesion by expression of the mutant neurofibromatosis type 2 (NF2) genes which lack exons in the ERM-homology domain. Oncogene 17:801-810. (Pubitemid 28430202)
36. Dorogova NV, et al. (2008) The role of Drosophila Merlin in spermatogenesis. BMC Cell Biol 9:1-15.
37. Grönholm M, et al. (1999) Homotypic and heterotypic interaction of the neurofibromatosis 2 tumor suppressor proteinmerlin and the ERMprotein ezrin. J Cell Sci 112:895-904. (Pubitemid 29179322)
38. Gautreau A, et al. (2003) Isolation and characterization of an aggresome determinant in the NF2 tumor suppressor. J Biol Chem 278:6235-6242. (Pubitemid 36800879)
39. Golovnina K, Blinov A, Akhmametyeva EM, Omelyanchuk LV, Chang LS (2005) Evolution and origin of merlin, the product of the Neurofibromatosis type 2 (NF2) tumorsuppressor gene. BMC Evol Biol 5:69-86. (Pubitemid 41805067)