Increased expression of the NF2 tumor suppressor gene product, merlin, impairs cell motility, adhesion and spreading

Human Molecular Genetics

Volumn 8, Issue 2, 1999, Pages 267-275

  Gutmann, David H ^a   Sherman, Larry ^b   Seftor, Liz ^c   Haipek, Carrie ^a   Lu, Kimberly Hoang ^a   Hendrix, Mary ^c  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

^c UNIVERSITY OF IOWA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EZRIN; GENE PRODUCT; MERLIN; MOESIN; RADIXIN;

ANIMAL CELL; ARTICLE; CELL ADHESION; CELL GROWTH; CELL LINE; CELL MOTILITY; CELL SPREADING; CONTROLLED STUDY; GENE EXPRESSION; GENE MUTATION; GENE OVEREXPRESSION; NEURILEMOMA; NEUROFIBROMATOSIS; NONHUMAN; PRIORITY JOURNAL; RAT; SEQUENCE HOMOLOGY; TUMOR SUPPRESSOR GENE;

ANIMALIA; FALCO COLUMBARIUS;

EID: 0032899713     PISSN: 09646906     EISSN: None     Source Type: Journal    
DOI: 10.1093/hmg/8.2.267     Document Type: Article

References (33)

1. Parry, D.M. and Eldridge, R. (1991) Bilateral Acoustic Neurofibromatosis (NF2): Consensus Development Conference. National Institutes of Health, Bethesda, MD.
2. Evans, D.G.R., Huson, S.M., Donnai, D., Neary, W., Blair, V., Newton, V. and Harris, R. (1992) A clinical study of type 2 neurofibromatosis. Q. J. Med., 304, 603-618.
3. Rouleau, G.A., Merel, P., Lutchman, M., Sanson, M., Zucman, J., Marineau, C., Hoang-Xuan, K., Demczuk, M., Desmaze, C., Plougastel, B., Pulst, S.M., Lenoir, G., Bijisma, E., Fashold, R., Dumanski, J., de Jong, P., Parry, D., Eldrige, R., Aurias, A., Delattre, O. and Thomas, G. (1993) Alteration in a new gene encoding a putative membrane-organizing protein causes neurofibromatosis type 2. Nature, 363, 515-521.
4. Trofatter, J.A., MacCollin, M.M., Rutter, J.L., Murrell, J.R., Duyao, M.P., Parry, D.M., Eldridge, R., Kley, N., Menon, A.G., Pulaski, K., Haase, V.H., Ambrose, C.M., Munroe, D., Bove, C., Haines, J.L., Martuza, R.L., MacDonald, M.E., Seizinger, B.R., Short, M.P., Buckler, A.J. and Gusella, J.F. (1993) A novel moesin-, ezrin-, radixin-like gene is a candidate for the neurofibromatosis 2 tumor suppressor. Cell, 72, 1-20.
5. Gutmann, D.H. (1997) Molecular insights into neurofibromatosis 2. Neurobiol. Dis., 3, 247-261.
6. Tsukita, S., Yonemura, S. and Tsukita, S. (1997) ERM (ezrin/radixin/moesin) family: from cytoskeleton to signal transduction. Curr. Opin. Cell Biol., 9, 70-75.
7. Lutchman, M. and Rouleau, G.A. (1995) The neurofibromatosis type 2 gene product, schwannomin, suppresses growth of NIH 3T3 cells. Cancer Res., 55, 2270-2274.
8. Sherman, L., Xu, H.-M., Geist, R.T., Saporito-Irwin, S., Howells, N., Ponta, H., Herrlich, P. and Gutmann, D.H. (1997) Interdomain binding mediates tumor growth suppression by the NF2 gene product. Oncogene, 15, 2505-2509.
9. Xu, H.-M. and Gutmann, D.H. (1998) Merlin differentially associates with the microtubule and actin cytoskeleton. J. Neurosci. Res., 51, 403-415.
10. den Bakker, M.A., Riegman, P.H.J., Hekman, R.A.C.P., Coersma, W., Janssen, P.J.A., van der Kwast, T.H. and Zwarthoff, E.C. (1995) The product of the NF2 tumour suppressor gene localizes near the plasma membrane and is highly expressed in muscle cells. Oncogene, 10, 757-763.
11. denBakker, M.A., Tacilar, M., Reigman, P.H.J., Hekman, A.C.P., Boersma, W., Janssen, P.J.A., deJong, T.A.W., Hendriks, Q., van der Kwast, T.H. and Zwartoff, E. (1995) Neurofibromatosis type 2 protein co-localizes with elements of the cytoskeleton. Am. J. Pathol., 147, 1339-1349.
12. Sainio, M., Zhao, F., Heiska, L., Turunen, O., den Bakker, M., Zwarthoff, E., Lutchman, M., Rouleau, G.A., Jaaskelainen, J., Vaheri, A. and Carpen, O. (1997) Neurofibromatosis 2 tumor suppressor protein co-localizes with ezrin and CD44 and associates with actin-containing cytoskeleton. J. Cell Sci., 110, 2249-2260.
13. Deguen, B., Merel, P., Goutebroze, L., Giovannini, M., Reggio, H., Arpin, M. and Thomas, G. (1998) Impaired interaction of naturally occurring mutant NF2 protein with actin-based cytoskeleton and membrane. Hum. Mol. Genet., 7, 217-226.
14. Gonzalez-Agosti, C., Xu, L., Pinney, D., Beauchamp, R., Hobbs, W., Gusella, J. and Ramesh, V. (1996) The merlin tumor suppressor localizes preferentially in membrane ruffles. Oncogene, 13, 1239-1247.
15. Scherer, S.S. and Gutmann, D.H. (1996) Expression of the neurofibromatosis 2 tumor suppressor gene product, merlin, in Schwann cells. J. Neurosci. Res., 46, 595-605.
16. Pelton, P.D., Sherman, L., Rizvi, T., Marchionni, M., Wood, P., Friedman, R. and Ratner, N. (1998) Ruffling membrane, stress fiber, cell spreading and proliferation abnormalities in human schwannoma cells. Oncogene, 17, 2195-2209.
17. Gutmann, D.H., Geist, R.T., Xu, H.-M., Kim, J.S. and Saporito-Irwin, S. (1998) Defects in neurofibromatosis 2 protein function can arise at multiple levels. Hum. Mol. Genet., 7, 335-345.
18. Hendrix, M.J.C., Seftor, E.A., Seftor, R.E.B. and Fidler, I.J. (1987) A simple quantitative assay for studying the invasive potential of high and low metastatic varients. Cancer Lett., 38, 137-147.
19. Chu, Y.-W., Runyan, R.B., Oshima, R.G. and Hendrix, M.J.C. (1993) Expression of complete keratin filaments in mouse L cells augments cell migration and invasion. Proc. Natl Acad. Sci. USA, 90, 4261-4265.
20. Peden, K.W.C., Charles, C., Sanders, L. and Tennekoon, G.I. (1989) Isolation of rat Schwann cell lines: use of SV40 T antigen gene regulated by synthetic metallothionein promoters. Exp. Cell Res., 185, 60-72.
21. Rubinfeld, B., Souza, B., Albert I., Muller, O., Chamberlain, S.H., Masiarz, F.R., Munemitsu, S. and Polakis, P. (1993) Association of the APC gene product with beta-catenin. Science, 262, 1731-1734.
22. Huynh, D.P. and Pulst, S.-M. (1996) Neurofibromatosis 2 antisense oligodeoxynucleotides induce reversible inhibition of schwannomin synthesis and cell adhesion in STS26T and T98G cells. Oncogene, 13, 73-84.
23. McClatchey, A.I., Saotome, I., Mercer, K., Crowley, D., Gusella, J.F., Bronson, R.T. and Jacks, T. (1998) Mice heterozygous for a mutation at the Nf2 tumor suppressor locus develop a range of highly metastatic tumors. Genes Dev., 12, 1121-1133.
24. Shaw, R.J., McClatchey, A.I. and Jacks, T. (1998) Regulation of the neurofibromatosis type 2 tumor suppressor protein, merlin, by adhesion and growth arrest stimulation. J. Biol. Chem., 279, 7757-7764.
25. Shaw, R.J., Henry, M., Solomon, F. and Jacks, T. (1998) RhoA-dependent phosphorylation and relocalization of ERM proteins into apical membrane/actin protrusions in fibroblasts. Mol. Biol. Cell, 9, 403-419.
26. Scoles, D.R., Huynh, D.P., Morcos, P.A., Coulsell, E.R., Robinson, N.G.G., Tamanoi, F. and Pulst, S.-M. (1998) Neurofibromatosis 2 tumour suppressor schwannomin interacts with beta-II-spectrin. Nature Genet., 18, 354-359.
27. Sato, N., Yonemura, S., Obinata, T., Tsukita, S. and Tsukita, S. (1992) A gene family consisting of ezrin, radixin, and moesin. Its specific localization at actin filament/plasma membrane association sites. J. Cell Biol., 103, 131-143.
28. Berryman, M., Franck, Z. and Bretscher, A. (1993) Ezrin is concentrated in the apical microvilli of a wide variety of epithelial cells, whereas moesin is found primarily in endothelial cells. J. Cell Sci., 105, 1025-1043.
29. Franck, Z., Gary, R. and Bretscher, A. (1993) Moesin, like ezrin, co-localizes with actin in the cortical cytoskeleton in cultured cells, but its expression is more variable. J. Cell Sci., 105, 219-231.
30. Mackay, D.J.G., Esch, F., Furthmayr, H. and Hall, A. (1997) Rho- and Rac-dependent assembly of focal adhesion complexes and actin filaments in permeabilized fibroblasts: an essential role for ezrin/radixin/moesin proteins. J. Cell Biol., 138, 927-938.
31. Hirao, M., Sato, N., Kondo, T., Yonemura, S., Monden, M., Sasaki, T., Takai, Y., Tsukita, S. and Tsukita, S. (1996) Regulation mechanism of ERM (ezrin/radixin/moesin) protein/plasma membrane association: possible involvement of phosphatidylinositol turnover and Rho-dependent signaling pathway. J. Cell Biol., 135, 37-51.
32. 1/S control of anchorage-independent growth in the fibroblast cell cycle. J. Cell Biol., 115, 1419-1425.
33. Gutmann, D.H., Giordano, M.J., Fishback, A.S. and Guha, A. (1997) Loss of merlin expression in sporadic meningiomas, ependymomas and schwannomas. Neurology, 48, 267-270.