Structural analysis of Drosophila Merlin reveals functional domains important for growth control and subcellular localization

Journal of Cell Biology

Volumn 141, Issue 7, 1998, Pages 1589-1599

  LaJeunesse, Dennis R ^a   McCartney, Brooke M ^a   Fehon, Richard G ^a,b  

^a DUKE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b DUKE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; DNA; EZRIN; GENE PRODUCT; MOESIN; PROTEIN; RADIXIN;

ALLELE; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DROSOPHILA; GENE MUTATION; GROWTH REGULATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN STRUCTURE; TUMOR SUPPRESSOR GENE;

ANIMALS; BINDING SITES; CELL LINE; DROSOPHILA; FEMALE; GENES, INSECT; MALE; MEMBRANE PROTEINS; MUTAGENESIS; NEUROFIBROMIN 2; RECOMBINANT FUSION PROTEINS; SUBCELLULAR FRACTIONS;

FALCO COLUMBARIUS;

EID: 0032578033     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.141.7.1589     Document Type: Article

References (40)

1. Algrain, M., O. Turunen, A. Vaheri, D. Louvard, and M. Arpin. 1993. Ezrin contains cytoskeleton and membrane binding domains accounting for its proposed role as a membrane-cytoskeletal linker. J. Cell Biol. 120:129-139.
2. Berryman, M., R. Gary, and A. Bretscher. 1995. Ezrin oligomers are major cytoskeletal components of placental microvilli: a proposal for their involvement in cortical morphogenesis. J. Cell Biol. 131:1231-1242.
3. Boedigheimer, M., and A. Laughon. 1993. expanded: a gene involved in the control of cell proliferation in imaginal discs. Development (Camb.). 118: 1291-1301.
4. Brand, A.H., and N. Perrimon. 1993. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development (Camb.). 118:401-415.
5. Bretscher, A., R. Gary, and M. Berryman. 1995. Soluble ezrin purified from placenta exists as stable monomers and elongated dimers with masked COOH-terminal czrin-radixin-moesin association domains. Biochemistry. 34:16830-16837.
6. Brown, M.A. 1997. Tumor suppressor genes and human cancer. Adv. Genet. 36: 45-135.
7. Bryant, P.J., K.L. Watson, R.W. Justice, and D.F. Woods. 1993. Tumor suppressor genes encoding proteins required for cell interactions and signal transduction in Drosophila. Dev. Suppl. 239-249.
8. Edgar, B.A., and C.F. Lehner. 1996. Developmental control of cell cycle regulators - a fly's perspective. Science. 274:1646-1652.
9. Fehon, R.G., P.J. Kooh, I. Rebay, C.L. Regan, T. Xu, M.A.T. Muskavitch, and S. Artavanis-Tsakonas. 1990. Molecular interactions between the protein products of the neurogenic loci Notch and Delta, two EGF-homologous genes in Drosophila. Cell. 61:523-534.
10. Fehon, R.G., I.A. Dawson, and S. Artavanis-Tsakonas. 1994. A Drosophila homologue of membrane-skeleton protein 4.1 is associated with septate junctions and is encoded by the coracle gene. Development (Camb.). 120:545-557.
11. Fehon, R.G., T. Oren, D.R. LaJeunesse, T.E. Melby, and B.M. McCartney. 1997. Isolation of mutations in the Drosophila homologues of the human Neurofibromatosis 2 and yeast CDC42 genes using a simple and efficient reverse-genetic method. Genetics. 146:245-252.
12. Franck, Z., R. Gary, and A. Bretscher. 1993. Moesin, like ezrin, colocalizes with actin in the cortical cyloskeleton in cultured cells, but its expression is more variable. J. Cell Sci. 105:219-231.
13. Gary, R., and A. Bretscher. 1995. Ezrin self-association involves binding of an N-terminal domain to a normally masked C-terminal domain that includes the F-actin binding site. Mol. Biol. Cell. 6:1061-1075.
14. Golic, K.G., and S. Lindquist. 1989, The FLP recombinase of yeast catalyses site-specific recombination in the Drosophila genome. Cell. 59:499-509.
15. Gonzalez-Agosti, C., L. Xu, D. Pinney, R. Beauchamp, W. Hobbs, J. Gusella, and V. Ramesh. 1996. The merlin tumor suppressor localizes preferentially in membrane ruffles. Oncogene. 13:1239-1247.
16. Haase, V.H., J.A. Trofatter, M. MacCollin, E. Tarttelin, J.F. Gusella, and V. Ramesh. 1994. The murine NF2 homologue encodes a highly conserved merlin protein with alternative forms. Hum. Mol. Genet. 3:407-411.
17. Helander, T., O. Carpen, O. Turunen, P.E. Kovanen, A. Vaheri, and T. Timonen. 1996. ICAM-2 redistributed by ezrin as a target for killer cells. Miture. 382:265-268.
18. Hirao, M., N. Sato, T. Kondo, S. Yonemura, M. Monden, T. Sasaki, Y. Takai, S. Tsukita, and S. Tsukita. 1996. Regulation mechanism of ERM (ezrin/radixin/ moesin) protein/plasma membrane association: possible involvement of phosphatidylinositol turnover and Rho-dependent signaling pathway. J. Cell Biol. 135:37-51.
19. Jacoby, L.B., M. MacCollin, R. Barone, V. Ramesh, and J.F. Gusella. 1996. Frequency and distribution of NF2 mutations in schwannomas. Genes Chromosomes Cancer. 17:45-55.
20. Mackay, D.J., F. Esch, H. Furthmayr, and A. Hall. 1997. Rho-and rac-dependent assembly of focal adhesion complexes and actin filaments in permeabilized fibroblasts: an essential role for ezrin/radixin/moesin proteins. J. Cell Biol. 138:927-938.
21. Mahoney, P.A., U. Weber, P. Onofrechuk, H. Biessmann, P.J. Bryant, and C.S. Goodman. 1991. The fat tumor suppressor gene in Drosophila encodes a novel member ol the cadherin gene superfamily. Cell. 67:853-868.
22. Matsui, T., M. Maeda, Y. Doi, S. Yonemura, M. Amano, K. Kaibuchi, and S. Tsukita. 1998. Rho-kinase phosphorylates COOH-terminal threonines of ezrin/radixin/moesin (ERM) proteins and regulates their head-to-tail association. J. Cell Biol. 140:647-657.
23. McCartney, B.M., and R.G. Fehon. 1996. Distinct cellular and subcellular patterns of expression imply distinct functions lor the Drosophila homologues of moesin and the neurofibromatosis 2 tumor suppressor, merlin. J. Cell Biol. 133:843-852.
24. McCartney, B.M., and R.G. Fehon. 1997. The ERM family of proteins and their roles in cell-cell interactions. In Cytoskeletal-Membrane Interactions and Signal Transduction. P. Cowin and M.W. Klymkowsky, editors. R.G. Landes Hioscicnce. Austin, TX. 200-210.
25. McClatchey, A.I., I. Saotome, V. Ramesh, J.F. Gusella, and T. Jacks. 1997. The Nf2 tumor suppressor gone product is essential for extraembryonic development immediately prior to gastrulation. Genes Dev. 11:1253-1265.
26. Pykett, M.J., M. Murphy, P.R. Harnish, and D.L. George. 1994. The neurofibromatosis 2 (NF2) tumor suppressor gene encodes multiple alternatively spliced transcripts. Hum. Mol. Genet. 3:559-564.
27. Rebay, I., R.G. Fehon, and S. Artavanis-Tsakonas. 1993. Specific truncations of Drosophila Notch define dominant activated and dominant negative forms of the receptor. Cell. 74:319-329.
28. Rouleau, G.A., P. Merel, M. Lutchman, M. Sanson, J. Zucman, C. Marineau, K. Hoang-Suan. S. Demezuk, C. Desmaze, B. Plougastel, et al. 1993. Alteration in a new gene encoding a putative membrane-organizing protein causes neurofibromatosis type 2. Nature. 363:515-521.
29. Sainio, M., F. Zhao, L. Heiska, O. Turunen, M. den Bakker, E. Zwarthoff, M. Lutchman, M.A. Rouleau, J. Jaaskelainen, A. Vaheri, and O. Carpen. 1997. Neurofibromatosis 2 tumor suppressor protein colocalizes with ezrin and CD44 and associates with actin-containing cytoskeleton. J. Cell Sci. 110: 2249-2260.
30. Sherman, L., H.M. Xu, R.T. Geist, S. Saporito-Irwin, N. Howells, H. Ponta, P. Herrlich, and D.H. Gutmann. 1997. Interdomain binding mediates tumor growth suppression by the NF2 gene product. Oncogene. 15:2505-2509.
31. Takaishi, K., T. Sasaki, T. Kameyama, S. Tsukita, S. Tsukita, and Y. Takai. 1995. Translocation of activated Rho from the cytoplasm to membrane ruffling area, cell-cell adhesion sites and cleavage furrows. Oncogene. 11:39-48.
32. Takeuchi, K., N. Sato, H. Kasahara, N. Funayama, A. Nagafuchi, S. Yonemura, S. Tsukita, and S. Tsukita. 1994. Perturbation of cell adhesion and microvilli formation by antisense oligonucleotides to ERM family members. J. Cell Biol. 125:1371-1384.
33. Tomlinson, A., and D.F. Ready. 1987. Cell fate in the Drosophila ommatidium. Dev. Biol. 123:264-275.
34. Trofatter, J.A., M.M. MacCollin, J.L. Rutter, R. Eldridge, N. Kiev, A.G. Menon, K. Pulaski, H. Haase, C.M. Ambrose, D. Munroe, et al. 1993. A novel moesin-, ezrin-, radixin-like gene is a candidate for the neurofibromatosis 2 tumor suppressor. Cell. 72:791-800.
35. Turunen, O., T. Wahlström, and A. Vaheri. 1994. Ezrin has a COOH-terminal actin-binding site that is conserved in the ezrin protein family. J. Cell Biol. 126:1445-1453.
36. Ward, R.E.I., R.S. Lamb, and R.G. Fehon. 1998. A conserved functional domain of Drosophila Coracle is required for localisation at the septate junction and has membrane organizing activity. J. Cell Biol. 140:1463-1474.
37. Welling, D.B., M. Guida, F. Goll, D.K. Pearl, M.E. Glasscock, D.O. Pappas, F.H. Linthicum, D. Rogers, and T.W. Prior. 1996. Mutational spectrum in the neurofibromatosis type 2 gene in sporadic and familial schwannomas. Hum. Genet. 98:189-193.
38. Woods, D.F., and P.J. Bryant. 1993. Apical junctions and cell signalling in epithelia. J Cell Sci. 17(Suppl.):171-181.
39. Xu, T., and G. Rubin. 1993. Analysis of genetic mosaics in developing and adult Drosophila tissues. Development (Camb.). 117:1223-1237.
40. Xu, T., W. Wang, S. Zhang, R.A. Stewart, and W. Yu. 1995. Identifying tumor suppressors in genetic mosaics: the Drosophila lats gene encodes a putative protein kinase. Development (Camb.). 121:1053-1063.