Structure and function relationship of toxin from Chinese scorpion Buthus martensii Karsch (BmKAGAP): Gaining insight into related sites of analgesic activity

Peptides

Volumn 31, Issue 6, 2010, Pages 995-1000

  Cui, Yong ^a   Guo, Gui Li ^a   Ma, Lin ^a   Hu, Nan ^a   Song, Yong Bo ^a   Liu, Yan Feng ^a   Wu, Chun Fu ^a   Zhang, Jing Hai ^a  

^a SHENYANG PHARMACEUTICAL UNIVERSITY   (China)

Author keywords

Analgesic activity; BmKAGAP; Circular dichroism; Homology modeling; Site directed mutagenesis; Structure function relationship

Indexed keywords

RECOMBINANT ANTITUMOR ANALGESIC PEPTIDE; RECOMBINANT PROTEIN; SCORPION VENOM; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ANALGESIC ACTIVITY; ANIMAL EXPERIMENT; ARTICLE; BUTHUS MARTENSII; CIRCULAR DICHROISM; CONTROLLED STUDY; EPITOPE MAPPING; FEMALE; MALE; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SCORPION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANALGESICS; ANIMALS; ANTINEOPLASTIC AGENTS; CIRCULAR DICHROISM; MICE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDES; PROTEIN STRUCTURE, SECONDARY; SCORPION VENOMS; SCORPIONS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; STRUCTURE-ACTIVITY RELATIONSHIP;

ESCHERICHIA COLI; MESOBUTHUS MARTENSII; MUS;

EID: 77952109445     PISSN: 01969781     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.peptides.2010.03.017     Document Type: Article

References (23)

1. + channels in pain pathways. Trends Pharmacol Sci 22 (2001) 27-31
2. Benkhadir K., Kharrat R., Cestele S., Mosbah A., Rochat H., El Ayeb M., et al. Molecular cloning and functional expression of the alpha-scorpion toxin BotIII: pivotal role of the C-terminal region for its interaction with voltage-dependent sodium channels. Peptides 25 (2004) 151-161
3. Bessette P.H., Aslund F., Beckwith J., and Georgiou G. Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm. Proc Natl Acad Sci USA 96 (1999) 13703-13708
4. El Ayeb M., Darbon H., Bahraoui E.M., Vargas O., and Rochat H. Differential effects of defined chemical modifications on antigenic and pharmacological activities of scorpion alpha and beta toxins. Eur J Biochem 155 (1986) 289-294
5. Fennessy M.R., and Lee J.R. In: Ehrenpreis S., and Neidle A. (Eds). Methods in narcotics research (1975), Marcel Dekker, New York 76-79
6. Fontecilla-Camps J.C., Habersetzer-Rochat C., and Rochat H. Orthorhombic crystals and three-dimensional structure of the potent toxin II from the scorpion Androctonus australis Hector. Proc Natl Acad Sci USA 85 (1988) 7443-7447
7. Housset D., Habersetzer-Rochat C., Astier J.P., and Fontecilla-Camps J.C. Crystal structure of toxin II from the scorpion Androctonus australis Hector refined at 1.3 Å resolution. J Mol Biol 238 (1994) 88-103
8. Julius D., and Basbaum A.I. Molecular mechanisms of nociception. Nature 413 (2001) 203-210
9. Karbat I., Frolow F., Froy O., Gilles N., Cohen L., Turkov M., et al. Molecular basis of the high insecticidal potency of scorpion alpha-toxins. J Biol Chem 279 (2004) 31679-31686
10. Karbat I., Kahn R., Cohen L., Ilan N., Gilles N., Corzo G., et al. The unique pharmacology of the scorpion alpha-like toxin Lqh3 is associated with its flexible C-tail. FEBS J 274 (2007) 1918-1931
11. Kharrat R., Darbon H., Rochat H., and Granier C. Structure/activity relationships of scorpion alpha-toxins: multiple residues contribute to the interaction with receptors. Eur J Biochem 181 (1989) 381-390
12. Laskowski R.A., MacArthur M.W., Moss D.S., and Thornton J.M. PROCHECK: a program to check the stereochemical quality of protein structures. J Appl Cryst 26 (1993) 283-291
13. Legros C., Ceard B., Vacher H., Marchot P., Bougis P.E., and Martin-Eauclaire M.F. Expression of the standard scorpion alpha-toxin AaH II and AaH II mutants leading to the identification of some key bioactive elements. Biochim Biophys Acta 1723 (2005) 91-99
14. Lindahl E., Hess B., and van der Spoel D. GROMACS 3.0: a package for molecular simulation and trajectory analysis. J Mol Model 7 (2001) 306-317
15. + channels. J Membr Biol 158 (1997) 187-196
16. Liu Y.F., Ma R.L., Wang S.L., Duan Z.Y., Zhang J.H., Wu L.J., et al. Expression of an antitumor-analgesic peptide from the venom of Chinese scorpion Buthus martensii karsch in Escherichia coli. Protein Expr Purif 27 (2003) 253-258
17. Menez A. Functional architectures of animal toxins: a clue to drug design?. Toxicon 36 (1998) 1557-1572
18. + channel. Neuron 16 (1996) 123-130
19. Sali A., and Blundell T.L. Comparative protein modelling by satisfaction of spatial restraints. J Mol Biol 234 (1993) 779-815
20. Schnur E., Turkov M., Kahn R., Gordon D., Gurevitz M., and Anglister J. NMR analysis of interaction of LqhalphaIT scorpion toxin with a peptide corresponding to the D4/S3-S4 loop of insect para voltage-gated sodium channel. Biochemistry 47 (2008) 911-921
21. Sun Y.M., Bosmans F., Zhu R.H., Goudet C., Xiong Y.M., Tytgat J., et al. Importance of the conserved aromatic residues in the scorpion alpha-like toxin BmK M1: the hydrophobic surface region revisited. J Biol Chem 278 (2003) 24125-24131
22. Wang C.G., Gilles N., Hamon A., Le Gall F., Stankiewicz M., Pelhate M., et al. Exploration of the functional site of a scorpion alpha-like toxin by site-directed mutagenesis. Biochemistry 42 (2003) 4699-4708
23. Zhu J., Tong X.T., Cao C.Y., Wu G., Zhang N.X., and Wu H.M. Solution structure of BmKαTx11, a toxin from the venom of the Chinese scorpion Buthus martensii Karsch. Biochem Biophys Res Commun 391 (2010) 627-633