A high-throughput, cell-based screening method for siRNA and small molecule inhibitors of mTORC1 signaling using the in cell western technique

Assay and Drug Development Technologies

Volumn 8, Issue 2, 2010, Pages 186-199

  Hoffman, Gregory R ^a   Moerke, Nathan J ^a   Hsia, Max ^a   Shamu, Caroline E ^a   Blenis, John ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MAMMALIAN TARGET OF RAPAMYCIN; MAMMALIAN TARGET OF RAPAMYCIN COMPLEX 1; MAMMALIAN TARGET OF RAPAMYCIN COMPLEX 2; RAPAMYCIN; SMALL INTERFERING RNA;

ARTICLE; CELL GROWTH; CELL PROLIFERATION; CELL SURVIVAL; CONTROLLED STUDY; CYTOTOXICITY; DRUG ACTIVITY; DRUG EFFICACY; DRUG SAFETY; GENE ACTIVATION; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; HYDROPHOBICITY; IMMUNOFLUORESCENCE; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN MOTIF; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; TUMOR VOLUME; VALIDATION PROCESS;

AMINES; ANTIBODY SPECIFICITY; BLOTTING, WESTERN; CELL COUNT; CELL SURVIVAL; DRUG EVALUATION, PRECLINICAL; ENDPOINT DETERMINATION; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; HELA CELLS; HUMANS; INFRARED RAYS; REPRODUCIBILITY OF RESULTS; RNA, SMALL INTERFERING; SIGNAL TRANSDUCTION; SMALL MOLECULE LIBRARIES; TRANSCRIPTION FACTORS; TRANSFECTION;

EID: 77952046680     PISSN: 1540658X     EISSN: None     Source Type: Journal    
DOI: 10.1089/adt.2009.0213     Document Type: Article

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