Construction and expression of a dual vector for chemo-enzymatic synthesis of plant indole alkaloids in Escherichia coli

Natural Product Research

Volumn 24, Issue 8, 2010, Pages 759-766

  Stöckigt, Joachim ^a,b   Hammes, Bodo ^b   Ruppert, Martin ^b  

^a ZHEJIANG UNIVERSITY   (China)

^b JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

Chemo enzymatic synthesis; Dual vector pQE 70 STR1 SG; Engineered Escherichia coli; Indole alkaloids; Rauvolfia serpentina

Indexed keywords

CATHENAMINE; CATHENAMINE REDUCTASE; COMPLEMENTARY DNA; INDOLE ALKALOID; KPNI PROTEIN; OXIDOREDUCTASE; RESTRICTION ENDONUCLEASE; SECOLOGANIN; SODIUM BOROHYDRIDE; SPHI PROTEIN; STRICTOSIDINE; STRICTOSIDINE GLUCOSIDASE; STRICTOSIDINE SYNTHASE; TETRAHYDROALSTONINE; TRYPTAMINE; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CELL; BACTERIAL GENE; BACTERIAL STRAIN; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME ISOLATION; ENZYME PURIFICATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE CONSTRUCT; GENE EXPRESSION; INCUBATION TIME; INDIAN; MEDICINAL PLANT; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; PURIFICATION; RAUVOLFIA SERPENTINA;

CARBON-NITROGEN LYASES; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; GLUCOSIDASES; INDOLE ALKALOIDS; MOLECULAR STRUCTURE; PLANT PROTEINS; RAUWOLFIA; SECOLOGANIN TRYPTAMINE ALKALOIDS;

ESCHERICHIA COLI; RAUVOLFIA SERPENTINA;

EID: 77951987057     PISSN: 14786419     EISSN: 14786427     Source Type: Journal    
DOI: 10.1080/14786410903247304     Document Type: Article

References (10)

1. Bertani, G. (1951). Studies on lysogenesis: I. The mode of phage liberation by lysogenic Escherichia coli. Journal of Bacteriology, 62, 293-300.
2. Bertani, G. (2004). Lysogeny at mid-twentieth century: P1, P2, and other experimental systems. Journal of Bacteriology, 186, 595-600.
3. Chemler, J. A., & Koffas, M. A. G. (2008). Metabolic engineering for plant natural product biosynthesis in microbes. Current Opinion in Biotechnology, 19, 597-605.
4. Gao, S., von Schumann, G., & Stöckigt, J. (2002). A newly-detected reductase from Rauvolfia closes a gap in the biosynthesis of the antiarrhythmic alkaloid ajmaline. Planta Medica, 68, 906-911.
5. Gerasimenko, I., Sheludko, Y., Ma, X., & Stöckigt, J. (2002). Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. European Journal of Biochemistry, 269, 2204-2213.
6. Kutchan, T. M. (1995). Alkaloid biosynthesis - The basis for metabolic engineering of medicinal plants. Plant Cell, 7, 1059-1070.
7. Kutchan, T. M., Hampp, N., Lottspeich, F., Beyreuther, K., & Zenk, M. H. (1988). The cDNA clone for strictosidine synthase from Rauvolfia serpentina: DNA sequence determination and expression in E. coli. FEBS Letters, 237, 40-44.
8. Loris, E. A., Panjikar, S., Ruppert, M., Barleben, L., Unger, M., Schubel, H., et al. (2007). Structure-based engineering of strictosidine synthase: Auxiliary for alkaloid libraries. Chemistry & Biology, 14, 979-985.
9. Minami, H., Kim, J.-S., Ikezawa, N., Takemura, T., Katayama, T., Kumagai, H., et al. (2008). Microbial production of plant benzylisoquinoline alkaloids. Proceedings of the National Academy of Science USA, 105, 7393-7398.
10. Ruppert, M., Ma, X. Y., & Stöckigt, J. (2005). Alkaloid biosynthesis in Rauvolfia - cDNA cloning of major enzymes of the ajmaline pathway; Thematic issue 'Recent Progress in Alkaloid Chemistry'. Current Organic Chemistry, 9, 1431-1444.