A novel prokaryotic vector for identification and selection of recombinants: Direct use of the vector for expression studies in E. coli

Microbial Cell Factories

Volumn 9, Issue , 2010, Pages

  Banerjee, Sampali ^a   Kumar, Jitendra ^a   Apte Deshpande, Anjali ^a   Padmanabhan, Sriram ^a  

^a LUPIN LIMITED   (India)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL CELL; ESCHERICHIA COLI; EXPRESSION VECTOR; FLUORESCENCE ANALYSIS; GENE CONSTRUCT; GENE EXPRESSION; GENE INSERTION; GENE SWITCHING; GENETIC SCREENING; GENETIC TRANSFORMATION; MOLECULAR CLONING; NONHUMAN; PROKARYOTE; PROTEIN TARGETING; RECOMBINANT GENE; REPORTER GENE; BIOSYNTHESIS; GENE VECTOR; GENETIC PROCEDURES; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; SOLUBILITY;

BACTERIA (MICROORGANISMS); PROKARYOTA;

CLONING, MOLECULAR; ESCHERICHIA COLI; GENES, REPORTER; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; MOLECULAR PROBE TECHNIQUES; RECOMBINANT PROTEINS; SOLUBILITY;

EID: 77951969143     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-9-30     Document Type: Article

References (21)

1. Gronenborn B, Messing J. Methylation of single stranded DNA in vitro introduces new restriction endonuclease cleavage sites. Nature 1978, 272:375-377. 10.1038/272375a0, 416358.
2. Bernard P, Gabant P, Bahassi EM, Couturier M. Positive-selection vectors using the F plasmid ccdB killer gene. Gene 1994, 148:71-74. 10.1016/0378-1119(94)90235-6, 7926841.
3. Bernard P, Couturier M. The 41 carboxy-terminal residues of the miniF plasmid CcdA protein are sufficient to antagonize the killer activity of the CcdB protein. Mol Gen Genet 1991, 226:297-304. 10.1007/BF00273616, 2034222.
4. Bernard P, Couturier M. Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes. J Mol Biol 1992, 226:735-45. 10.1016/0022-2836(92)90629-X, 1324324.
5. Mandi N, Kotwal P, Padmanabhan S. Construction of a novel zero background prokaryotic expression vector: potential advantages. Biotechnol Lett 2009, 31:1905-1910. 10.1007/s10529-009-0090-6, 19690807.
6. Patterson GH, Knobel SM, Sharif WD, Kain SR, Piston DW. Use of green fluorescent protein and its mutants in quantitative fluorescence microscopy. Biophysical Journal 1997, 73:2782-2790. 10.1016/S0006-3495(97)78307-3, 1181180, 9370472.
7. Prasher DC, Eckenrode VK, Ward WW, Prendergast FG, Cormier MJ. Primary structure of Aqueorea Victoria green fluorescent protein. Gene 1992, 111:229-233. 10.1016/0378-1119(92)90691-H, 1347277.
8. Chalfie M, Euskirche YTG, Ward WW, Prasher DC. Green fluorescent protein as a marker for gene expression. Science 1994, 263:802-805. 10.1126/science.8303295, 8303295.
9. Inouye S, Tsuji FI. Aqueorea green fluorescent protein expression of the gene and fluorescent characteristics of the recombinant protein. FEBS Letters 1994, 341:277-280. 10.1016/0014-5793(94)80472-9, 8137953.
10. Yang F, Larry GM, George NP. The molecular structure of green fluorescent protein. Nature Biotechnology 1996, 14:1246-1251. 10.1038/nbt1096-1246, 9631087.
11. Inouye S, Hidesato O, Yasuda K, Umesono K, Tsuji FI. A bacterial cloning vector using a mutated Aequorea green fluorescent protein as an indicator. Gene 1997, 189:159-162. 10.1016/S0378-1119(96)00753-6, 9168121.
12. Davis SJ, Vierstra RD. Soluble, highly fluorescent variants of green fluorescent protein (GFP) for use in higher plants. Plant Molecular Biology 1998, 36:581-8.
13. Shi H, Wen Su W. Display of green fluorescent protein on Escherichia coli cell surface. Enzyme Microb Technol 2001, 28:25-34. 10.1016/S0141-0229(00)00281-7, 11118595.
14. Stathopoulous C, Provence DL, Curtiss R. Characterization of the Avian pathogenic E. coli heamagglutinin Tsh, a member of immunoglobulin A protease-type family for autotransporters. Infection and Immunology 1999, 67:772-781.
15. Ogawa H, Inouye S, Tsuji FI, Yasuda K, Umesono K. Localization, trafficking, and temperature-dependence of Aequorea green fluorescent protein in cultured vertebrate cells. Proceeding of the National Academy of Science, USA 1995, 92:11899-11903.
16. Beneyx F, Georgiou G. In vivo degradation of secreted fusion proteins by the E. coli outer membrane protease OmpT. Journal of Bacteriology 1990, 172:491-494. 208460, 2403549.
17. Davis SJ, Vierstra RD. Soluble, highly fluorescent variants of green fluorescent protein (GFP) for use in higher plants. Plant Molecular Biology 1998, 36:581-8.
18. Rabbani SA, Yasuda T, Bennett HP, Sung WL, Zahab DM, Tam CS, Goltzman D, Hendy GN. Recombinant human parathyroid hormone synthesised in E. coli. Purification and characterization. J Biol Chem 1988, 263:1307-1313.
19. Banerjee S, Salunkhe SS, Apte-Deshpande AD, Mandi SN, Mandal G, Padmanabhan S. Over-expression of proteins using a modified pBAD24 vector in E. coli expression system. Biotechnol Lett 2009, 31:1031-1036. 10.1007/s10529-009-9976-6, 19330488.
20. Mandi N, Soorapaneni S, Rewanwar S, Kotwal P, Prasad B, Mandal G, Padmanabhan S. High yielding recombinant Staphylokinase in bacterial expression system--cloning, expression, purification and activity studies. Protein Expr Purif 2009, 64:69-75. 10.1016/j.pep.2008.10.010, 19000928.
21. Baird GS, Zacharias DA, Tsien RY. Biochemistry, mutagenesis, and oligomerization of DSRed, a red fluorescent protein from coral. PNAS USA 2000, 97:11984-11989. 10.1073/pnas.97.22.11984, 17281, 11050229.