High yielding recombinant Staphylokinase in bacterial expression system-cloning, expression, purification and activity studies

Protein Expression and Purification

Volumn 64, Issue 1, 2009, Pages 69-75

  Mandi, Nagnath ^a   Soorapaneni, Sudheerbabu ^a   Rewanwar, Sachin ^a   Kotwal, Prakash ^a   Prasad, Bhaskarjyoti ^a   Mandal, Goutam ^a   Padmanabhan, Sriram ^a  

^a LUPIN LIMITED   (India)

Author keywords

Chromogenic substrate assay; Cloning; Expression; PG activation; Staphylococcus aureus; Staphylokinase; T7 promoter

Indexed keywords

AUR PROTEIN, STAPHYLOCOCCUS AUREUS; ISOPROPYL THIOGALACTOSIDE; METALLOPROTEINASE; PLASMINOGEN; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL GENE; BASE PAIRING; BIOREACTOR; CHEMISTRY; ENZYMOLOGY; FERMENTATION; GENE EXPRESSION REGULATION; GENE VECTOR; GENETICS; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; MICROBIOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; PEPTIDE MAPPING; SEQUENCE HOMOLOGY; SOLUBILITY; STAPHYLOCOCCUS AUREUS;

BASE PAIRING; BASE SEQUENCE; BIOREACTORS; CHROMATOGRAPHY, ION EXCHANGE; CLONING, MOLECULAR; FERMENTATION; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; GENETIC VECTORS; ISOPROPYL THIOGALACTOSIDE; METALLOENDOPEPTIDASES; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PEPTIDE MAPPING; PLASMINOGEN; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, NUCLEIC ACID; SOLUBILITY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STAPHYLOCOCCUS AUREUS;

BACTERIA (MICROORGANISMS); STAPHYLOCOCCUS AUREUS;

EID: 57749121865     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.10.010     Document Type: Article

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