Using fluorometry and ion-sensitive microelectrodes to study the functional expression of heterologously-expressed ion channels and transporters in Xenopus oocytes

Methods

Volumn 51, Issue 1, 2010, Pages 134-145

  Musa Aziz, Raif ^b   Boron, Walter F ^a   Parker, Mark D ^a  

^a CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

EGFP; Intracellular pH; Ion sensitive cocktail; ISE; pGH19; pHi; Typhoon

Indexed keywords

CARRIER PROTEIN; CHLORIDE ION; COMPLEMENTARY RNA; GREEN FLUORESCENT PROTEIN; ION CHANNEL; MESSENGER RNA; POTASSIUM ION; PROTON; SODIUM ION; ION;

CELL MEMBRANE; CELL SIZE; ELECTROPHYSIOLOGY; FLUOROMETRY; HETEROLOGOUS EXPRESSION; ION TRANSPORT; MICROELECTRODE; MICROINJECTION; NONHUMAN; OOCYTE; PRIORITY JOURNAL; PROTEIN PROCESSING; REVIEW; TRANSPORT KINETICS; XENOPUS LAEVIS; ANIMAL; CALIBRATION; HUMAN; METABOLISM; PROCEDURES; TRANSPORT AT THE CELLULAR LEVEL;

ANIMALS; BIOLOGICAL TRANSPORT; CALIBRATION; CELL MEMBRANE; ELECTROPHYSIOLOGY; FLUOROMETRY; GREEN FLUORESCENT PROTEINS; HUMANS; ION CHANNELS; IONS; MICROELECTRODES; OOCYTES; RNA, COMPLEMENTARY; RNA, MESSENGER; XENOPUS LAEVIS;

XENOPUS LAEVIS;

EID: 77951877266     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2009.12.012     Document Type: Review

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