Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36

Extremophiles

Volumn 14, Issue 2, 2010, Pages 185-192

  Visser, Daniel F ^a   Hennessy, Fritha ^a   Rashamuse, Konanani ^a   Louw, Maureen E ^b   Brady, Dean ^a  

^a CSIR Biosciences   (South Africa)

^b DSI CSIR Nanotechnology Innovation Centre   (South Africa)

Author keywords

5 Methyluridine; Bacillus halodurans; Biocatalysis; Guanosine; Nucleoside phosphorylase

Indexed keywords

BACTERIAL DNA; PRIMER DNA; PURINE NUCLEOSIDE PHOSPHORYLASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS; BACTERIAL GENE; CHEMICAL STRUCTURE; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PH; PROTEIN TERTIARY STRUCTURE; SEQUENCE HOMOLOGY; STRUCTURAL HOMOLOGY; THERMODYNAMICS;

AMINO ACID SEQUENCE; BACILLUS; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; DNA, BACTERIAL; ESCHERICHIA COLI; GENES, BACTERIAL; HYDROGEN-ION CONCENTRATION; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, TERTIARY; PURINE-NUCLEOSIDE PHOSPHORYLASE; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; STRUCTURAL HOMOLOGY, PROTEIN; THERMODYNAMICS;

BACILLUS HALODURANS; ESCHERICHIA COLI;

EID: 77951767108     PISSN: 14310651     EISSN: 14334909     Source Type: Journal    
DOI: 10.1007/s00792-009-0297-4     Document Type: Article

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