SH-SY5Y human neuroblastoma cell line: In vitro cell model of dopaminergic neurons in Parkinson's disease

Chinese Medical Journal

Volumn 123, Issue 8, 2010, Pages 1086-1092

  Xie, Hong Rong ^a   Hu, Lin Sen ^a   Li, Guo Yi ^a  

^a JILIN UNIVERSITY   (China)

Author keywords

Cell model; Differentiation; Dopaminergic neuron; Parkinson's disease; Sh sy5y cells

Indexed keywords

1 METHYL 4 PHENYLPYRIDINIUM; DOPAMINE BETA MONOOXYGENASE; DOPAMINE TRANSPORTER; NEUROPROTECTIVE AGENT; NEUROTOXIN; NEUROTROPHIN; PHORBOL 13 ACETATE 12 MYRISTATE; RETINOIC ACID; STAUROSPORINE; TYROSINE 3 MONOOXYGENASE;

CELL COUNT; CELL KINETICS; CELL PROLIFERATION; CHINESE; DOPAMINERGIC NERVE CELL; DOPAMINERGIC TRANSMISSION; HUMAN; IN VITRO STUDY; NERVE CELL DIFFERENTIATION; NEUROBLASTOMA CELL; NEUROPROTECTION; NEUROTOXICITY; PARKINSON DISEASE; PHENOTYPE; PROTEIN EXPRESSION; REVIEW;

CELL DIFFERENTIATION; CELL LINE, TUMOR; DOPAMINE; HUMANS; NEUROBLASTOMA; NEURONS; PARKINSON DISEASE;

EID: 77951240010     PISSN: 03666999     EISSN: None     Source Type: Journal    
DOI: 10.3760/cma.j.issn.0366-6999.2010.08.021     Document Type: Review

References (60)

1. Koch A, Lehmann-Horn K, Dächsel JC, Gasser T, Kahle PJ, Lücking CB. Proteasomal inhibition reduces parkin mRNA in PC12 and SH-SY5Y cells. Parkinsonism Relat Disord 2009; 15: 220-225.
2. Cheng YF, Zhu GQ, Wang M, Cheng H, Zhou A, Wang N, et al. Involvement of ubiquitin proteasome system in protective mechanisms of Puerarin to MPP(+)-elicited apoptosis. Neurosci Res 2009; 63: 52-58.
3. Takeuchi H, Yanagida T, Inden M, Takata K, Kitamura Y, Yamakawa K, et al. Nicotinic receptor stimulation protects nigral dopaminergic neurons in rotenone-induced Parkinson's disease models. J Neurosci Res 2009; 87: 576-585.
4. Biedler JL, Helson L, Spengler BA. Morphology and growth, tumorigenicity and cytogenetics of human neuroblastoma cells in continuous culture. Cancer Res 1973; 33: 2643-2652.
5. Joshi S, Guleria R, Pan J, DiPette D, Singh US. Retinoic acid receptors and tissue-transglutaminase mediate short-term effect of retinoic acid on migration and invasion of neuroblastoma SH-SY5Y cells. Oncogene 2006; 25: 240-247
6. Ciccarone V, Spengler BA, Meyers MB, Biedler JL, Ross RA. Phenotypic diversification in human neuroblastoma cells: expression of distinct neural crest lineages. Cancer Res 1989; 49: 219-225.
7. Singh J, Kaur G. Transcriptional regulation of polysialylated neural cell adhesion molecule expression by NMDA receptor activation in retinoic acid-differentiated SH-SY5Y neuroblastoma cultures. Brain Res 2007; 1154: 8-21.
8. Påhlman S, Odelstad L, Larsson E, Grotte G, Nilsson K. Phenotypic changes of human neuroblastoma cells in culture induced by 12-O-tetradecanoyl-phorbol-13-acetate. Int J Cancer 1981; 28: 583-589.
9. Cernaianu G, Brandmaier P, Scholz G, Ackermann OP, Alt R, Rothe K, et al. All-trans retinoic acid arrests neuroblastoma cells in a dormant state. Subsequent nerve growth factor/brain derived neurotrophic factor treatment adds modest benefit. J Pediatr Surg 2008; 43: 1284-1294.
10. Kume T, Kawato Y, Osakada F, Izumi Y, Katsuki H, Nakagawa T, et al. Dibutyryl cyclic AMP induces differentiation of human neuroblastoma SH-SY5Y cells into a noradrenergic phenotype. Neurosci Lett 2008; 443: 199-203.
11. Guarnieri S, Pilla R, Morabito C, Sacchetti S, Mancinelli R, Fanò G, et al. Extracellular guanosine and GTP promote expression of differentiation markers and induce S-phase cell-cycle arrest in human SH-SY5Y neuroblastoma cells. Int J Dev Neurosci 2009; 27: 135-147.
12. Mollereau C, Zajac JM, Roumy M. Staurosporine differentiation of NPFF2 receptor-transfected SH-SY5Y neuroblastoma cells induces selectivity of NPFF activity towards opioid receptors. Peptides 2007; 28: 1125-1128.
13. Oyarce AM, Fleming PJ. Multiple forms of human dopamine beta-hydroxylase in SH-SY5Y neuroblastoma cells. Arch Biochem Biophys 1991; 290: 503-510.
14. Takahashi T, Deng Y, Maruyama W, Dostert P, Kawai M, Naoi M. Uptake of a neurotoxin-candidate, (R)-1,2-dimethyl-6, 7-dihydroxy-1,2,3,4-tetrahydroisoquinoline into human dopaminergic neuroblastoma SH-SY5Y cells by dopamine transport system. J Neural Transm Gen Sect 1994; 98: 107-118.
15. Cheung YT, Lau WK, Yu MS, Lai CS, Yeung SC, So KF, et al. Effects of all-trans-retinoic acid on human SH-SY5Y neuroblastoma as in vitro model in neurotoxicity research. Neurotoxicology 2009; 30: 127-135.
16. Datki Z, Juhász A, Gálfi M, Soós K, Papp R, Zádori D, et al. Method for measuring neurotoxicity of aggregating polypeptides with the MTT assay on differentiated neuroblastoma cells. Brain Res Bull 2003; 62: 223-229.
17. Presgraves SP, Ahmed T, Borwege S, Joyce JN. Terminally differentiated SH-SY5Y cells provide a model system for studying neuroprotective effects of dopamine agonists. Neurotox Res 2004; 5: 579-598.
18. Storch A, Burkhardt K, Ludolph AC, Schwarz J. Protective effects of riluzole on dopamine neurons: involvement of oxidative stress and cellular energy metabolism. J Neurochem 2000; 75: 2259-2269.
19. Ikeda H, Pastuszko A, Ikegaki N, Kennett RH, Wilson DF. 3,4-dihydroxyphenylalanine (dopa) metabolism and retinoic acid induced differentiation in human neuroblastoma. Neurochem Res 1994; 19: 1487-1494.
20. Fagerström S, Påhlman S, Gestblom C, Nånberg E. Protein kinase C-epsilon is implicated in neurite outgrowth in differentiating human neuroblastoma cells. Cell Growth Differ 1996; 7: 775-785.
21. Gimenez-Cassina A, Lim F, Diaz-Nido J. Differentiation of a human neuroblastoma into neuron-like cells increases their susceptibility to transduction by herpesviral vectors. J Neurosci Res 2006; 84: 755-767.
22. Åkerman KEO, Scott IG, Andersson LC. Functional differentiation of human ganglion cell derived neuroblastoma cell line SH-SY5Y induced by a phorbolester (TPA). Neurochem Int 1984; 6: 77-80.
23. Tosetti P, Taglietti V, Toselli M. Functional changes in potassium conductances of the human neuroblastoma cell line SH-SY5Y during in vitro differentiation. J Neurophysiol 1998; 79: 648-658.
24. 2+ entry after retinoic acid-induced differentiation of neuroblastoma cells. Biochem J 2005; 388: 941-948.
25. Millan MJ, Di Cara B, Hill M, Jackson M, Joyce JN, Brotchie J, et al. S32504, a novel naphtoxazine agonist at dopamine D3/D2 receptors: II. Actions in rodent, primate and cellular models of antiparkinson activity in comparison to ropinirole. J Pharmacol Exp Ther 2004; 309: 921-935.
26. Jenab S, Inturrisi CE. Retinoic acid regulation of mu opioid receptor and c-fos mRNAs and AP-1 DNA binding in SH-SY5Y neuroblastoma cells. Molecular. Brain Res Mol Brain Res 2002; 99: 34-39.
27. Scheller D, Ullmer C, Berkels R, Gwarek M, Lübbert H. The in vitro receptor profile of rotigotine: a new agent for the treatment of Parkinson's disease. Naunyn Schmiedebergs Arch Pharmacol 2009; 379: 73-86
28. Presgraves SP, Borwege S, Millan MJ, Joyce JN. Involvement of dopamine D2/D3 receptors and BDNF in the neuroprotective effects of S32504 and pramipexole against 1-methyl-4-phenylpyridinium in terminally differentiated SH-SY5Y cells. Exp Neurol 2004; 190: 157-170.
29. Farooqui SM. Induction of adenylate cyclase sensitive dopamine D2-receptors in retinoic acid induced differentiated human neuroblastoma SHSY-5Y cells. Life Sci 1994; 55: 1887-1893.
30. Påhlman S, Ruusala AI, Abrahamsson L, Mattsson ME, Esscher T. Retinoic acid-induced differentiation of cultured human neuroblastoma cells: a comparison with phorbolester-induced differentiation. Cell Differ 1984; 14: 135-144.
31. Clagett-Dame M, McNeill EM, Muley PD. Role of all-trans retinoic acid in neurite outgrowth and axonal elongation. J Neurobiol 2006; 66: 739-756.
32. Uemura K, Kitagawa N, Kohno R, Kuzuya A, Kageyama T, Shibasaki H, et al. Presenilin 1 mediates retinoic acid-induced differentiation of SH-SY5Y cells through facilitation of Wnt signaling. J Neurosci Res 2003; 73: 166-175.
33. Kim SN, Kim SG, Park SD, Cho-Chung YS, Hong SH. Participation of type II protein kinase A in the retinoic acid-induced growth inhibition of SH-SY5Y human neuroblastoma cells. J Cell Physiol 2000; 182: 421-428.
34. Encinas M, Iglesias M, Liu Y, Wang H, Muhaisen A, Ceña V, et al. Sequential treatment of SH-SY5Y cells with retinoic acid and brain-derived neurotrophic factor gives rise to fully differentiated, neurotrophic factor dependent, human neuron-like cells. J Neurochem 2000; 75: 991-1003.
35. Pennypacker KR, Kuhn DM, Billingsley ML. Changes in expression of tyrosine hydroxylase immunoreactivity in human SMS-KCNR neuroblastoma following retinoic acid or phorbol ester-induced differentiation. Brain Res Mol Brain Res 1989; 5: 251-258.
36. Adem A, Mattsson ME, Nordberg A, Påhlman S. Muscarinic receptors in human SH-SY5Y neuroblastoma cell line: regulation by phorbol ester and retinoic acid-induced differentiation. Brain Res 1987; 430: 235-242.
37. Magni P, Beretta E, Scaccianoce E, Motta M. Retinoic acid negatively regulates neuropeptide Y expression in human neuroblastoma cells. Neuropharmacology 2000; 39: 1628-1636.
38. Sarkanen JR, Nykky J, Siikanen J, Selinummi J, Ylikomi T, Jalonen TO. Cholesterol supports the retinoic acid-induced synaptic vesicle formation in differentiating human SH-SY5Y neuroblastoma cells. J Neurochem 2007; 102: 1941-1952.
39. Mattsson ME, Ruusala AI, Påhlman S. Changes in inducibility of ornithine decarboxylase activity in differentiating human neuroblastoma cells. Exp Cell Res 1984; 155: 105-112.
40. Heby O. Role of polyamines in the control of cell proliferation and differentiation. Differentiation 1981; 19: 1-20.
41. Mattsson ME, Enberg G, Ruusala AI, Hall K, Påhlman S. Mitogenic response of human SH-SY5Y neuroblastoma cells to insulin-like growth factor I and II is dependent on the stage of differentiation. J Cell Biol 1986; 102: 1949-1954.
42. Jalava A, Heikkilä J, Lintunen M, Akerman K, Påhlman S. Staurosporine induces a neuronal phenotype in SH-SY5Y human neuroblastoma cells that resembles that induced by the phorbol ester 12-O-tetradecanoyl phorbol-13 acetate (TPA). FEBS Lett 1992; 300: 114-118.
43. Gómez-Santos C, Ambrosio S, Ventura F, Ferrer I, Reiriz J. TGF-beta1 increases tyrosine hydroxylase expression by a mechanism blocked by BMP-2 in human neuroblastoma SH-SY5Y cells. Brain Res 2002; 958: 152-160.
44. Kaplan DR, Miller FD. Neurotrophin signal transduction in the nervous system. Curr Opin Neurobiol 2000: 381-391.
45. Lavenius E, Parrow V, Nånberg E, Påhlman S. Basic FGF and IGF-I promote differentiation of human SH-SY5Y neuroblastoma cells in culture. Growth Factors 1994; 10: 29-39.
46. Kou W, Luchtman D, Song C. Eicosapentaenoic acid (EPA) increases cell viability and expression of neurotrophin receptors in retinoic acid and brain-derived neurotrophic factor differentiated SH-SY5Y cells. Eur J Nutr 2008; 47: 104-113.
47. Edsjö A, Lavenius E, Nilsson H, Hoehner JC, Simonsson P, Culp LA, et al. Expression of trkB in human neuroblastoma in relation to MYCN expression and retinoic acid treatment. Lab Invest 2003; 83: 813-823.
48. Mastroeni D, Grover A, Leonard B, Joyce JN, Coleman PD, Kozik B, et al. Microglial responses to dopamine in a cell culture model of Parkinson's disease. Neurobiol Aging 2009; 30: 1805-1817.
49. Jalava A, Akerman K, Heikkilä J. Protein kinase inhibitor, staurosporine, induces a mature neuronal phenotype in SH-SY5Y human neuroblastoma cells through an alpha-, beta-, and zeta-protein kinase C-independent pathway. J Cell Physiol 1993; 155: 301-312.
50. Edsjö A, Holmquist L, Påhlman S. Neuroblastoma as an experimental model of neuronal differentiation and hypoxia-induced tumor cell dedifferentiation. Semin Cancer Biol 2007; 17: 248-256.
51. Shen JH, Zhang Y, Wu NH, Shen YF. Resistance to geldanamycin-induced apoptosis in differentiated neuroblastoma SH-SY5Y cells. Neurosci Lett 2007; 414: 110-114.
52. Cecchi C, Pensalfini A, Liguri G, Baglioni S, Fiorillo C, Guadagna S, et al. Differentiation increases the resistance of neuronal cells to amyloid toxicity. Neurochem Res 2008; 33: 2516-2531.
53. Lasorella A, Iavarone A, Israel MA. Differentiation of neuroblastoma enhances Bcl-2 expression and induces alterations of apoptosis and drug resistance. Cancer Res 1995; 55: 4711-4716.
54. Tieu K, Zuo DM, Yu PH. Differential effects of staurosporine and retinoic acid on the vulnerability of the SH-SY5Y neuroblastoma cells: involvement of Bcl-2 and p53 proteins. J Neurosci Res 1999; 58: 426-435.
55. Jantas D, Pytel M, Mozrzymas JW, Leskiewicz M, Regulska M, Antkiewicz-Michaluk L, et al. The attenuating effect of memantine on staurosporine-, salsolinol- and doxorubicin- induced apoptosis in human neuroblastoma SH-SY5Y cells. Neurochem Int 2008; 52: 864-877.
56. Ho R, Eggert A, Hishiki T, Minturn JE, Ikegaki N, Foster P, et al. Resistance of chemotherapy mediated by TrkB in neuroblastomas. Cancer Res 2002; 62: 6462-6466.
57. Guo JT, Chen AQ, Kong Q, Zhu H, Ma CM, Qin C. Inhibition of vesicular monoamine transporter-2 activity in a-synuclein stably transfected SH-SY5Y cells. Cell Mol Neurobiol 2008; 28: 35-47.
58. Lonergan PE, Martin DS, Horrobin DF, Lynch MA. Neuroprotective actions of eicosapentaenoic acid on lipopolysaccharide-induced dysfunction in rat hippocampus. J Neurochem 2004; 91: 20-29.
59. Kitao Y, Matsuyama T, Takano K, Tabata Y, Yoshimoto T, Momoi T, et al. Does ORP150/HSP12A protect dopaminergic neurons against MPTP/MPP(+)-induced neurotoxicity? Antioxid Redox Signal 2007; 9: 589-595.
60. Gainetdinov RR, Fumagalli F, Jones SR, Caron MG. Dopamine transporter is required for in vivo MPTP neurotoxicity: evidence from mice lacking the transporter. J Neurochem 1997; 69: 1322-1325.