Cloning, sequence analysis, and expression of a gene encoding Chromobacterium sp. DS-1 cholesterol oxidase

Applied Microbiology and Biotechnology

Volumn 82, Issue 3, 2009, Pages 479-490

  Doukyu, Noriyuki ^a   Shibata, Kanpei ^a   Ogino, Hiroyasu ^b   Sagermann, Martin ^c  

^a TOYO UNIVERSITY   (Japan)

^b Osaka Prefecture University   (Japan)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cholesterol oxidase; Chromobacterium; Cloning; Detergent; Organic solvent; Thermal stability

Indexed keywords

AMINO ACID RESIDUES; AMINO ACID SEQUENCES; BURKHOLDERIA; BURKHOLDERIA CEPACIA; CHOLESTEROL OXIDASE; CHROMOBACTERIUM; CIRCULAR DICHROISMS; CONFORMATIONAL STABILITIES; DNA FRAGMENTS; EXPRESSION VECTORS; EXTRACELLULAR CHOLESTEROL OXIDASE; HIGH TEMPERATURES; HIGHER TEMPERATURES; LINEWEAVER-BURK PLOTS; MATURE PRODUCTS; NATIVE ENZYMES; PHYSICO-CHEMICAL PROPERTIES; PSEUDOMONAS AERUGINOSA; RECOMBINANT E. COLI; SEQUENCE ANALYSIS; SPECTRAL ANALYSIS; STRUCTURAL GENES; THERMAL STABILITY;

AMINATION; AMINES; AMINO ACIDS; CHOLESTEROL; CLONING; DICHROISM; DIELECTRIC RELAXATION; ENCODING (SYMBOLS); ENZYMES; ESCHERICHIA COLI; GENE ENCODING; NUCLEIC ACIDS; ORGANIC ACIDS; ORGANIC SOLVENTS; PURIFICATION; SETTLING TANKS; SOAPS (DETERGENTS); SPECTRUM ANALYSIS; SPECTRUM ANALYZERS; THERMODYNAMIC STABILITY;

ENZYME IMMOBILIZATION;

ALCOHOL; AMINO ACID; BACTERIUM; DETERGENT; DNA; ENZYME ACTIVITY; GENE EXPRESSION; PHYSICOCHEMICAL PROPERTY; RECOMBINATION; SOLVENT; SPECTRAL ANALYSIS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; CHOLESTEROL OXIDASE; CHROMOBACTERIUM; CLONING, MOLECULAR; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; KINETICS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; SEQUENCE ANALYSIS;

BURKHOLDERIA; BURKHOLDERIA CEPACIA; CHROMOBACTERIUM; CHROMOBACTERIUM SP; ESCHERICHIA COLI; PSEUDOMONAS AERUGINOSA;

EID: 60549095397     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1775-9     Document Type: Article

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