Overexpression, one-step purification, and characterization of a type II cholesterol oxidase from a local isolate Rhodococcus sp. PTCC 1633

World Journal of Microbiology and Biotechnology

Volumn 25, Issue 5, 2009, Pages 773-779

  Ghasemian, Abdollah ^a   Yazdi, Mojtaba Tabatabaei ^a   Sepehrizadeh, Zargham ^a   Yazdi, Zohreh Tabatabaei ^a   Zarrini, Gholamreza ^b  

^a TEHRAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^b UNIVERSITY OF TABRIZ   (Iran)

Author keywords

Cholesterol oxidase; One step purification; Overexpression; Rhodococcus

Indexed keywords

ABSORPTION PEAKS; CELL LYSATE; CHOLESTEROL OXIDASE; ISO-ELECTRIC POINTS; ONE STEP-PURIFICATION; ONE-STEP PURIFICATIONS; OPTIMUM PH; OVEREXPRESSION; PURIFIED PROTEINS; RECOMBINANT ENZYMES; RHODOCOCCUS; RHODOCOCCUS SP; TYPE II;

ABSORPTION; CHOLESTEROL; ENCODING (SYMBOLS); ENZYMES; ESCHERICHIA COLI; PURIFICATION; SULFUR COMPOUNDS;

GENE ENCODING;

ESCHERICHIA COLI; RHODOCOCCUS; RHODOCOCCUS SP.;

EID: 64449088282     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-008-9948-3     Document Type: Article

References (26)

1. S Ahmad SK Garg BN Johri 1992 Biotransformation of sterols: selective cleavage of the side chain Biotechnol Adv 10 1 67 10.1016/0734-9750(92)91351-E
2. CC Allian LS Poon CSG Chan W Richmond PC Fu 1974 Enzymatic determination of total serum cholesterol Clin Chem 20 470 475
3. SF Altschul W Gissh W Miller EW Mysers DJ Lipman 1990 Basic local alignment search tool J Mol Biol 215 403 410
4. A Brzostek B Dziadek A Rumijowska-Galewicz J Pawelczyk J Dziadek 2007 Cholesterol oxidase is required for virulence of Mycobacterium tuberculosis FEMS Microbiol Lett 275 106 112 10.1111/j.1574-6968.2007.00865.x
5. DR Corbin JT Greenplate ED Wong JP Purcell 1994 Cloning of an insecticidal cholesterol oxidase gene and its expression in bacteria and in plant protoplasts Appl Environ Microbiol 60 4239 4244
6. R Coulombe KQ Yue S Ghisla A Vrielink 2001 Oxygen access to the active site of cholesterol oxidase through a narrow channel is gated by an Arg-Glu pair J Biol Chem 276 30435 30441 10.1074/jbc.M104103200
7. S Dieth D Tritsch J-F Biellmann 1995 Resolution of allylic alcohols by cholesterol oxidase isolated from Rhodococcus erythropolis Tetrahedron Lett 36 2243 2246 10.1016/0040-4039(95)00235-5
8. N Doukyu R Aono 2001 Cloning, sequence analysis and expression of a gene encoding an organic solvent- and detergent-tolerant cholesterol oxidase of Burkholderia cepacia strain ST-200 Appl Microbiol Biotechnol 57 146 152 10.1007/s002530100753
9. K Fujishiro T Ohta M Hasegawa K Yamaguchi T Mizukami T Uwajima 1990 Isolation and identification of the gene of cholesterol oxidase from Brevibacterium sterolicum ATCC21387, a widely used enzyme in clinical analysis Biochem Biophys Res Commun 172 721 727 10.1016/0006-291X(90)90734-5
10. K Fujishiro H Uchida K Shimokava M Nakano F Sano T Ohta N Nakahara K Aisak T Uwajima 2002 Purification and properties of a new Brevibacterium sterolicum cholesterol oxidase produced by E. coli MM294/pnH10 FEMS Microbiol Lett 215 243 248 10.1111/j.1574-6968.2002.tb11397.x
11. D Hanahan 1983 Studies on transformation of Escherichia coli with plasmids J Mol Biol 166 557 580 10.1016/S0022-2836(83)80284-8
12. UK Laemmli 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 680 685 10.1038/227680a0
13. J Li A Vrielink P Brick DMR Blow 1993 Crystal structure of cholesterol oxidase complexed with a steroid substrate: implications for flavin adenine dinucleotide dependent alcohol oxidases Biochemistry 32 11507 11515
14. J MacLachlan ATL Wotherspoon RO Ansell CJW Brooks 2000 Cholesterol oxidase: sources, physical properties and analytical applications J Steroid Biochem Mol Biol 72 169 195 10.1016/S0960-0760(00)00044-3
15. Y Murooka T Ishizaki O Nimi N Maekawa 1986 Cloning and expression of a Streptomyces sp. cholesterol oxidase gene in Streptomyces lividans with plasmid pIJ702 Appl Environ Microbiol 52 1382 1385
16. J Navas B Gonzalez-Zorn N Ladron P Garrido JA Vazquez-Boland 2001 Identification and mutagenesis by allelic exchange of choE, encoding a cholesterol oxidase from the intracellular pathogen Rhodococcus equi J Bacteriol 183 4796 4805 10.1128/JB.183.16.4796-4805.2001
17. A Noma K Nakayama 1976 Comparative studies on cholesterol oxidases from different sources Clin Chim Acta 73 487 496 10.1016/0009-8981(76)90152-2
18. Y Pei C Dupont T Sydor A Haas JF Prescott 2006 Cholesterol oxidase (ChoE) is not important in the virulence of Rhodococcus equi Vet Microbiol 118 240 246 10.1016/j.vetmic.2006.08.006
19. L Puglielli AL Friedlich KDR Setchell S Nagano C Opazo RA Cherny KJ Barnham JD Wade S Melov DM Kovacs AI Bush 2005 Alzheimer disease β-amyloid activity mimics cholesterol oxidase J Clin Invest 115 2556 2563 10.1172/JCI23610
20. JP Purcell JT Greenplate MG Jennings JS Ryerse JC Pershing SR Sims MJ Prinsen DR Corbin M Tran RD Sammons 1993 Cholesterol oxidase: a potent insecticidal protein active against boll weevil larvae Biochem Biophys Res Commun 196 1406 1413 10.1006/bbrc.1993.2409
21. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor
22. NS Sampson X Chen 1998 Increased expression of Brevibacterium sterolicum cholesterol oxidase in Escherichia coli by genetic modification Protein Expr Purif 12 347 352 10.1006/prep.1997.0855
23. AJ Smith CJW Brooks 1974 Application of cholesterol oxidase in the analysis of steroids J Chromatogr A 101 373 378 10.1016/S0021-9673(00)82854-X
24. A Vrielink LF Lloyd DM Blow 1991 Crystal structure of cholesterol oxidase from Brevibacterium sterolicum refined at 1.8 Å resolution J Mol Biol 219 533 554 10.1016/0022-2836(91)90192-9
25. MT Yazdi M Zahraei K Aghaepour N Kamranpour 2001 Purification and partial characterization of a cholesterol oxidase from Streptomyces fradiae Enzyme Microb Technol 28 410 414
26. C Yen-Rong H Hsin-Ho C Yi-Fang T Tsung-Yin L Wen-Hsiung 2006 Expression of a cholesterol oxidase gene from Arthrobacter simplex in Escherichia coli and Pichia pastoris Enzyme Microb Technol 39 854 860 10.1016/j.enzmictec.2006.01.018