Recombinant expression, purification, and characterization of ThmD, the oxidoreductase component of tetrahydrofuran monooxygenase

Archives of Biochemistry and Biophysics

Volumn 496, Issue 2, 2010, Pages 123-131

  Oppenheimer, Michelle ^a   Pierce, Brad S ^b   Crawford, Joshua A ^b   Ray, Keith ^a   Helm, Richard F ^a   Sobrado, Pablo ^a  

^a VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

^b UNIVERSITY OF TEXAS AT ARLINGTON   (United States)

Author keywords

Covalent flavin; Cytochrome c reductase; Flavin binding domain; Flavoenzyme; Iron sulfur center; Oxidoreductase; Tetrahydrofuran monooxygenase

Indexed keywords

2,6 DICHLOROPHENOLINDOPHENOL; CYTOCHROME C; FERRICYANIDE; FLAVINE ADENINE NUCLEOTIDE; MALTOSE BINDING PROTEIN; OXIDOREDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TETRAHYDROFURAN MONOOXYGENASE D; TETRAHYDROFURAN MONOOXYGENASE D FD; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; COVALENT BOND; ELECTRON SPIN RESONANCE; ENZYME ANALYSIS; ENZYME BINDING; ENZYME PURIFICATION; ENZYME STRUCTURE; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS;

BACTERIAL PROTEINS; ENZYME ACTIVATION; ENZYME STABILITY; ESCHERICHIA COLI; MIXED FUNCTION OXYGENASES; OXIDOREDUCTASES; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 77951023586     PISSN: 00039861     EISSN: 10960384     Source Type: Journal    
DOI: 10.1016/j.abb.2010.02.006     Document Type: Article

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