Expression and purification of the recombinant subunits of toluene/o-xylene monooxygenase and reconstitution of the active complex

European Journal of Biochemistry

Volumn 269, Issue 22, 2002, Pages 5689-5699

  Cafaro, Valeria ^a   Scognamiglio, Roberta ^a   Viggiani, Ambra ^a   Izzo, Viviana ^a   Passaro, Irene ^a   Notomista, Eugenio ^a   Dal Piaz, Fabrizio ^a   Amoresano, Angela ^a   Casbarra, Annarita ^a   Pucci, Piero ^a   Di Donato, Alberto ^a  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

Bioremediation; Electron transfer; Monooxygenase; Protein expression; Recombinant

Indexed keywords

4 METHYLCATECHOL; DNA; FLAVINE ADENINE NUCLEOTIDE; FLAVODOXIN; IRON; OXYGEN; OXYGENASE; PARA CRESOL; PROTEIN SUBUNIT; RECOMBINANT ENZYME; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE; SULFIDE; TOLUENE 2 XYLENE MONOOXYGENASE COMPLEX; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE;

ABSORPTION SPECTROSCOPY; AMINO ACID SEQUENCE; ARTICLE; DNA SEQUENCE; ELECTRON TRANSPORT; ENZYME ACTIVITY; ENZYME ANALYSIS; GENE SEQUENCE; GENETIC CODE; MOLECULAR BIOLOGY; MOLECULAR CLONING; NONHUMAN; OXIDATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN PURIFICATION; PSEUDOMONAS STUTZERI;

BIOCHEMISTRY; CHROMATOGRAPHY, GEL; DITHIONITE; ELECTRON TRANSPORT; ELECTRONS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENETIC VECTORS; IRON; MASS SPECTROMETRY; NAD; OXYGENASES; PLASMIDS; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SULFIDES;

PSEUDOMONAS; PSEUDOMONAS STUTZERI;

EID: 18744412095     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2002.03281.x     Document Type: Article

References (39)

1. Baggi, G., Barbieri, P., Galli, E. & Tollari, S. (1987) Isolation of a Pseudomonas stutzeri strain that degrades o-xylene. Appl. Environ. Microbiol. 53, 2129-2131.
2. Barbieri, P., Galassi, G. & Galli, E. (1989) Plasmid encoded mercury resistance in a Pseudomonas stutzeri strain that degrades o-xylene. FEMS Microbio. Ecol. 62, 375-384.
3. Bertoni, G., Bolognesi, F., Galli, E. & Barbieri, P. (1996) Cloning of the genes for and characterization of the early stages of toluene catabolism in Pseudomonas stutzeri OX1. Appl. Environ. Microbiol. 62, 3704-3711.
4. Bertoni, G., Martino, M., Galli, E. & Barbieri, P. (1998) Analysis of the gene cluster encoding toluene/o-xylene monooxygenase from Pseudomonas stutzeri OX1. Appl. Environ. Microbiol. 64, 3626-3632.
5. Chauhan, S., Barbieri, P. & Wood, T. (1998) Oxidation of trichloroethylene, 1,1-dichloroethylene, and chloroform by toluene/o-xylene monooxygenase from Pseudomonas stutzeri OX1. Appl. Environ. Microbiol. 64, 3023-3024.
6. Ryoo, D., Shim, H., Canada, K., Barbieri, P. & Wood, T.K. (2000) Aerobic degradation of tetrachloroethylene by toluene-o-xylene monooxygenase of Pseudomonas stutzeri OX1. Nature Biotechnol. 18, 775-778.
7. Johnson, G.R. & Olsen, R.H. (1995) Nucleotide sequence analysis of genes encoding a toluene/benzene-2-monooxugenase from Pseudomonas sp. strain JS150. Appl. Environ. Microbiol. 61, 3336-3346.
8. Olsen, R.H., Kukor, J.J. & Kaphammer, B. (1994) A novel toluene-3-monooxygenase pathway cloned from Pseudomonas pickettii PKO1. J. Bacterio. 176, 3748-3756.
9. Whited, G.M. & Gibson, D.T. (1991) Toluene-4-monooxygenase, a three component enzyma system that catalyzes the oxidation of toluene to p-cresol in Pseudomonas mendocina KR1. J. Bacteriolol. 173, 3010-3016.
10. Newman, L.M. & Wackett, L.P. (1995) Purification and characterization of Toluene 2-monooxygenase from Burkholderia cepacia G4. Biochemistry 34, 14066-14076.
11. Sullivan, J.P., Dickinson, D. & Chase, H.A. (1998) Methano-trophs, Methylosinus trichosporium OB3b, sMMO, and their application to bioremediation. Crit Rev. Microbiol. 24, 335-373.
12. Gallagher, S.C., Cammark, R. & Dalton, H. (1997) Alkene monooxygenase from Nocardia corallina B-276 is a member of the class of dinuclear iron proteins capable of stereospecific epoxygenation reactions. Eur. J. Biochem. 247, 635-641.
13. Scognamiglio, R., Notomista, E., Barbieri, P., Pucci, P., Dal Piaz, F., Tramontano, A. & Di Donato, A. (2001) Conformational analysis of putative regulatory subunit D of the toluene/o-xylene-monooxygenase complex from Pseudomonas stutzeri OX1. Protein Sci. 10, 482-490.
14. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edn. Cold Spring. Harbor Laboratory Press, Cold Spring Harbor, New York.
15. Sanger, F., Nicklen, S. & Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 76, 5653-5467.
16. Kunkel, T.A. (1987) Rapid and eUcient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82, 488-492.
17. Matsudaira, P. (1987) Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J. Biol. Chem. 262, 10035-10038.
18. Small, F.J. & Ensign, S.A. (1997) Alkene monooxygenase from Xanthobacter strain Py2. Purification and characterization of a four-component system central to the bacterial metabolism of aliphatic alkenes. J. Biol. Chem. 272, 24913-24920.
19. Fee, J.A., Findling, K.L., Yoshida, T., Hille, R., Tarr, G.E., Hearshen, D.O., Dunham, W.R., Day, E.P., Kent, T.A. & Munck, E. (1984) Purification and characterization of the Rieske iron-sulfur protein from Thermus thermaphilus. J. Biol. Chem. 259, 124-133.
20. Arenghi, F.L., Berlanda, D., Galli, E., Sello, G. & Barbieri, P. (2001) Organization and regulation of meta cleavage pathway gene for toluene and o-xylene derivative degradation in Pseudo-monas stutzeri OX1. Appl. Environ. Microbiol. 67, 3304-3308.
21. Batie, C.J., LaHaie, E. & Ballou, D.P. (1987) Purification and characterization of phthalate oxygenase and phthalate oxygenase reductase from Pseudomonas cepacia. J. Biol. Chem. 262, 1510-1518.
22. Rabinowitz, J.C. (1978) Analysis of acido-labile sulfide and sulphidryl groups. Methods Enzymol. 53, 275-277.
23. Brumby, P.E., Miller, R.W. & Massey, V. (1965) The content and possible catalytic significance of labile sulfide in some metallo-flavoproteins. J. Biol. Chem. 240, 2222-2228.
24. Laemmli, U. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
25. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248.
26. 15H resonances of the Rieske ferredoxin component of toluene 4-monooxygenase. Biochemistry 38, 727-739.
27. Shaw, J.P. & Harayama, S. (1992) Purification and characterisation of the NADH: Acceptor reductase component of xylene monooxygenase encoded by the TOL plasmid pWW0 of Pseudomonas putida mt-2. Eur. J. Biochem. 209, 51-61.
28. Colby, J. & Daton, H. (1978) Resolution of the methane mono-oxygenase of Methylococcus capsulatus (Bath) into three components. Biochem. J. 171, 461-468.
29. Yamaguchi, M. & Fujisawa, H. (1981) Reconstitution of iron-sulfur cluster of NADH-cytocrome c reductase, a component of benzoate 1,2-dioxygenase system from Pseudomonas arvilla C-1. J. Biol. Chem. 256, 6783-6787.
30. Pessione, E., Divari, S., Griva, E., Cavaletto, M., Rossi, G.L., Gilardi, G. & Giunta, C. (1999) Phenol hydroxylase from Acinetobacter radioresistens is a multicomponent enzyme. Eur. J. Biochem. 265, 549-555.
31. Colby, J. & Dalton, H. (1979) Characterization of the second prosthetic group of the flavoenzyme NADH-acceptor reductase (component C) of the methane mono-oxygenase from Methylococcus capsulatus Bath. Biochem. J. 177, 903-908.
32. Yamaguchi, M. & Fujisawa, H. (1978) Characterization of NADH-cytochrome c reductase, a component of benzoate 1,2-dioxygenase system from Pseudomonas arvilla C-1. J. Biol. Chem. 253, 8848-8853.
33. Pikus, J.D., Studts, J.M., Achim, C., Kauffmann, K.E., Munck, E., Steffan, R.J., McClay, K. & Fox, B.G. (1996) Recombinant toluene-4-monooxygenase: Catalytic and Mossbauer studies of the purified diiron and rieske components of a four-protein complex. Biochemistry 35, 9106-9119.
34. Rosenzweig, A.C., Frederick, C.A., Lippard, S.J. & Nordlund, P. (1993) Crystal structure of a bacterial non-heme iron hydroxylase that catalyses the biological oxidation of methane. Nature 366, 537-543.
35. Zhou, N.Y., Jenkins, A., Chan Kwo Chion, C.K. & Leak, D.J. (1998) The alkene monooxygenase from Xanthobacter Py2 is a binuclear non-haem iron protein closely related to toluene 4-monooxygenase. FEBS Lett. 430, 181-185.
36. Fox, B.G., Shanklin, J., Ai, J., Loehr, T. & Sanders-Loehr, J. (1994) Resonance Raman evidence for Fe-O-Fe center in Stearoyl-ACP desaturase. Primary sequence identity with other diiron-oxo proteins. Biochemistry 33, 12776-12786.
37. Fox, B.G., Liu, Y., Dege, J.E. & Lipscomb, J.D. (1991) Complex formation between the protein components of methane monooxygenase from Methylosinus trichosporium OB3b. Identification of sites of component interaction. J. Biol. Chem. 266, 540-550.
38. Fox, G.B., Froland, W.A., Dege, J.E. & Lipscomb, J.D. (1989) Methane monooxygenase from Methylosinus trichosporium OB3b. J. Biol. Chem. 264, 10023-10033.
39. Green, J. & Dalton, H. (1985) Protein B of soluble methane monooxygenase from Methylococcus capsulatus (Bath). A novel regulatory protein of enzyme activity. J. Biol. Chem. 260, 15795-15801.