Structural consequences of effector protein complex formation in a diiron hydroxylase

Proceedings of the National Academy of Sciences of the United States of America

Volumn 105, Issue 49, 2008, Pages 19194-19198

  Bailey, Lucas J ^a   McCoy, Jason G ^a   Phillips Jr , George N ^a   Fox, Brian G ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Crystal structure; Iron enzyme; Mechanism; Oxygenase

Indexed keywords

4 MONOOXYGENASE; CARBOXYLIC ACID; DIIRON HDYROXYLASE; OXYGENASE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CRYSTAL STRUCTURE; ENZYME STRUCTURE; PRIORITY JOURNAL; STOICHIOMETRY;

CATALYTIC DOMAIN; CRYSTALLOGRAPHY; IRON; METABOLISM; MIXED FUNCTION OXYGENASES; MOLECULAR WEIGHT; MULTIENZYME COMPLEXES; OXIDATION-REDUCTION; OXYGENASES; PROTEIN STRUCTURE, TERTIARY; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 58049215418     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0807948105     Document Type: Article

References (36)

1. Wallar BJ, Lipscomb JD (1996) Dioxygen activation by enzymes containing binuclear nonheme iron clusters. Chem Rev 96:2625-2658.
2. Leahy JG, Batchelor PJ, Morcomb SM (2003) Evolution of the soluble diiron monooxygenases. FEMS Microbiol Rev 27:449-479.
3. Mitchell KH, Studts JM, Fox BG (2002) Combined participation of hydroxylase active site residues and effector protein binding in a para to ortho modulation of toluene 4-monooxygenase regiospecificity. Biochemistry 41:3176-3188.
4. Paulsen KE, et al. (1994) Oxidation-reduction potentials of the methane monooxygenase hydroxylase component from methylosinus-trichosporium Ob3b. Biochemistry 33:713-722.
5. Lee SK, Nesheim JC, Lipscomb JD (1993) Transient intermediates of the methane monooxygenase catalytic cycle. J Biol Chem 268:21569-21577.
6. Froland WA, Andersson KK, Lee SK, Liu Y, Lipscomb JD (1992) Methane monooxygenase component-B and reductase alter the regioselectivity of the hydroxylase component-catalyzed reactions: A novel role for protein-protein interactions in an oxygenase mechanism. J Biol Chem 267:17588-17597.
7. Sazinsky MH, Lippard SJ (2005) Product bound structures of the soluble methane monooxygenase hydroxylase from Methylococcus capsulatus (Bath): Protein motion in the α-subunit. J Am Chem Soc 127:5814-5825.
8. McCormick MS, Sazinsky MH, Condon KL, Lippard SJ (2006) X-ray crystal structures of manganese(II)-reconstituted and native toluene/o-xylene monooxygenase hydroxylase reveal rotamer shifts in conserved residues and an enhanced view of the protein interior. J Am Chem Soc 128:15108-15110.
9. Sazinsky MH, Dunten PW, McCormick MS, DiDonato A, Lippard SJ (2006) X-ray structure of a hydroxylase-regulatory protein complex from a hydrocarbon-oxidizing multicomponent monooxygenase, Pseudomonas sp OX1 phenol hydroxylase. Biochemistry 45:15392-15404.
10. Merkx M, et al. (2001) Dioxygen activation and methane hydroxylation by soluble methane monooxygenase: A tale of two irons and three proteins. Angew Chem Int Ed 40:2782-2807.
11. Pikus JD, et al. (1996) Recombinant toluene 4-monooxygenase: Catalytic and Mössbauer studies of the purified diiron and Rieske components of a four-protein complex. Biochemistry 35:9106-9119.
12. Mitchell KH, Rogger CE, Gierhran T, Fox BG (2003) Insight into the mechanism of aromatic hydroxylation by toluene 4-monooxygenase through the use of specifically deuterated toluene and p-xylene. Proc Natl Acad Sci USA 100:3784-3789.
13. 2 activation. J Am Chem Soc 130:7098-7109.
14. Sazinsky MH, Merkx M, Cadieux E, Tang S, Lippard SJ (2004) Preparation and X-ray structures of metal-free, dicobalt and dimanganese forms of soluble methane monooxygenase hydroxylase from Methylococcus capsulatus (Bath). Biochemistry 43:16263-16276.
15. Lountos GT, Mitchell KH, Studts JM, Fox BG, Orville AM (2005) Crystal structures and functional studies of T4moD, the toluene 4-monooxygenase catalytic effector protein. Biochemistry 44:7131-7142.
16. Lloyd JS, Bhambra A, Murrell JC, Dalton H (1997) Inactivation of the regulatory protein B of soluble methane monooxygenase from Methylococcus capsulatus (Bath) by proteolysis can be overcome by a Gly to Gln modification. Eur J Biochem 248:72-79.
17. Chang SL, Wallar BJ, Lipscomb JD, Mayo KH (2001) Residues in Methylosinus trichosporium OB3b methane monooxygenase component B involved in molecular interactions with reduced- and oxidized-hydroxylase component: A role for the N terminus. Biochemistry 40:9539-9551.
18. Nordlund P, Eklund H (1993) Structure and function of the Escherichia coli ribonucleotide reductase protein R2. J Mol Biol 232:123-164.
19. 9 desaturase. Biochemistry 38:12197-12204.
20. Murray LJ, et al. (2007) Dioxygen activation at nonheme diiron centers: Oxidation of a proximal residue in the 1100W variant of toluene/o-xylene monooxygenase hydroxylase. Biochemistry 46:14795-14809.
21. Fox BG, Liu Y, Dege JE, Lipscomb JD (1991) Complex formation between the protein components of methane monooxygenase from Methylosinus-Trichosporium Ob3b - identification of sites of component interaction. J Biol Chem 266:540-550.
22. Blazyk JL, Gassner GT, Lippard SJ (2005) Intermolecular electron-transfer reactions in soluble methane monooxygenase: A role for hysteresis in protein function. J Am Chem Soc 127:17364-17376.
23. Andersson KK, Elgren TE, Que L, Lipscomb JD (1992) Accessibility to the active site of methane monooxygenase: The first demonstration of exogenous ligand binding to the diiron cluster. J Am Chem Soc 114:8711-8713.
24. Murray LJ, et al. (2007) Characterization of the arene-oxidizing intermediate in ToMOH as a diiron(III) species. J Am Chem Soc 129:14500-14510.
25. Murray LJ, Lippard SJ (2007) Substrate trafficking and dioxygen activation in bacterial multicomponent monooxygenases. Acc Chem Res 40:466-474.
26. Hemmi H, et al. (2001) Solution structure of the toluene 4-monooxygenase effector protein (T4moD). Biochemistry 40:3512-3524.
27. Lee SK, Lipscomb JD (1999) Oxygen activation catalyzed by methane monooxygenase hydroxylase component: Proton delivery during the O-O bond cleavage steps. Biochemistry 38:4423-4432.
28. Pikus JD, et al. (2000) Threonine 201 in the diiron enzyme toluene 4-monooxygenase is not required for catalysis. Biochemistry 39:791-799.
29. Rosenzweig AC, Nordlund P, Takahara PM, Frederick CA, Lippard SJ (1995) Geometry of the soluble methane monooxygenase catalytic diiron center in two oxidation states. Chem Biol 2:409-418.
30. Studts JM, et al. (2000) Optimized expression and purification of toluene 4-monooxygenase hydroxylase. Protein Expression Purif 20:58-65.
31. Otwinowski Z, Minor W (1997) The processing of X-ray difrraction data collected in oscillation mode. Methods Enzymol 276:307-326.
32. Vagin A, Teplyakov A (1997) MOLREP: An automated program for molecular replacement. J Appl Crystallogr 30:1022-1025.
33. Emsley P, Cowtan K (2004) Coot: Model-building tools for molecular graphics. Acta Crystallogr D 60:2126-2132.
34. Murshudov GN, Vagin AA, Dodson EJ (1997) Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr D 53:240-255.
35. Petrek M, et al. (2006) CAVER: A new tool to explore routes from protein clefts, pockets and cavities. BMC Bioinformatics 7:316.
36. DeLano WL (2002) The PyMOL Molecular Graphics System (DeLano Scientific, San Carlos, CA).