Towards the understanding of resistance mechanisms in clinically isolated trimethoprim-resistant, methicillin-resistant Staphylococcus aureus dihydrofolate reductase

Journal of Structural Biology

Volumn 170, Issue 1, 2010, Pages 93-97

  Frey, Kathleen M ^a   Lombardo, Michael N ^a   Wright, Dennis L ^a   Anderson, Amy C ^a  

^a UNIVERSITY OF CONNECTICUT   (United States)

Author keywords

Antifolates; Dihydrofolate reductase; Methicillin resistant Staphylococcus aureus; Trimethoprim

Indexed keywords

DIHYDROFOLATE REDUCTASE INHIBITOR; TRIMETHOPRIM;

ARTICLE; BACTERIAL STRAIN; BACTERIUM ISOLATION; CONTROLLED STUDY; CRYSTAL STRUCTURE; DRUG POTENCY; DRUG STRUCTURE; ENZYME INHIBITION; HYDROGEN BOND; HYDROPHOBICITY; IC 50; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; NONHUMAN; PRIORITY JOURNAL; STAPHYLOCOCCUS AUREUS; STRUCTURE ANALYSIS;

CLONING, MOLECULAR; CRYSTALLIZATION; FOLIC ACID ANTAGONISTS; METHICILLIN RESISTANCE; MODELS, MOLECULAR; MOLECULAR STRUCTURE; MUTATION, MISSENSE; STAPHYLOCOCCUS AUREUS; TETRAHYDROFOLATE DEHYDROGENASE; TRIMETHOPRIM; TRIMETHOPRIM RESISTANCE;

METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS;

EID: 77950520151     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2009.12.011     Document Type: Article

References (19)

1. Archer G.L., Coughter J.P., Johnston J.L. Plasmid-encoded trimethoprim resistance in staphylococci. Antimicrob. Agents Chemother. 1986, 29:733-740.
2. Beierlein J.M., Frey K.M., Bolstad D.B., Pelphrey P.M., Joska T.M., Smith A.E., Priestley N.D., Wright D.L., Anderson A.C. Synthetic and crystallographic studies of a new inhibitor series targeting Bacillus anthracis dihydrofolate reductase. J. Med. Chem. 2008, 51:7532-7540.
3. Bhat T.N., Cohen G.H. OMITMAP: an electron density map suitable for the examination of errors in a macromolecular model. J. Appl. Crystallogr. 1984, 17:244-248.
4. Bolstad D.B., Bolstad E.S., Frey K.M., Wright D.L., Anderson A.C. Structure-based approach to the development of potent and selective inhibitors of dihydrofolate reductase from Cryptosporidium. J. Med. Chem. 2008, 51:6839-6852.
5. Cheng Y., Prusoff W.H. Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction. Biochem. Pharmacol. 1973, 22:3099-3108.
6. Dale G.E., Then R.L., Stuber D. Characterization of the gene for chromosomal trimethoprim-sensitive dihydrofolate reductase of Staphylococcus aureus ATCC 25923. Antimicrob. Agents Chemother. 1993, 37:1400-1405.
7. Dale G.E., Broger C., Hartman P.G., Langen H., Page M.G., Then R.L., Stuber D. Characterization of the gene for the chromosomal dihydrofolate reductase (DHFR) of Staphylococcus epidermidis ATCC 14990: the origin of the trimethoprim-resistant S1 DHFR from Staphylococcus aureus?. J. Bacteriol. 1995, 177:2965-2970.
8. Dale G.E., Broger C., D'Arcy A., Hartman P.G., DeHoogt R., Jolidon S., Kompis I., Labhardt A.M., Langen H., Locher H., Page M.G., Stuber D., Then R.L., Wipf B., Oefner C. A single amino acid substitution in Staphylococcus aureus dihydrofolate reductase determines trimethoprim resistance. J. Mol. Biol. 1997, 266:23-30.
9. Drew R.H. Emerging options for treatment of invasive, multidrug-resistant Staphylococcus aureus infections. Pharmacotherapy 2007, 27:227-249.
10. Emsley P., Cowtan K. Coot: model-building tools for molecular graphics. Acta Crystallogr. D 2004, 60:2126-2132.
11. Frey K.M., Liu J., Lombardo M.N., Bolstad D.B., Wright D.L., Anderson A.C. Crystal structures of wild-type and mutant methicillin-resistant Staphylococcus aureus dihydrofolate reductase reveal an alternate conformation of NADPH that may be linked to trimethoprim resistance. J. Mol. Biol. 2009, 387:1298-1308.
12. Heaslet H., Harris M., Fahnoe K., Sarver R., Putz H., Chang J., Subramanyam C., Barreiro G., Miller J.R. Structural comparison of chromosomal and exogenous dihydrofolate reductase from Staphylococcus aureus in complex with the potent inhibitor trimethoprim. Proteins 2009, 76:706-717.
13. Kluytmans-Vandenbergh M.F., Kluytmans J.A. Community-acquired methicillin-resistant Staphylococcus aureus: current perspectives. Clin. Microbiol. Infect. 2006, 12:9-15.
14. Kollef M.H., Micek S.T. Methicillin-resistant Staphylococcus aureus: a new community-acquired pathogen?. Curr. Opin. Infect. Dis. 2006, 19:161-168.
15. Laskowski R.A., MacArthur M.W., Moss D.S., Thornton J.M. PROCHECK: a program to check the stereochemical quality of protein structures. J. Appl. Crystallogr. 1993, 26:283.
16. Liu J., Bolstad D.B., Smith A.E., Priestley N.D., Wright D.L., Anderson A.C. Structure-guided development of efficacious antifungal agents targeting Candida glabrata dihydrofolate reductase. Chem. Biol. 2008, 15:990-996.
17. Murshudov G.N., Vagin A.A., Dodson E.J. Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr. D 1997, 53:240-255.
18. Otwinowski Z., Minor W. Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol. 1997, 276:307-326.
19. Proctor R.A. Role of folate antagonists in the treatment of methicillin-resistant Staphylococcus aureus infection. Clin. Infect. Dis. 2008, 46:584-593.