The tension-transmitting 'clutch' in the mechanosensitive channel MscS

Nature Structural and Molecular Biology

Volumn 17, Issue 4, 2010, Pages 451-458

  Belyy, Vladislav ^a   Anishkin, Andriy ^a   Kamaraju, Kishore ^a   Liu, Naili ^a   Sukharev, Sergei ^a  

^a UNIVERSITY OF MARYLAND   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMMONIA; CYSTEINE; MEMBRANE PROTEIN; PROTEIN MSCS; SULFONIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; CHANNEL GATING; CRYSTAL STRUCTURE; ESCHERICHIA COLI; HYDROPHILICITY; LOSS OF FUNCTION MUTATION; MOLECULAR DYNAMICS; NONHUMAN; NUCLEOTIDE SEQUENCE; OSMOSIS; PHENOTYPE; PRIORITY JOURNAL; PROTEIN MODIFICATION; PROTEIN STABILITY; SIMULATION;

ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; ION CHANNELS; MODELS, MOLECULAR; MOLECULAR DYNAMICS SIMULATION; PROTEIN CONFORMATION;

ESCHERICHIA COLI;

EID: 77950519111     PISSN: 15459993     EISSN: 15459985     Source Type: Journal    
DOI: 10.1038/nsmb.1775     Document Type: Article

References (37)

1. Levina, N. et al. Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identifcation of genes required for MscS activity. EMBO J. 18, 1730-1737 (1999).
2. Sukharev, S. Purifcation of the small mechanosensitive channel of Escherichia coli (MscS): the subunit structure, conduction, and gating characteristics in liposomes. Biophys. J. 83, 290-298 (2002).
3. Okada, K., Moe, P.C. & Blount, P. Functional design of bacterial mechanosensitive channels. Comparisons and contrasts illuminated by random mutagenesis. J. Biol. Chem. 277, 27682-27688 (2002).
4. Pivetti, C.D. et al. Two families of mechanosensitive channel proteins. Microbiol. Mol. Biol. Rev. 67, 66-85 (2003).
5. Balleza, D. & Gomez-Lagunas, F. Conserved motifs in mechanosensitive channels MscL and MscS. Eur. Biophys. J. 38, 1013-1027 (2009).
6. Haswell, E.S. & Meyerowitz, E.M. MscS-like proteins control plastid size and shape in Arabidopsis thaliana. Curr. Biol. 16, 1-11 (2006).
7. Nakayama, Y., Fujiu, K., Sokabe, M. & Yoshimura, K. Molecular and electrophysio-logical characterization of a mechanosensitive channel expressed in the chloro-plasts of Chlamydomonas. Proc. Natl. Acad. Sci. USA 104, 5883-5888 (2007).
8. Rayavara, K. & Desai, S.A. Genetic disruption of a mechanosensitive ion channel in Plasmodium falciparum. Am. J. Trop. Med. Hyg. 79, 65-66 (2008).
9. Akitake, B., Anishkin, A. & Sukharev, S. The "dashpot" mechanism of stretch-dependent gating in MscS. J. Gen. Physiol. 125, 143-154 (2005).
10. Grajkowski, W., Kubalski, A. & Koprowski, P. Surface changes of the mechanosensitive channel MscS upon its activation, inactivation, and closing. Biophys. J. 88, 3050-3059 (2005).
11. Bass, R.B., Strop, P., Barclay, M. & Rees, D.C. Crystal structure of Escherichia coli MscS, a voltage-modulated and mechanosensitive channel. Science 298, 1582-1587 (2002).
12. Steinbacher, S., Bass, R., Strop, P. & Rees, D.C. Structures of the prokaryotic mechanosensitive channels MscL and MscS. in Mechanosensitive Ion Channels (ed. Hamill, P.O.) Ch. 1 (Academic Press, 2007).
13. Wang, W. et al. The structure of an open form of an E. coli mechanosensitive channel at 3.45 Å resolution. Science 321, 1179-1183 (2008).
14. Vasquez, V., Sotomayor, M., Cordero-Morales, J., Schulten, K. & Perozo, E. A structural mechanism for MscS gating in lipid bilayers. Science 321, 1210-1214 (2008).
15. Vasquez, V. et al. Three-dimensional architecture of membrane-embedded MscS in the closed conformation. J. Mol. Biol. 378, 55-70 (2008).
16. Akitake, B., Anishkin, A., Liu, N. & Sukharev, S. Straightening and sequential buckling of the pore-lining helices defne the gating cycle of MscS. Nat. Struct. Mol. Biol. 14, 1141-1149 (2007).
17. Anishkin, A. & Sukharev, S. Water dynamics and dewetting transitions in the small mechanosensitive channel MscS. Biophys. J. 86, 2883-2895 (2004).
18. Sotomayor, M. & Schulten, K. Molecular dynamics study of gating in the mechano-sensitive channel of small conductance MscS. Biophys. J. 87, 3050-3065 (2004).
19. Spronk, S.A., Elmore, D.E. & Dougherty, D.A. Voltage-dependent hydration and conduction properties of the hydrophobic pore of the mechanosensitive channel of small conductance. Biophys. J. 90, 3555-3569 (2006).
20. Nomura, T., Sokabe, M. & Yoshimura, K. Interaction between the cytoplasmic and transmembrane domains of the mechanosensitive channel MscS. Biophys. J. 94, 1638-1645 (2008).
21. Anishkin, A., Akitake, B. & Sukharev, S. Characterization of the resting MscS: modeling and analysis of the closed bacterial mechanosensitive channel of small conductance. Biophys. J. 94, 1252-1266 (2008).
22. Anishkin, A., Kamaraju, K. & Sukharev, S. Mechanosensitive channel MscS in the open state: modeling of the transition, explicit simulations, and experimental measurements of conductance. J. Gen. Physiol. 132, 67-83 (2008).
23. Eisenberg, D., Schwarz, E., Komaromy, M. & Wall, R. Analysis of membrane and surface protein sequences with the hydrophobic moment plot. J. Mol. Biol. 179, 125-142 (1984)
24. Kullback, S. Information Theory and Statistics (Dover Publications, Inc., Mineola, New York, USA, 1997).
25. Beckstein, O. & Sansom, M.S. The infuence of geometry, surface character, and fexibility on the permeation of ions and water through biological pores. Phys. Biol. 1, 42-52 (2004).
26. Booth, I.R. et al. Structure-function relations of MscS. in Mechanosensitive Ion Channels (ed. Hamill, P.O.) Ch. 10 (Academic Press, 2007).
27. Sukharev, S., Akitake, B. & Anishkin, A. The bacterial mechanosensitive channel MscS: emerging principles of gating and modulation. in Mechanosensitive Ion Channels (ed. Hamill, P.O.) Ch. 9 (Academic Press, 2007).
28. Nomura, T., Sokabe, M. & Yoshimura, K. Lipid-protein interaction of the MscS mechanosensitive channel examined by scanning mutagenesis. Biophys. J. 91, 2874-2881 (2006).
29. White, S.H. & Wimley, W.C. Membrane protein folding and stability: physical principles. Annu. Rev. Biophys. Biomol. Struct. 28, 319-365 (1999).
30. Zhou, X. et al. Yeast screens show aromatic residues at the end of the sixth helix anchor transient receptor potential channel gate. Proc. Natl. Acad. Sci. USA 104, 15555-15559 (2007).
31. Su, Z. et al. Yeast gain-of-function mutations reveal structure-function relationships conserved among different subfamilies of transient receptor potential channels. Proc. Natl. Acad. Sci. USA 104, 19607-19612 (2007).
32. Chiang, C.S., Anishkin, A. & Sukharev, S. Gating of the large mechanosensitive channel in situ: estimation of the spatial scale of the transition from channel population responses. Biophys. J. 86, 2846-2861 (2004).
33. Phillips, J.C. et al. Scalable molecular dynamics with NAMD. J. Comput. Chem. 26, 1781-1802 (2005).
34. MacKerell, A.D. et al. All-atom empirical potential for molecular modeling and dynamics studies of proteins. J. Phys. Chem. B 102, 3586-3616 (1998).
35. Darden, T., York, D. & Pedersen, L. Particle mesh Ewald-an n·log(n) method for Ewald sums in large systems. J. Chem. Phys. 98, 10089-10092 (1993).
36. Humphrey, W., Dalke, A. & Schulten, K. VMD: visual molecular dynamics. J. Mol. Graph. 14, 33-38 (1996).
37. Jorgensen, W.L., Chandrasekhar, J. & Madura, J.D. Comparison of simple potential functions for simulating liquid water. J. Chem. Phys. 79, 926 (1983).